ELISA has been used for the diagnosis of toxoplasmosis, but it is being gradually replaced by a rapid diagnostic test (RDT). We compared and analyzed ELISA and RDT results using the sera collected during 4 consecutive years from residents of Gyodong-do (Island), Incheon-city, Korea. Sera from 921, 9...
ELISA has been used for the diagnosis of toxoplasmosis, but it is being gradually replaced by a rapid diagnostic test (RDT). We compared and analyzed ELISA and RDT results using the sera collected during 4 consecutive years from residents of Gyodong-do (Island), Incheon-city, Korea. Sera from 921, 993, 940, and 838 adult residents were collected on a yearly basis (2010-2013). ELISA was performed by using a crude extract of T. gondii RH strain antigen and IgG/IgM RDT mounted with recombinant fragment of major surface antigen (SAG1), GST-linker-SAG1A, were applied to the sera. Comparison between groups was analyzed by the Student's t-test. The positive seroprevalence surged from 14.7% (135/921, 2010), 23.1% (231/993, 2011), 23.6% (222/940, 2012), and 32.1% (269/838, 2013) by ELISA. In contrast, RDT showed a more moderate increasing trend from 21.7% (200/921, 2010), 25.5% (253/993, 2011), 28.9% (272/940, 2012) and 33.1% (277/838, 2013). Discrepancies between ELISA and RDT were noted near the cut-off value. At the OD 0.15-0.24 range, RDT could detect 16.1% (169/1051) more positives, which suggests an early or acute toxoplasmosis, but at the OD 0.25-0.34 range, ELISA could detect 35.9% (92/256) more positives of possible chronic infections. Over the OD > 0.35 ELISA and RDT agreed in the majority of the cases. This surge in seroprevalence may be caused by the organic agriculture in addition to eating behavior or increase in pets among Koreans. These facts may be applied on a full-scale national survey using RDT to supplement ELISA to define the characteristics of the infection.
ELISA has been used for the diagnosis of toxoplasmosis, but it is being gradually replaced by a rapid diagnostic test (RDT). We compared and analyzed ELISA and RDT results using the sera collected during 4 consecutive years from residents of Gyodong-do (Island), Incheon-city, Korea. Sera from 921, 993, 940, and 838 adult residents were collected on a yearly basis (2010-2013). ELISA was performed by using a crude extract of T. gondii RH strain antigen and IgG/IgM RDT mounted with recombinant fragment of major surface antigen (SAG1), GST-linker-SAG1A, were applied to the sera. Comparison between groups was analyzed by the Student's t-test. The positive seroprevalence surged from 14.7% (135/921, 2010), 23.1% (231/993, 2011), 23.6% (222/940, 2012), and 32.1% (269/838, 2013) by ELISA. In contrast, RDT showed a more moderate increasing trend from 21.7% (200/921, 2010), 25.5% (253/993, 2011), 28.9% (272/940, 2012) and 33.1% (277/838, 2013). Discrepancies between ELISA and RDT were noted near the cut-off value. At the OD 0.15-0.24 range, RDT could detect 16.1% (169/1051) more positives, which suggests an early or acute toxoplasmosis, but at the OD 0.25-0.34 range, ELISA could detect 35.9% (92/256) more positives of possible chronic infections. Over the OD > 0.35 ELISA and RDT agreed in the majority of the cases. This surge in seroprevalence may be caused by the organic agriculture in addition to eating behavior or increase in pets among Koreans. These facts may be applied on a full-scale national survey using RDT to supplement ELISA to define the characteristics of the infection.
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제안 방법
1) were collected on a yearly basis from 2010 to 2013 (Table 1). Both ELISA and RDT were performed on the samples to counter-check each other.
대상 데이터
The current study compared and analyzed ELISA and RDT for the detection of anti-Toxoplasma antibodies with the sera of residents in Gyodong-do (Island), Incheon, Korea for 4 years from 2010 to 2013. An explosive increase in the seroprevalence was noted in the area.
데이터처리
Comparison between groups was analyzed by the Student’s t-test.
후속연구
In addition, the problems may not be limited to the island. Indeed, the proposed causes in the study may be applied to a full-scale national survey to screen the national toxoplasmic seroprevalence. In this respect, a national health policy would be required, and with a population of over 50 million in South Korea alone, a rapid and effective screening method will be helpful.
In addition, the problems may not be limited to the island. So, the postulated causes in this study may be applied to a full-scale national survey to screen the national toxoplasmic seroprevalence. Repeated surveys performed on a fixed time interval with more simple method such as RDT to supplement the ELISA is recommend- ed to define the characteristics of the infection as well as to find new infections.
