Ptaquiloside는 고사리에 들어 있는 발암성 물질이다. 본 연구는 다양한 조리법에 따라 고사리에 함유된 ptaquiloside을 측정해 보고자 하였다. 조리법은 가열, 물에 침지, 물교환 횟수 등이었다. 알칼리 가수분해와 용매분획은 ptaquiloside를 화학적으로 안정한 물질인 pterosin B로 전환시켜 주었다. Pterosin B 함량은 이동상용매로 3 mM ammonium과 메탄올 용매를 사용하여 UPLC-MS/MS로 분석하였다. 조리과정이 없는 생고사리의
The ptaquiloside is a carcinogenic compound from bracken fern (Pteridium aquilinum L). This study was to evaluate the content of ptaquiloside in bracken fern by various processing methods. The processing methods were heated and immersed time, water exchange number, and so on. Akali hydrolysis and solvent fractionation of ptaquiloside in bracken fern leads to the non-toxic and chemically stable pterosin B. The contents of pterosin B was analyzed by UPLC-MS/MS on mobile phase 3 mM ammonium acetate and methanol. The contents of total pterosin B in non-processing bracken fern in water extraction was 81.0 mg/kg and toxic ptaquiloside of them was 46.4 mg/kg. The heating time of 5 minutes removed 60% about the contents of pterosin B in the bracken fern, and two-thirds of them were already non-toxic pterosin B, namely were not transfered from ptaquiloside. Additional immersed time (12h), the pterosin B in bracken fern was 10 mg/kg, it was removed 87.6% and once every hour, water exchange times were removed 99.5% in comparison with them of untreated bracken fern and two-thirds of them were non-toxic pterosin B. To remove of ptaquiloside in bracken fern, heat, immersion, and water exchange times shall be carried out simultaneously.
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