[국내논문]Japanese Hard Ticks (Ixodes nipponensis) Parasitizing on the Endangered Leopard Cat (Prionailurus bengalensis euptilura) in the Republic of Korea원문보기
Because of the potential negative influence on their hosts, ecto-parasites are of prime importance to numerous species. Ticks are among these, distributed worldwide, and potentially transmitting diseases while sucking blood of diverse hosts. The leopard cat (Prionailurus bengalensis euptilura Elliot...
Because of the potential negative influence on their hosts, ecto-parasites are of prime importance to numerous species. Ticks are among these, distributed worldwide, and potentially transmitting diseases while sucking blood of diverse hosts. The leopard cat (Prionailurus bengalensis euptilura Elliot, 1871) is the only felid left in the Republic of Korea following widespread anthropogenic disturbances that have resulted in the extinction of both Panthera species: the Siberian tiger(Panthera tigris altaica Temminck, 1844) and Amur leopard (P. pardus orientalis(Schlegel, 1857)). This study identifies ticks collected from a roadkill leopard cat retrieved in Seosan area in the Republic of Korea. Two ticks attached to the facial area of the carcass were identified as Japanese hard ticks, Ixodes nipponensis, based on mitochondrial cytochrome oxidase I. The matching sample was from Japan with 99.7% similarities, and the only available sequence on GenBank. This study reconfirms that I. nipponensis parasitizes the endangered leopard cat P. bengalensis euptilura.
Because of the potential negative influence on their hosts, ecto-parasites are of prime importance to numerous species. Ticks are among these, distributed worldwide, and potentially transmitting diseases while sucking blood of diverse hosts. The leopard cat (Prionailurus bengalensis euptilura Elliot, 1871) is the only felid left in the Republic of Korea following widespread anthropogenic disturbances that have resulted in the extinction of both Panthera species: the Siberian tiger(Panthera tigris altaica Temminck, 1844) and Amur leopard (P. pardus orientalis(Schlegel, 1857)). This study identifies ticks collected from a roadkill leopard cat retrieved in Seosan area in the Republic of Korea. Two ticks attached to the facial area of the carcass were identified as Japanese hard ticks, Ixodes nipponensis, based on mitochondrial cytochrome oxidase I. The matching sample was from Japan with 99.7% similarities, and the only available sequence on GenBank. This study reconfirms that I. nipponensis parasitizes the endangered leopard cat P. bengalensis euptilura.
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제안 방법
04, Biomatters Limited, Auckland, New Zealand). Finally, we analyzed the genetic variations between the sequences obtained and the matching sequences from GenBank through BLAST searches (https://blast.ncbi. nlm.nih.gov/Blast.cgi) with n related sequences.
We used a touchdown PCR protocol with a range of 52 to 46℃, with minus two degrees increment for the annealing temperature. The protocol was set as follows: initial denaturation (94℃, 5 min); followed by five cycles of 94℃ for 30 s, 52℃ for 30 s, and 68℃ for 1 min; five cycles of 94℃ for 30 s, 50℃ for 30 s, and 68℃ for 1 min; five cycles of 94℃ for 30 s, 48℃ for 30 s, and 68℃ for 1 min; 25 cycles of 94℃ for 30 s, 46℃ for 30 s, and 68℃ for 1 min; finally followed by a final extension step of 68℃ for 5 min. PCRs were carried on a SimpliAmp Thermal Cycler (Applied Biosystems by Life Technologies, Carlsbad, CA, USA).
대상 데이터
asanumai has so far been confirmed in Japan but never in Korea. Both sequences were uploaded on GenBank under accession number: KY606283 and KY606284. Comparison between the two 768 bp COI sequences obtained and the matching Japanese I.
The collection was conducted in Aejeong-ri, Seosan-si, South Chungcheong Province, 36.72372°N, 126.409916°E, on 20 Nov 2016.
이론/모형
KK was supported by a BK scholarship and the work was done under the financial support of the National Geographic Society and the Rural Development Administration (PJ 012285) to YJ. The collection and experiment in this study comply with the current laws of the Republic of Korea (Ministry of Environment Permit Numbers: Guem river area 2017-10).
성능/효과
Both sequences were uploaded on GenBank under accession number: KY606283 and KY606284. Comparison between the two 768 bp COI sequences obtained and the matching Japanese I. nipponensis from Genbank revealed high conservativeness. There were only three nucleotides differences at the positions 357, 570 and 654.
The ticks collected from the leopard cat were identified as Ixodes nipponensis from both molecular and morphological examinations. First, the BLAST search for the sequences obtained from the two ticks was 99.7% matching with the only record of I. nipponensis. Ixodes asanumai Kitaoka, 1973 also showed high matching result of 98.
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