[논문]Immune Response to Koi Herpesvirus (KHV) of Koi and Koi × Red Common Carp (Cyprinus carpio)

Hwang, Ju-ae; Kim, Jung Eun; Kim, Hyeong-su; Lee, Jeong-Ho

doi:10.12717/dr.2017.21.4.361

[국내논문] Immune Response to Koi Herpesvirus (KHV) of Koi and Koi × Red Common Carp (Cyprinus carpio) 원문보기

Development & reproduction = 발생과 생식, v.21 no.4, 2017년, pp.361 - 370

Hwang, Ju-ae (Inland Aquaculture Research Center, National Institute of Fisheries Science (NIFS)) , Kim, Jung Eun (Inland Aquaculture Research Center, National Institute of Fisheries Science (NIFS)) , Kim, Hyeong-su (Inland Aquaculture Research Center, National Institute of Fisheries Science (NIFS)) , Lee, Jeong-Ho (Inland Aquaculture Research Center, National Institute of Fisheries Science (NIFS))

Abstract ▼ AI-Helper

Koi herpesvirus (KHV), also known as Cyprinid herpes virus 3 (Cyprinid 3) is lethal disease in common carp and koi (Cyprinus carpio). Two different groups (KK and RK) were infected KHV by intraperitoneal injection. Fish for gene expression analysis were sampled at 0 h, 12 h, 24 h, 48 h and 72 h post infection (p.i). The results showed that two immune related gene, Interferons (INFs) ${\alpha}{\beta}$ and Interleukin (IL)-12 p35 induced a high response in RK. The IL-12 p35 cytokine and Toll-like receptor (TLR) 9 were significantly high expressed on 48 h post infection (p.i) in RK as compared to the KK. The histopatological examination reveals focal necrosis in liver and infiltrate of lymphocytes in spleen of KK as compared to the RK. In immunohistochemistry analysis, the KHV protein high expressed in the infected kidney cell and slenocyte of KK. Therefore, the expression of IL-12 p35, IFN ${\alpha}{\beta}$ and TLR 9 may provide a potentially genes related with KHV resistance in Koi and red common carp ${\times}$ koi.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

제안 방법

Comparison of gene expression between groups and calibrator was derived from subtraction of the calibrator ΔCT values from the target ΔCT values to give a ΔΔCT value, and relative gene expression was calculated to determine fold difference (2–ΔΔCT).
Experiments were performed in triplicate aquaria with 3 groups: KK (koi × koi) and RK (red common carp × koi), and uninfected KK (control).
Expression of both genes was normalized to an endogenous reference β-actin and presented as subtraction of target CT values from β-actin CT values (ΔCT value).
sample from the controls were negative for KHV DNA. In this study, expression of immune-related genes were determined in kidneys collected from KK and RK during KHV infection. Target genes were standardized using reference gene β-actin (GeneBank accession no.
The expression pattern of the IFN ɑβ, IL-12 p35 and TLR 9 genes were investigated 12 h 24 h, 48 h and 72h using real time PCR.

대상 데이터

The KHV strain F347 (VR-1592) was purchased from American Type Culture Collection (ATCC). The virus was incubated in common carp brain (CCB) cells.
Two strains of koi and red common carp (Cyprinus carpio) were used in this study. The breeding for koi(Cyprinus carpio) and red common carp(Cyprinus carpio) was conducted at the Inland Aquaculture Research Center, National Institute of Fisheries Science, Changwon, Korea.

데이터처리

Comparison of gene expression between groups and calibrator was derived from subtraction of the calibrator ΔCT values from the target ΔCT values to give a ΔΔCT value, and relative gene expression was calculated to determine fold difference (2–ΔΔCT). The data were analyzed by oneway ANOVA using the SPSS statistical package (SPSS 5.0; SPSS Inc., USA). Means were separated using Duncan’s multiple range test and were considered different at P<0.

이론/모형

The gene specific primers are listed in Table 1. KHV DNA was detected using the CyHV-3-specific PCR procedure described by Gilas et al. (2002). DNA was extracted from gills using Qiagen DNeasy Blood & Tissue kit, and PCR amplification was performed using specific primers (Table.

