Herbicidin A is a potent herbicide against dicotyledonous plants as well as an antibiotic against phytopathogens. In this study, fermentation parameters for herbicidin A production in submerged culture of Streptomyces scopuliridis M40 were investigated. The herbicidin A concentration varied with the...
Herbicidin A is a potent herbicide against dicotyledonous plants as well as an antibiotic against phytopathogens. In this study, fermentation parameters for herbicidin A production in submerged culture of Streptomyces scopuliridis M40 were investigated. The herbicidin A concentration varied with the C/N ratio. High C/N ratios (>4) resulted in a herbicidin A production of more than 900 mg/l, whereas maximally 600 mg/l was obtained at ratios between 1 and 3.5. In 5-L batch fermentation, there was a positive correlation between the oxygen uptake rate (OUR) and herbicidin A production. Once the OUR increased, the substrate consumption rate increased, leading to an increase in volumetric productivity. Mechanical shear force affected the hyphal morphology and OUR. When the medium value of hyphal size ranged from 150 to $180{\mu}m$, high volumetric production of herbicidin A was obtained with OUR values >137mg $O_2/l{\cdot}h$. The highest herbicidin A concentration of 956.6 mg/l was obtained at 500 rpm, and coincided with the highest relative abundance of hyphae of $100-200{\mu}m$ length and the highest OUR during cultivation. Based on a constant impeller tip speed, which affects hyphal morphology, herbicidin A production was successfully scaled up from a 5-L jar to a 500-L pilot vessel.
Herbicidin A is a potent herbicide against dicotyledonous plants as well as an antibiotic against phytopathogens. In this study, fermentation parameters for herbicidin A production in submerged culture of Streptomyces scopuliridis M40 were investigated. The herbicidin A concentration varied with the C/N ratio. High C/N ratios (>4) resulted in a herbicidin A production of more than 900 mg/l, whereas maximally 600 mg/l was obtained at ratios between 1 and 3.5. In 5-L batch fermentation, there was a positive correlation between the oxygen uptake rate (OUR) and herbicidin A production. Once the OUR increased, the substrate consumption rate increased, leading to an increase in volumetric productivity. Mechanical shear force affected the hyphal morphology and OUR. When the medium value of hyphal size ranged from 150 to $180{\mu}m$, high volumetric production of herbicidin A was obtained with OUR values >137mg $O_2/l{\cdot}h$. The highest herbicidin A concentration of 956.6 mg/l was obtained at 500 rpm, and coincided with the highest relative abundance of hyphae of $100-200{\mu}m$ length and the highest OUR during cultivation. Based on a constant impeller tip speed, which affects hyphal morphology, herbicidin A production was successfully scaled up from a 5-L jar to a 500-L pilot vessel.
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제안 방법
The flow rate and detection wavelength were 1 ml/min and 254 nm, respectively. A calibration curve was constructed by plotting the peak area of five different standards of herbicidin A (i.e., 50, 100, 200, 400, and 800 mg/l) against the corresponding concentrations of standard solutions, showing the best linearity (R2 = 0.999). With the calibration curve, the peak area of samples was converted into herbicidin A concentration.
The optimum C/N ratio of the fermentation medium was investigated in flasks, and fermentation parameters, including dissolved oxygen uptake rate and hyphal size distribution, were investigated under various agitation rates in 5-L jar fermenters. After gaining a full understanding of the characteristics of S. scopuliridis M40 with regard to herbicidin A production, the production was scaled up from a 5-L jar to a 500-L pilot vessel.
대상 데이터
S. scopuliridis KR-001 [15], which was isolated from woodlands in Daejeon, Republic of Korea, was used in this study. For strain improvement, UV mutagenesis was performed according to the protocol of Miller [24] with slight modification.
The production was scaled up from a 5-L jar to a 500-L pilot vessel based on a constant impeller tip speed, which had an effect on oxygen supply and hyphal length of S. scopuliridis M40. An agitation rate of 500 rpm in a 5-L jar corresponded to 150 rpm in a 50-L pilot and 90 rpm in a 500-L pilot, respectively (Table 3).
데이터처리
Analysis of variance (ANOVA) tests were used to determine the significant differences between treatments at p < 0.05 using Tukey’s HSD test.
성능/효과
Considering that the highest production of herbicidin A was obtained at the highest relative abundance of 100–200 µm on the basis of morphology, the agitation rate of 500 rpm was best for herbicidin A production.
The mutant strain showing the highest productivity was selected and named “M40.” The herbicidin A concentration produced by S. scopuliridis M40 was 308.7 ± 23.3 mg/l, which was 3 times higher than that produced by S. scopuliridis KR-001 (103.9 ± 9.5 mg/l).
참고문헌 (35)
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