In a 12-week experiment, the rock bream, Oplegnathus fasciatus, and olive flounder, Paralichthys olivaceus, were investigated to determine the effects of starvation on their physiological parameters. The protein and DNA contents of the starved fish were significantly higher than the initial values a...
In a 12-week experiment, the rock bream, Oplegnathus fasciatus, and olive flounder, Paralichthys olivaceus, were investigated to determine the effects of starvation on their physiological parameters. The protein and DNA contents of the starved fish were significantly higher than the initial values and those of the fed fish. The RNA contents and RNA/DNA ratios of the fed fish were significantly higher than those of the other groups (P<0.05). The hematocrit, hemoglobin, red blood cells (RBC), and mean corpuscular volume (MCV) of the fed rock bream were significantly higher than at baseline (P<0.05), whereas the mean corpuscular hemoglobin concentration (MCHC) of the fed fish was lower than at baseline (P<0.05). The hematocrit, hemoglobin, RBC, and MCHC of the starved group were significantly lower than the baseline values, whereas the MCV of the starved group was significantly higher than the baseline value (P0.05). There were no significant differences in cortisol levels. However, the glucose level in the fed group was significantly higher than the baseline level and that in the starved group was significantly lower than the baseline level (P<0.05).
In a 12-week experiment, the rock bream, Oplegnathus fasciatus, and olive flounder, Paralichthys olivaceus, were investigated to determine the effects of starvation on their physiological parameters. The protein and DNA contents of the starved fish were significantly higher than the initial values and those of the fed fish. The RNA contents and RNA/DNA ratios of the fed fish were significantly higher than those of the other groups (P<0.05). The hematocrit, hemoglobin, red blood cells (RBC), and mean corpuscular volume (MCV) of the fed rock bream were significantly higher than at baseline (P<0.05), whereas the mean corpuscular hemoglobin concentration (MCHC) of the fed fish was lower than at baseline (P<0.05). The hematocrit, hemoglobin, RBC, and MCHC of the starved group were significantly lower than the baseline values, whereas the MCV of the starved group was significantly higher than the baseline value (P0.05). There were no significant differences in cortisol levels. However, the glucose level in the fed group was significantly higher than the baseline level and that in the starved group was significantly lower than the baseline level (P<0.05).
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문제 정의
The authors thank the technical staff of the Laboratory for Fishery Genetics and Breeding Science at Korea Maritime University, Korea, for their helpful support, and the anonymous reviewers who greatly improved the quality of this manuscript. All procedures used in this study complied with the current laws of korea (Ordinance of Agriculture, Food and Fisheries, no.
These experiments were undertaken to investigate physiology responses and DNA and RNA contents in the rock bream and olive flounder during starvation and feeding and to ascertain their growth and nutrition status. These experiments also provide information that should be useful for the aquaculture of both species.
제안 방법
Consequently, the relationship between RNA and DNA is an index of the cell’s metabolic intensity and has been shown to be sensitive to feeding levels (Bulow, 1970). To shed more light on this issue, we analyzed the effects of starvation on the physiological characteristics and RNA/DNA ratios of the rock bream and olive flounder.
대상 데이터
The rock bream, Oplegnathus fasciatus (standard length [SL] ± SD: 15.9 ± 0.76 cm; body weight [BW] ± SD:164.6 ± 32.21 g), and olive flounder, Paralichthys olivaceus (SL: 22.9 ± 0.88 cm; BW: 100.7 ± 11.67 g), were caught and transported to the Fishery Genetics and Breeding Science Laboratory of the Korea Maritime University, Korea, for the experiment.
데이터처리
The experiment was performed in triplicate and the results are reported as means ± standard deviations, unless otherwise stated. The data were analyzed with one-way ANOVA with the SPSS statistical package. The means were compared using Duncan’s multiple range test and considered significantly different at P<0.
이론/모형
(1951) and Ohnishi & Barr (1978). The RNA/DNA ratio was measured according to the methodology of Schmidt-Thannhauser-Schneider (Schneider 1957). The experiment was performed in triplicate and the results are reported as means ± standard deviations, unless otherwise stated.
성능/효과
In this study, our analysis of the rock bream, Oplegnathus fasciatus, and the olive flounder, Paralichthys olivaceus, showed that the protein contents of the starved groups increased. This suggests that the accumulation of fat in the livers of the fed fish caused a decline in liver cell content per unit liver weight.
No significant differences were observed in AST or ALT between the fed and initial groups (P>0.05), whereas only ALT of the starved fish was significantly higher than that of the initial test specimens (P<0.05).
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