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저수온 스트레스시 자바리, Epinephelus bruneus에서의 vitamin E의 생리적 효과
Physiological effects of dietary vitamin E on kelp grouper, Epinephelus bruneus under low water temperature stress 원문보기

水産海洋敎育硏究 = Journal of fisheries and marine sciences education, v.29 no.3 = no.87, 2017년, pp.636 - 645  

임상구 (국립수산과학원) ,  길현우 (한국해양대학교) ,  이태호 (한국해양대학교) ,  박인석 (한국해양대학교)

Abstract AI-Helper 아이콘AI-Helper

The physiological response of the longtooth grouper, Epinephelus bruneus to low water temperature (LWT) stress while on three different concentrations of dietary vitamin E (1 mg/diet g, 5 mg/diet g, and 10 mg /diet g) were investigated. Plasma cortisol, glucose, and aspartate aminotransferase (AST) ...

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제안 방법

  • The low water temperature stress test commenced after a consistent diet for two weeks. Each sample was randomly selected and experimented in a rectangular glass tank (Dimensions W109 x L69 x H47 cm) followed by the admission of 50 longtooth groupers. Water temperature in tanks were decreased at 1 °C every hour in order to allow for acclimatization and to avoid possible sudden death from shock.
  • In order to determine the physiological responses of stressed fish with added vitamin E in a dietary supplement, levels of glucose, cortisol, alanine aminotransferase (ALT), and aspartate aminotransferase (AST) in their blood plasma were analyzed. Blood glucose among stressed fish is easy to measure and a good indicator of the second stress response (Wednmeyer et al.

대상 데이터

  • The experiments were performed in triplicate. Data were analyzed by ANOVA with the SPSS statistical package (SPSS 9.

데이터처리

  • The experiments were performed in triplicate. Data were analyzed by ANOVA with the SPSS statistical package (SPSS 9.0, SPSS Inc., USA). Means were performed by using Duncan’s multiple range test and significant difference were found using P<0.
  • Means were performed by using Duncan’s multiple range test and significant difference were found using P<0.05 (Duncan, 1955).

이론/모형

  • The glucose, ALT, and AST concentrations were analyzed using blood automatic analysis (Boehringer Mannhein Reflotron, Germanry). The cortisol concentration was measured using the method of rabbit anti-Cortisol-3-CMO-BSA antibody (Cosmo-Bio Co. Ltd., Tokyo, NH, USA) and radiation mark 1,2,5,6-H3 - cortisol (Amersham Life Science, England).
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참고문헌 (27)

  1. Barton, Bruce A. & Iwama, George K.(1991). Physiological changes in fish from stress in aquaculture with emphasis on the response and effects of corticosteroids. Annual Review of Fish Diseases, 1, 3-26. 

  2. Blaszer, Vicki S.(1992). Nutrition and disease resistance in fish. Annual Review of Fish Diseases, 1, 309-323. 

  3. Das, Pratap Chandra.Ayyappan, S..Jena, J.K. & Das, Basanta Kumar(2004). Nitrite toxicity in Cirrhinus mrigala (Ham): acute toxicity and sub-lethal effect in selected haematological parameters. Aquaculture, 235, 633-644. 

  4. Duncan, David B.(1955). Multiple-range and multiple F tests. Biometrics, 1, 1-42. 

  5. Gamperl, A.K..Wilkinson, M. & Boutilier, R.G.(1994). ${\beta}$ -Adrenoreceptors in the trout (Oncorhynchus mykiss) heart: characterization, quantification & effects of repeated catecholamine exposure. General and Comparative Endocrinology, 95, 259-272. 

  6. Halver, John E.(2002). The vitamins. In: Halver John E., Hardy Ronald W. (eds), Fish Nutrition. Academic Press, San Diego, CA, pp. 61-141. 

  7. Hamre, K..Berge, R.K. & Lie, O.(1998). Turnover of ${\alpha}$ -, r-, and ${\delta}$ -tocopherol and distribution in subcellular and lipoprotein fractions indicate presence of a hepatic tocoperol binding protein in Atlanctic salmon (Salmo salar L.). Fish Physiology and Biochemistry, 18, 71-83. 

  8. Hur, Jun Wook & Habibi, Hamid R.(2007). Physiological response and hematological characteristics of goldfish (Carassius auratus) to water temperature shock. Korean Journal of Ichthyology, 19, 93-100. 

  9. Lall, Santosh P. & Olivier, George(1993). Role of micronutrients in immune response and disease resistance in fish. In: Kaushik Sadasivam, Luquet P (eds) Fish Nutrition in Practice. Institut national de la recherche agronomique, Paris, pp. 101-118. 

