[논문]Morphological, molecular, and chromosomal identification of dwarf haploid parthenosporophytes of Tauya basicrassa (Phaeophyceae, Laminariales) from the Sea of Okhotsk

Klochkova, Tatyana A.; Klochkova, Nina G.; Yotsukura, Norishige; Kim, Gwang Hoon

doi:10.4490/algae.2017.32.1.31

Morphological, molecular, and chromosomal identification of dwarf haploid parthenosporophytes of Tauya basicrassa (Phaeophyceae, Laminariales) from the Sea of Okhotsk 원문보기

Algae, v.32 no.1, 2017년, pp.15 - 28

Klochkova, Tatyana A. (Department of Biology, Kongju National University) , Klochkova, Nina G. (Kamchatka State Technical University) , Yotsukura, Norishige (Field Science Center for Northern Biosphere, Hokkaido University) , Kim, Gwang Hoon (Department of Biology, Kongju National University)

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Morphological, molecular and chromosomal studies were carried out on Tauya basicrassa, an endemic kelp species distributed on the northern continental coast of the Sea of Okhotsk in Russia. The sporophytes of T. basicrassa grow up to 3-6 m long, 1.8-2.2 m wide, and 6.5-7 kg wet weight. The thallus has a blade with very thick narrow basal portion and thinner and much broader upper portion, which usually splits into 3 bullated lobes. A dwarf laminariacean alga, which did not show any morphological similarity to the other species of the order Laminariales, was found from the same locality. The blade of this alga is thin and soft, reached 26-34 cm long and 6-6.5 cm wide and had 4 longitudinal rows of bullations that covered the entire blade. Molecular analysis showed that the dwarf alga has 100% sequence identity in plastid-encoded RuBisCo spacer, mitochondrial cytochrome c oxidase subunit 1 and nuclear-encoded rDNA genes with normal sporophytes of T. basicrassa, indicating that they are different life forms of the same species. Fluorescent DAPI staining showed that the nucleus in the normal sporophyte was 50-65% larger than those of the dwarf ones. Chromosome count using acetocarmine staining showed n = ca. 20 for the normal sporophytes of T. basicrassa and n = ca. 10 for the dwarf one. These results suggest that the dwarf thallus is a haploid parthenosporophyte of T. basicrassa, which developed in nature. This is the first evidence of parthenosporophytes of the laminariacean algae occurring naturally in the field.

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Numerous glandular cells can be seen (dashed arrow). (H) Cross-section of mucilage duct. Scale bars represent: A & E, 10 cm; B-D, 5 cm; F, 500 µm; G, 200 µm; H, 100 µm.

제안 방법

Because the chromosomes spread out in three dimensions, the numbers were counted by tracing and marking individual chromosomes through stacks of consecutively taken images (Mann and Poulĺčková 2010). First, the labeling was accomplished for all nuclei in both types of plants and then counting was performed.
Unexpectedly, they turn out to be genetically identical, indicating that they are different life forms of the same species. Ploidy levels of both morphotypes were analyzed using fluorescent DAPI staining and chromosome counting.

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2013). Collection sites inside Taui Bay included Nedorazumeniya Island (type locality) and Svetlaya bay (Fig. 1). We also re-investigated type specimen (Fig.

성능/효과

(A) Mature plant freshly extracted from the sea. (B) Stipe is short and flattened in the upper portion, and haptera are rigid and branched as seen on the cross-section. (C & D) Juvenile plants at different state of maturity, showing two (C) and four (D) rows of developing bullations.
Fluorescent DAPI staining showed that the volume of nuclei in the normal sporophytes of T. basicrassa was by 50-65% larger than in the parthenosporophytes (Fig. 8A & B).
Krupnova). It should be noted that the type specimen is in a fragile condition, because unlike other kelp specimens, large thin blades of T. basicrassa are very fragile when dried and they crack and crumble easily. We also studied another specimen of T.

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