참고문헌 (34)

1 Adamek M Rakus KL Chyb J Brogden G Huebner A Irnazarow I Steinhagen D Interferon type I responses to virus infections in carp cells: In Vitro studies on Cyprinid herpesvirus 3 and Rhabdovirus carpio infections Fish Shellfish Immunol 2012 33 482 493 10.1016/j.fsi.2012.05.031 22683518

상세보기
2 Adamek M Rakus KL Brogden G Matras M Chyb J Hirono I Kondo H Aoki T Irnazarow I Steinhagen D Interaction between type I interferon and Cyprinid herpesvirus 3 in different genetic lines of common carp (Cyprinus carpio L.) Dis Aquat Org 2014 111 107 118 10.3354/dao02773 25266898

상세보기
3 Adamek M Syakuri H Harris S Rakus KL Brogden G Matras M Irnazarow I Steinhagen D Cyprinid herpesvirus 3 infection disrupts the skin barrier of common carp (Cyprinus carpio) Vet Microbiol 2013 162 456 470 10.1016/j.vetmic.2012.10.033 23182910

상세보기
4 Aggad D Mazel M Boudinot P Mogensen KE Hamming OJ Hartmann R other authors The two groups of zebrafish virus-induced interferons signal via distinct receptors with specific and shared chains J Immunol 2009 183 3924 3931 10.4049/jimmunol.0901495 19717522

상세보기
5 Aoki T Hirono I Kurokawa K Fukuda H Nahary R Eldar A Genome sequences of three koi herpesvirus isolates representing the expending distribution of an emerging disease threatening koi and common carp worldwide J Virol 2007 81 5058 5065 10.1128/JVI.00146-07 PMC1900211 --> 17329333

상세보기
6 Bondat-Reantaso MG Sunarto A Subasinghe RP Managing the koi herpesvirus disease outbreak in Indonesia and the lessons learned Dev Biol (Basel) 2007 129 21 8 18306515
7 Dixon PF Joiner CL Way K Reese RA Jeney G Jeney Z Comparison of the resistance of selected families of a common carp, Cyprinus carpio L., to koi herpesvirus preliminary study J. Fish Dis 2009 32 1035 1039 10.1111/j.1365-2761.2009.01081.x 19602097

상세보기
8 Gilad O Yun S Andree KB Adkison MA Zlotkin A Bercovier H Eldar A Hedrick RP Initial characteristics of koi herpesvirus and development of a polymerase chain reaction assay to detect the virus in koi, Cyprinus carpio koi Dis Aquat Org 2002 48 101 108 10.3354/dao048101 12005231

상세보기
9 Gomez DK Joh SJ Jang H Shin SP Choresca CH Han JE Kim JH Jun JW Park SC Detection of koi herpesvirus (KHV) from koi (Cyprinus carpio koi) broodstock in South Korea Aquaculture 2011 311 42 47 10.1016/j.aquaculture.2010.11.021

상세보기
10 Hedrick RP Gilad O Yun S Spangenberg JV Marty GD Nordhausen RW Kebus MJ Bercovier H Eldar A A herpesvirus associated with mass mortality of juvenile and adult koi, a strain of a common carp J Aquat Anim Health 2000 12 44 57 10.1577/1548-8667(2000)012 2.0.CO;2 28880775

상세보기
11 Hedrick RP Waltzek TB McDowell TS Susceptibility of koi carp, common carp, goldfish and goldfish × common carp hybrids to cyprinid herpesvirus-2 and herpesvirus-3 J Aquat Anim Health 2006 18 26 34 10.1577/H05-028.1

상세보기
12 Hines RS Wohlfarth G Moav R Hulata G Genetic differences in susceptibility to two diseases among strains of common carp Aquaculture 1974 3 187 97 10.1016/0044-8486(74)90112-4

상세보기
13 Kongchum P Palti Y Hallerman EM Hulata G David L SNP discovery and development of genetic markers for mapping innate immune response genes in common carp (Cyprinus carpio) Fish & Shellfish Immunology 2010 29 356 361 10.1016/j.fsi.2010.04.013 20420915

상세보기
14 Kongchum P Sandel E Lutzky S Hallerman EM Hulata G David L Palti Y Association between IL-10a single nucleotide polymorphism and resistance to cyprinid herpesvirus-3 infection in common carp (Cyprinus carpio) J Aquacult 2011 315 417 421 10.1016/j.aquaculture.2011.02.035

상세보기
15 Li XS Liu ZZ Zhao XJ Ma XQ Feng W Wang CH Major histocompatibility complex DAB allele polymorphism and its association with resistance/ susceptibility to Aeromonas hydrophila in "whole red" color patterns of Cyprinus carpio var. color J Fish Sci China 2011 35 1293 1301
16 McGeoch DJ Rixon FR Davison AJ Topics in her-pesvirus genomics and evolution Virus Res 2006 117 90 104 10.1016/j.virusres.2006.01.002 16490275

상세보기
17 OIE Manual of Diagnostic Tests for Aquatic Animals 2013
18 Ouyang P Rakus K Boutier M Reschner A Leroy B Ronsmans M Fournier G Scohy S Costes B other au-thors The IL-10 homologue encoded by cypri-nid herpesvirus 3 is essential neither for viral replica-tion in vitro nor for virulence in vivo Vet Res 2013 1 44 53 10.1186/1297-9716-44-53

