한국 퉁가리속 고유종 동방자가사리 Liobagrus hyeongsanensis의 핵형 분석 Karyotype Analysis of an Endemic Korean Torrent Catfish Liobagrus hyeongsanensis(Siluriformes: Amblycipitidae)원문보기
최근 신종으로 발표된 동방자가사리 Liobagrus hyeongsanensis의 핵형 분석을 위해 경상북도 경주시 양북면 일대에서 암컷 10마리와 수컷 5마리를 채집하였다. 동방자가사리의 염색체 수는 2n=42이었으며, 핵형은 30개의 중부염색체와 12개의 차중부염색체로 구성되어 있었다. FN 값은 84였으며, 암수 간 성적이형과 배수체는 관찰되지 않았다. 동방자가사리는 퉁가리속의 자가사리, 섬진자가사리와 염색체 수는 같았으나 핵형에서 차이를 보였다. 이러한 핵형의 차이는 지리적 격리로 인한 로버트슨 전좌(Robertsonian rearrangement)와 관련 있는 것으로 생각된다.
최근 신종으로 발표된 동방자가사리 Liobagrus hyeongsanensis의 핵형 분석을 위해 경상북도 경주시 양북면 일대에서 암컷 10마리와 수컷 5마리를 채집하였다. 동방자가사리의 염색체 수는 2n=42이었으며, 핵형은 30개의 중부염색체와 12개의 차중부염색체로 구성되어 있었다. FN 값은 84였으며, 암수 간 성적이형과 배수체는 관찰되지 않았다. 동방자가사리는 퉁가리속의 자가사리, 섬진자가사리와 염색체 수는 같았으나 핵형에서 차이를 보였다. 이러한 핵형의 차이는 지리적 격리로 인한 로버트슨 전좌(Robertsonian rearrangement)와 관련 있는 것으로 생각된다.
For the karyotype analysis of Liobagrus hyeongsanensis, an endemic Korean torrent catfish, ten females and five males were used from Yangbuk-myeon, Gyeongju-si, Gyeongsangbukdo, Korea. The diploid number of chromosomes was 42, and its karyotype consisted of 30 metacentrics and 12 submetacentrics; 84...
For the karyotype analysis of Liobagrus hyeongsanensis, an endemic Korean torrent catfish, ten females and five males were used from Yangbuk-myeon, Gyeongju-si, Gyeongsangbukdo, Korea. The diploid number of chromosomes was 42, and its karyotype consisted of 30 metacentrics and 12 submetacentrics; 84 FN (fundamental number), with having no polyploidy and sexual dimorphism. The chromosome number was the same as other closely-related species, L. mediadiposalis and L. somjinensis, but their karyotypes showed a clear difference by species. Such result may be related to chromosomal rearrangements by Robertsonian rearrangement with geographical isolation.
For the karyotype analysis of Liobagrus hyeongsanensis, an endemic Korean torrent catfish, ten females and five males were used from Yangbuk-myeon, Gyeongju-si, Gyeongsangbukdo, Korea. The diploid number of chromosomes was 42, and its karyotype consisted of 30 metacentrics and 12 submetacentrics; 84 FN (fundamental number), with having no polyploidy and sexual dimorphism. The chromosome number was the same as other closely-related species, L. mediadiposalis and L. somjinensis, but their karyotypes showed a clear difference by species. Such result may be related to chromosomal rearrangements by Robertsonian rearrangement with geographical isolation.
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대상 데이터
15 specimens(10 females, 5 males) of Liobagrus hyeongsanensis were collected in Daejongcheon of Yangbukmyeon, Gyeongju-si, Gyeongsangbuk-do, Republic of Korea (35°49ʹ58ʺ N, 129°24ʹ41ʺ E), where the holotype specimen is distributed, from May to June 2016, ranging 51 to 90 mm in standard length.
15 specimens(10 females, 5 males) of Liobagrus hyeongsanensis were collected in Daejongcheon of Yangbukmyeon, Gyeongju-si, Gyeongsangbuk-do, Republic of Korea (35°49ʹ58ʺ N, 129°24ʹ41ʺ E), where the holotype specimen is distributed, from May to June 2016, ranging 51 to 90 mm in standard length. The specimens are kept alive and transported to the laboratory at Department of Biological Sciences, Chonbuk National University. For chromosomal and karyotype analysis, 1% concanavalin A solution was injected into the abdominal cavity at a dose of 0.
이론/모형
The minced cells were gathered by centrifugation at 1,100 rpm, for 10 mins and fixed in a fresh chilled Carnoy’s solution (Methanol : Acetic acid in 3 : 1 ratio). The cells obtained from the above procedure were settled on the slide according to the flame drying method (Ojima et al., 1972). The slides were stained with 4% Giemsa solution for 8 minutes, and washed by distilled water, and dried at room temperature.
후속연구
1, may have sex chromosomes due to an unmatched pair in its males. Therefore, this study will be focused on the chromosome number and karyotype of L. hyeongsanensis as part of acquisition of basic data for phylogenetic and evolutionary information of the genus Korean Liobagrus.
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