참고문헌 (30)
1 Black MW Boothroyd JC Lytic cycle of Toxoplasma gondii Microbiol Mol Biol Rev 2000 64 607 623 10974128
10 Tsubota N Hiraoka K Sawada Y Watanabe T Ohshima S Studies on latex agglutination test for toxoplasmosis. 2. Evaluation the microtiter test as a serological test for toxoplasmosis in man Jpn J Parasitol 1977 26 286 290
11 Kobayashi A Hirai N Suzuki Y Nishikawa N Watanabe N Evaluaion of a commercial Toxoplasma latex agglutination test Jpn J Parasitol 1977 26 175 180
12 Choi WY Nam HW Youn JH Kim WS Kim WK Toxoplasma antibody titers by indirect latex agglutination test in patients of Kangnam St. Mary’s hospital and Cheju medical center Korean J Parasitol 1989 27 171 175
13 Choi WY Nam HW Youn JH Kim DJ Kong Y Kang SY Cho SY Detection of antibodies in serum and cerebrospinal fluid to Toxoplasma gondii by indirect latex agglutination test and enzyme-linked immunosorbent assay Korean J Parasitol 1992 30 83 90
14 Hong SJ Chong CK Lee K Kim TS Hong YP Ahn HJ Kim HY Ko AR Kim YJ Nam HW Maintained seroprevalence of toxoplasmosis among the residents of Jeju island, Korea Korean J Parasitol 2011 49 309 311 22072835
15 Yang HJ Jin KN Park YK Hong SC Bae JM Lee SH Choi HS Hwang HS Chung YB Lee NS Nam HW Seroprevalence of toxoplasmosis in the residents of Cheju island, Korea Korean J Parasitol 2000 38 91 93 10905070
16 Ahn HJ Cho PY Ahn SK Kim TS Chong CK Hong SJ Cha SH Nam HW Seroprevalence of toxoplasmosis in the residents of Cheorwon-gun, Gangwon-do, Korea Korean J Parasitol 2012 50 225 227 22949750
17 Choi WY Nam HW Yoo JE Toxoplasmacidal effect of HL-60 cells differentiated by dimethylsulfoxide Korean J Parasitol 1988 26 229 238
18 Sohn WM Nam HW Western blot analysis of stray cat sera against Toxoplasma gondii and the diagnostic availability of monoclonal antibodies in sandwich-ELISA Korean J Parasitol 1999 37 249 256 10634041
19 Song KJ Yang Z Chong CK Kim JS Lee KC Kim TS Nam HW A rapid diagnostic test for toxoplasmosis using recombinant antigenic N-terminal half of SAG1 linked with intrinsically unstructured domain of gra2 protein Korean J Parasitol 2013 51 503 510 24327774
20 Soh CT Lee SJ Ahn YG Latent infection by Toxoplasma gondii in Korea Yonsei Med J 1960 1 52 54
21 Dubey JP Hill DE Rozeboom DW Rajendran C Choudhary S Ferreira LR Kwok OC Su C High prevalence and genotypes of Toxoplasma gondii isolated from organic pigs in northern USA Vet Parasitol 2012 188 14 18 22483557
22 Meerburg BG De Craeye S Dierick K Kijlstra A Neospora caninum and Toxoplasma gondii in brain tissue of feral rodents and insectivores caught on farms in the Netherlands Vet Parasitol 2012 184 317 320 21958437
24 Kim DG Park JH Kim JL Jung BK Jeon SJ Lim H Lee MY Shin EH Klein TA Kim HC Chong ST Song JW Baek LJ Chai JY Intestinal nematodes from small mammals captured near the demilitarized zone, Gyeonggi province, Republic of Korea Korean J Parasitol 2015 53 135 139 25748722
25 André MR1 Adania CH Teixeira RH Silva KF Jusi MM Machado ST de Bortolli CP Falcade M Sousa L Alegretti SM Felippe PA Machado RZ Antibodies to Toxoplasma gondii and Neospora caninum in captive neotropical and exotic wild canids and felids J Parasitol 2010 96 1007 1009 20950109
26 McIntyre KM Setzkorn C Wardeh M Hepworth PJ Radford AD Baylis M Using open-access taxonomic and spatial information to create a comprehensive database for the study of mammalian and avian livestock and pet infections Prev Vet Med 2014 116 325 335 23906393
27 Tilahun G Tiao N Ferreira LR Choudhary S Oliveira S Verma SK Kwok OC Molla B Saville WJ Medhin G Kassa T Aleme H Gebreyes WA Su C Dubey JP Prevalence of Toxoplasma gondii from free-range chickens ( Gallus domesticus ) from Addis Ababa, Ethiopia J Parasitol 2013 99 740 741 23259902
28 Yang Z Cho PY Ahn SK Ahn HJ Kim TS Chong CK Hong SJ Cha SH Nam HW A surge in the seroprevalence of toxoplasmosis among the residents of islands in Gangwha-gun, Incheon, Korea Korean J Parasitol 2012 50 191 197 22949745
29 Kim JY Goo YK Zo YG Ji SY Trimarsanto H To S Clark TG Price RN Auburn S Further evidence of increasing diversity of Plasmodium vivax in the Republic of Korea in recent years PLoS One 2016 11 e0151514 26990869
30 Jeong YE Lee WC Cho JE Han MG Lee WJ Comparison of the epidemiological aspects of imported dengue cases between Korea and Japan, 2006–2010 Osong Public Health Res Perspect 2016 7 71 74 26981346
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