선택한 단어 수는 입니다.
최소 단어 이상 선택하여야 합니다.
최소 단어 이상 선택하여야 합니다.
선택한 단어 수는 30입니다.
최대 10 단어까지만 선택 가능합니다.
최대 10 단어까지만 선택 가능합니다.
제안 방법
- 1A). The anti-inflammatory effects of the FAJE were identified by measuring the changes in the NO generation rates. When inflammatory reactions were induced to RAW264.
- In the experiment, the normal group in which osteoarthritis was not induced. The osteoarthritis-induced group, and the groups in which osteoarthritis was induced and 100, 200, or 400 mg/kg of FAJE was administered were organized with six rats per group. FAJE was orally administered at 10 mL/kg for four weeks using sondes.
- The collected knee joints were washed with a physiological saline solution and pulverized into small pieces; RIPA buffer was added to the small pieces of the knee joints to extract proteins. The proteins were subjected to centrifugation for 15 minutes at 4℃ and 15,000 rpm and the optical density of the supernatant was measured using ELISA kit (Mybiosource, San Diego, CA, USA) to measure the production rates of MMP-2 and MMP-9.
- Two or three rats were put into each of polycarbonate breeding cages and fed freely with sterilized feed and drinking water; the rats were used in the experiment after undergoing an acclimatization process for one week in an environment with light-dark cycles of 12 h (07:00-19:00), illumination intensity of 200-300 lux, temperature of 22±2℃, and humidity of 50±5%.
대상 데이터
- A total of 30 male SD rats aged 8 weeks weighing 250-300 g bought from Daehan Bio Link Co., Ltd. (Eumseong, Korea) were used as experimental animals. All animal experiments were conducted using protocols approved by EBO Animal Research Ethics Committee (EBOA201603).
- RAW264.7 cells bought from ATCC (American Type Culture Collection, Manassas, VA, USA) were used. A culture medium was prepared by adding 100 U/mL penicillin and 100 μg/mL streptomycin to DMEM (Gibco, Grand Island, NY, USA) containing 10% FBS (Gibco) and used during culture in a 5% CO2 incubator at 37℃.
- The Achyranthes japonica (Miq.) Nakai’s roots used in the present experiment were bought around May 2014 in Andong, Gyeongbuk, and washed with water before use.
데이터처리
- All data are presented as means and deviations and the statistical analyses were conducted using the SPSS program (IBM Corporation, Armonk, NY, USA). One-way analyses of variation (ANOVA) were conducted.
- In cases where significance was observed in ANOVA, Dunnett’s t-tests were conducted to identify experimental groups with significant differences from the control group; Duncan’s multiple range tests were conducted to see whether there were differences between the experimental groups (significance level; both sides 5%).
- Values with different letters are significantly different at p<0.05 by Duncan’s multiple range test.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.