Jung, Gi Hoon
(Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
,
Lim, Eun Seob
(Department of Food Biotechnology, Korea University of Science and Technology)
,
Woo, Min-Ah
(Division of Food Safety, Distribution and Standard, Korea Food Research Institute)
,
Lee, Joo Young
(Food Analysis Center, Korea Food Research Institute)
,
Kim, Joo-Sung
(Department of Food Biotechnology, Korea University of Science and Technology)
,
Paik, Hyun-Dong
(Department of Food Science and Biotechnology of Animal Resources, Konkuk University)
Campylobacter jejuni and Campylobacter coli are important foodborne pathogenic bacteria, particularly in poultry meat. In this study, the presence of extracellular DNase activity was investigated for biofilm-deficient Campylobacter strains versus biofilm-forming Campylobacter strains isolated from c...
Campylobacter jejuni and Campylobacter coli are important foodborne pathogenic bacteria, particularly in poultry meat. In this study, the presence of extracellular DNase activity was investigated for biofilm-deficient Campylobacter strains versus biofilm-forming Campylobacter strains isolated from chickens, to understand the relationship between extracellular DNase activity and biofilm formation. A biofilm-forming reference strain, C. jejuni NCTC11168, was co-incubated with biofilm non-forming strains isolated from raw chickens or their supernatants. The biofilm non-forming strains or supernatants significantly prohibited the biofilm formation of C. jejuni NCTC11168. In addition, the strains degraded pre-formed biofilms of C. jejuni NCTC11168. Degradation of C. jejuni NCTC11168 biofilm was confirmed after treatment with the supernatant of the biofilm non-forming strain 2-1 by confocal laser scanning microscopy. Quantitative analysis of the biofilm matrix revealed reduction of extracellular DNA (16%) and proteins (8.7%) after treatment. Whereas the biofilm-forming strains C. jejuni Y23-5 and C. coli 34-3 isolated from raw chickens and the C. jejuni NCTC11168 reference strain showed no extracellular DNase activity against their own genomic DNA, most biofilm non-forming strains tested, including C. jejuni 2-1, C. coli 34-1, and C. jejuni 63-1, exhibited obvious extracellular DNase activities against their own or 11168 genomic DNA, except for one biofilm non-former, C. jejuni 22-1. Our results suggest that extracellular DNase activity is a common feature suppressing biofilm formation among biofilm non-forming C. jejuni or C. coli strains of chicken origin.
Campylobacter jejuni and Campylobacter coli are important foodborne pathogenic bacteria, particularly in poultry meat. In this study, the presence of extracellular DNase activity was investigated for biofilm-deficient Campylobacter strains versus biofilm-forming Campylobacter strains isolated from chickens, to understand the relationship between extracellular DNase activity and biofilm formation. A biofilm-forming reference strain, C. jejuni NCTC11168, was co-incubated with biofilm non-forming strains isolated from raw chickens or their supernatants. The biofilm non-forming strains or supernatants significantly prohibited the biofilm formation of C. jejuni NCTC11168. In addition, the strains degraded pre-formed biofilms of C. jejuni NCTC11168. Degradation of C. jejuni NCTC11168 biofilm was confirmed after treatment with the supernatant of the biofilm non-forming strain 2-1 by confocal laser scanning microscopy. Quantitative analysis of the biofilm matrix revealed reduction of extracellular DNA (16%) and proteins (8.7%) after treatment. Whereas the biofilm-forming strains C. jejuni Y23-5 and C. coli 34-3 isolated from raw chickens and the C. jejuni NCTC11168 reference strain showed no extracellular DNase activity against their own genomic DNA, most biofilm non-forming strains tested, including C. jejuni 2-1, C. coli 34-1, and C. jejuni 63-1, exhibited obvious extracellular DNase activities against their own or 11168 genomic DNA, except for one biofilm non-former, C. jejuni 22-1. Our results suggest that extracellular DNase activity is a common feature suppressing biofilm formation among biofilm non-forming C. jejuni or C. coli strains of chicken origin.
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문제 정의
In addition, the role of extracellular DNase activity is poorly understood in the biofilm formation of the Campylobacter natural isolates. Therefore, this study was conducted to investigate the presence of extracellular DNase activity in biofilm-forming versus biofilm non-forming Campylobacter strains isolated from raw chicken meats, to understand the effects of this activity on biofilm formation by Campylobacter.
제안 방법
Biofilm-forming C. jejuni strain 11168 and each biofilm non-forming strain, C. jejuni 2-1, C. jejuni 22-1, C. coli 34-1, or C. jejuni 63-1, were mixed and incubated at 37°C for 72 h to investigate biofilm formation ability in different mixed cultures (Fig. 1).
Biofilm-forming strains C. jejuni 11168, C. jejuni Y23-5, and C. coli 34-3 were tested to investigate the presence of extracellular DNase activity against self-DNA (Fig. 8). The intensities of bands for strains 11168 and Y23-5 were nearly unchanged after 3 h incubation, while the intensities of bands for strain 34-3 showed slight decreases during incubation.
05). The measurements were normalized after the deduction of Mueller-Hinton broth only (negative control) for nonspecific binding. The data are based on three independent experiments conducted in triplicate.
Extracellular DNase activity plays an important role in biofilm modulation by acting on extracellular DNA [21, 22]. Thus, in this study, the extracellular DNase activity of biofilm non-forming strains was assessed by measuring the extent of genomic DNA degradation during incubation with the cells. First, genomic DNA of the standard strain 11168 was mixed with the cells of biofilm non-forming strains, and then the degradation of genomic DNA was assessed (Fig.
성능/효과
The intensities of bands for strains 11168 and Y23-5 were nearly unchanged after 3 h incubation, while the intensities of bands for strain 34-3 showed slight decreases during incubation. These data suggest that the biofilm-forming strains did not degrade their own genomic DNA or only slightly degraded genomic DNA during 3 h incubation, revealing the absence of, or very weak, extracellular DNase activity (Fig. 8).
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