  10. Mazeaud, Madeleine M..Mazeaud, Frederic & Donaldson, Edward M.(1977). Primary and secondary effects of stress in fish: Some new data with a general review. Transaction of the American Fisheries Society, 106, 201-212. 

  11. Montero, Daniel.Tort, Lluis.Izquierdo, Marisol S..Robaina, L. & Vergara, J. M.(1998). Depletion of serum alternative complement pathway activity in gilthead seabream caused by ${\alpha}$ -tocopherol and n - 3H UFA dietary deficiencies. Fish Physiology and Biochemistry, 18, 399-407. 

  12. Mudron, P..Kovac, G..Bartko, P..Choma, J. & Zezula, I.(1996). Effect of vitamin E on the cortisol and lactate levels and the acid base equilibrium of calves subjected to transport stress. Veterinary Medicine, 41, 71-76. 

  13. Murai, Takeshi & Andrews, James W.(1974). Interactions of dietary ${\alpha}$ -tocopherol, oxidized menhadeniol and ethoxyquin on channel catfish (Ictalurus punctatus). Journal of Nutrition, 104, 1416-1431. 

  14. National Research Council (NRC).(1993). Nutrient Requirement of Fish. National Academic Press, Washington, DC. 

  15. Ortuno, J..Esteban, Angeles & Meseguer, Jose(2000). High dietary intake of ${\alpha}$ -tocopherol acetate enhances the non-specific immune response of gilthead seabream (Sparus aurata L.). Fish and Shellfish Immunology, 10, 293-307. 

  16. Park, Min Ouk & Park, In-Seok(2009). Long-term effects of passive integrated transponder (PIT) tagging on the kelp grouper, Epinephelus bruneus. Journal of Fish Biology, 74, 285-288. 

  17. Park, Min Ouk.Hur, Woo June.Im, Soo-Yeon.Seol, Dong-Won.Lee, Jin Hwan & Park, In-Seok(2008). Anesthetic efficacy and physiological responses to clove oil-anesthetized kelp grouper, Epinephelus bruneus. Aquaculture Research, 39, 877-884. 

  18. Pickering, A.D. & Pottinger, Tom G.(1989). Stress responses and diseases resistance in salmonid fish: effects of chronic elevation of plasma cortisol. Fish Physiology and Biochemistry, 7, 253-258. 

  19. Pickering, A.D. & Stewart, A.(1984). Acclimation of the interregnal tissue of the brown trout, Salmo trutta L., to chronic crowding stress. Journal of Fish Biology 24, 731-740. 

  20. Thompson, I..White, Andy.Fletcher, T.C..Houlihan, Dominic F. & Secombes, C.J.(1993). The effect of stress on the immune response of Atlantic salmon (Salmo salar) fed diets containing different amounts of vitamin C. Aquaculture, 114, 1-18. 

  21. Tort, Lluis.Gomez, Eladi.Montero, Daniel & Sunyer, J.Oriol(1996). Serum haemolytic and agglutinating activity as indicators of fish immunocompetence: their suitability in stress and dietary studies. Aquaculture International, 4, 31-41. 

  22. Wantanabe, Takeshi.Takeuchi, Toshio.Matsui, Masami.Ogino, Chinkichi & Kawabata, Toshiharu(1977). Effect of ${\alpha}$ -tocopherol deficiency on carp: VII. The relationship between dietary levels of linoleate and ${\alpha}$ -tocopherol requirement. Bulletin of the Japanese Society for the Science of. Fish, 43, 935-946. 

  23. Wastson, Ronald R. & Petro, Thomas M.(1982). Cellular immune responses, corticosteroid levels, and resistance to Listeria monocytogenes and murine leukemia in mice fed a high vitamin E diet. Annals of the New York Academy of Science, 205-208. 

  24. Wedemeyer, G. A..Barton, Bruce A. & McLeay, D.J.(1990). Stress and acclimation. In: Schreck Carl B., Moyle Peter B. (eds) Methods for Fish Biology, American Fish. Society, Bechesda, Maryland, pp. 451-489. 

  25. Wendelaar Bonga, Sjoerd E.(1997). The stress response in fish. Physiological Reviews, 77, 591-625. 

  26. Wilson, Robert P..Bowser, Paul R. & Poe, William E.(1984). Dietary vitamin E requirement of fingerling channel catfish. Journal of Nutrition, 114, 2053-2058. 

  27. Yeo, In-Kyu. & Yang, Jpung-Hwan(2004). Physiological studies on acute water-temperature stress of olive flounder (Paralichthys olivacius). Korean Journal of Ichthyology, 16, 19-26. 

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