[논문]양어 사료첨가제로서의 유산균 발효 개똥쑥의 항산화 및 항균활성

이아란; 우개민; 강수경; 한성구; 이봉주; 김수기

doi:10.5352/jls.2017.27.6.652

양어 사료첨가제로서의 유산균 발효 개똥쑥의 항산화 및 항균활성
Antioxidant and Antibacterial Activities of Lactobacillus-fermented Artemisia annua L. as a Potential Fish Feed Additive 원문보기

생명과학회지 = Journal of life science, v.27 no.6 = no.206, 2017년, pp.652 - 660

이아란 (건국대학교 동물자원과학과) , 우개민 (건국대학교 동물자원과학과) , 강수경 (건국대학교 축산식품공학과) , 한성구 (건국대학교 축산식품공학과) , 이봉주 (국립수산과학원 사료연구센터) , 김수기 (건국대학교 동물자원과학과)

초록
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최근 양어에서는 전염병, 환경오염, 어분 가격 상승 등으로 어려움을 가지고 있다. 본 연구는 쑥에서 분리한 L. plantarum SK3494를 이용하여 전통 약초인 개똥쑥을 발효시켜 in vitro 생리활성을 측정하고 어류용 사료첨가제로서의 가능성을 연구하였다. 개똥쑥의 발효시 유산균수는 9.38 log10 CFU/ml이며, pH는 4.1로 나타났다. 발효개똥쑥의 항산화활성은 DPPH 측정과 MAC-T 세포 내 과산화물 감소 실험에서 뛰어난 효과를 보였다. 발효개똥쑥은 어류 병원균인Photobacterium damselae subsp. damselae와 Vibrio ichthyoenteri에 대하여 강한 항균활성을 나타내었다. 따라서 유산균을 이용한 개똥쑥 발효물은 양식용 사료첨가제로써 가능성을 가지고 있는 것으로 판단되었다.

Abstract ▼ AI-Helper

Fermented medical herbs using Lactobacilli have attracted significant attention due to their enhanced biological activities. A traditional medicinal plant, Artemisia annua L., was fermented using a probiotic strain, L. plantarum SK3494. The strain was isolated from Artemisia princeps var. orientalis and molecularly identified through sequence similarities and phylogenetic tree analysis. The antioxidant activity of L. plantarum-fermented A. annua L. (LFA) was determined using the DPPH free radical scavenging assay. Cellular antioxidant activity of LFA was examined using the superoxide radical reduction assay in MAT-C cells. Total polyphenol contents (TPC) and flavonoid contents (TFC) of LFA were determined. The antibacterial activity of LFA against fish pathogens was also determined in this study. The viable cell number (9.38 log10 CFU/ml) and pH (4.1) results showed good adaptive ability of the selected strain during fermentation. LFA was found to have enhanced antioxidant activity compared to non-fermented A. annua L. (NFA) based on the DPPH assay. Cellular antioxidant activity was present in both LFA and NFA. After 24 hr and 48 hr of fermentation, the LFA also showed antibacterial activities against fish pathogens Photobacterium damselae subsp. damselae and Vibrio ichthyoenteri. These results suggest that L. plantarum-fermented A. annua L. may have potential as a feed additive in aquaculture.

주제어

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문제 정의

Up to date, few studies have reported the functional properties of fermented medicinal plants as a potential feed additive in aquaculture. Therefore, the objective of this study was to determine the in vitro antioxidant and antibacterial activities of fermented A. annua L. using an isolated Lactobacillus strain.

대상 데이터

A total of 13 strains were isolated and identified based on 16S RNA sequencing (Table 1). SK3493 and SK3494 showed high sequence similarities with lactic acid bacteria (LAB).
A. annua L. powder was purchased from a local market of Chengsong, Kyeongbuk, Korea. The plant was dried at 50℃ for 3 days and smashed into fine powder (150-mesh).
NFA showed no inhibitory effect on fish pathogen Photobacterium damselae subsp. damselae KCTC 2734. It had a slight inhibitory effect against Vibrio ichthyoenteri KCCM 40870 (Fig.

데이터처리

Data are presented as mean ± S.D. Small (pH) and capital (CFU) letters are significantly different by Duncan’s multiple range test at p<0.05.
Significant differences were determined using Duncan’s multiple range test at p<0.05 level.

이론/모형

Total flavonoids contents were estimated using the method described by Moreno [39] with slight modifications. Briefly, 0.
, Ltd, Seoul, Korea). Viable cell numbers were determined by drop plate method [17]. Supernatants were prepared by centrifugation (Mega 17R, Hanil science industrial, Incheon, South Korea) at 12,000 rpm, for 10 minutes at 4℃.
Antibacterial activities of NFA and LFA cultures against two fish pathogens Photobacterium damselae subsp. damselae ATCC 33539 and Vibrio ichthyoenteri ATCC 700023 were determined using agar well diffusion method [5]. Fish pathogens were pre-cultured in marine broth (MB) at 37℃ for 16 hr.

성능/효과

Antioxidant compounds might have been degraded during fermentation and some unknown compounds might have been synthesized during metabolism. In conclusion, Fermented A. annua L. showed antioxidant activity and antibacterial ability against fish pathogens, suggesting that it might be used as a potential feed additive in aquaculture.
Lactobacilli are well known for their antibacterial activity normally associated with organic acid and bacteriocin production [36]. In this study, LFA presented much higher antibacterial effect compared to NFA. This is possibly due to lactic acid production during fermentation.

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