상세보기
19 Pikarsky E Ronen A Abramowitz J Levavi-Sivan B Pathogenesis of acute viral disease induced in fish by carp interstitial nephritis and gill necrosis virus J Virol 2004 78 9544 9551 10.1128/JVI.78.17.9544-9551.2004 PMC506927 --> 15308746

상세보기
20 Purcell MK LaPatra SE Woodson JC Kurath G Winton JR Early viral replication and induced or constitutive immunity in rainbow trout families with differential resistance to Infectious hematopoietic necrosis virus (IHNV) Fish Shellfish Immunol 2010 28 98 105 10.1016/j.fsi.2009.10.005 19819334

상세보기
21 Rakus KL Irnazarow I Adamek M Palmeira L Kawana Y Hirono I Kondo H Matras M Steinhagen D Flasz B Brogden G Vanderplasschen A Aoki T Gene expression analysis of common carp (Cyprinus carpio L.) lines during Cyprinid herpesvirus 3 infection yields insights into differential immune responses Dev Comp Immunol 2012 37 65 76 10.1016/j.dci.2011.12.006 22212509

상세보기
22 Rakus KŁ Wiegertjes GF Admek M Siwicki AK Lepa A Irnazaro I Resistance of common carp (Cypprinus carpio L.) to Cyprinid herpesvirus-3 is influenced by major histocompatibility (MH) class II B gene polymorphism Fish shellfish Immunol 2009 26 737 743 10.1016/j.fsi.2009.03.001 19328856

상세보기
23 Robertsen B The interferon system of teleost fish Fish Shellfish Immuol 2006 20 172 191 10.1016/j.fsi.2005.01.010 15939626

상세보기
24 Samuel CE Antiviral actions of interferons Clin Microbiol Rev 14 (4), pp 2001 10.1128/CMR.14.4.778-809.2001 PMC89003 --> 11585785

상세보기
25 Sano M Ito T Kurita J Yanai T Watanabe N Satoshi M et al. First detection of koi herpesvirus in cultured common carp Cyprinus carpio in Japan Fish Pathol 2004 39 165 167 10.3147/jsfp.39.165

상세보기
26 Shapira Y Magen Y Zak T Ktler M Hulata G Levavi-Sivan B Differential resistance to koi herpes virus (KHV)/carp interstitial nephritis and gill necrosis virus (CNGV) among common carp (Cyprinus carpio L.) strains and crossbreds Aquaculture 2005 245 1 11 10.1016/j.aquaculture.2004.11.038

상세보기
27 Sunarto A McColl KA Expression of immune-related genes of common carp during cyprinid herpesvirus 3 infection Dis Aquat Organ 2015 9 127 135 10.3354/dao02824 25751855

상세보기
28 Trinchieri G Interleukin-12 and the regulation of innate resistance and adaptive immunity Nat Rev Immunol 2003 3 133 146 10.1038/nri1001 12563297

상세보기
29 Tu C Weng MC Shiau JR Lin SY Detection of koi herpesvirus in koi Cyprinus carpio in Taiwan Fish Pathol 2004 39 109 110 10.3147/jsfp.39.109

상세보기
30 Verrier ER Langevin C Tohry C Houel A Genetic resistance to rhabdovirus infection in teleost fish is paralleled to the derived cell resistance status PLoS ONE 2012 7 e33935 10.1371/journal.pone.0033935 22514610

상세보기
31 Wang T Husain M The expanding repertoire of the IL-12 cytokine family in teleost fish: Identification of three paralogues each of the p35 and p40 genes in salmonids, and comparative analysis of their expression and modulation in Atlantic salmon Salmo salar Dev Comp Immunol 2014 46 194 207 10.1016/j.dci.2014.04.008 24759618

상세보기
32 Watford WT Moriguchi M Morinobu A O'Shea JJ The biology of IL-12: coordinating innate and adaptive immune responses Cytokine Growth Factor Rev 2003 14 361 368 10.1016/S1359-6101(03)00043-1 12948519

상세보기
33 Zhang S Wang Q Factors determining the formation and release of bioactive IL-12: regulatory mechanisms for IL-12p70 synthesis and inhibition Biochem Biophys Res Commun 2008 372 509 512 10.1016/j.bbrc.2008.05.081 18503756

상세보기
34 Zhao XJ Liu ZZ Li XS Wang Y Wang CH Major histocompatibility complex class II B allele polymorphism and its association with resistance/susceptibility to Aeromonas hydrophila in the "whole white" color pattern of Cyprinus carpio var. color J Fish Sci China 2012 19 399 407

LOADING...

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증