[논문]Whitening Activity of Abeliophyllum distichum Nakai Leaves According to the Ratio of Prethanol A in the Extracts

Jang, Tae-Won; Choi, Ji-Soo; Kim, Hoi-Ki; Lee, Eun-Ja; Han, Man-Wook; Lee, Ki-Beom; Kim, Do-Wan; Park, Jae-Ho

doi:10.7732/kjpr.2018.31.6.667

Whitening Activity of Abeliophyllum distichum Nakai Leaves According to the Ratio of Prethanol A in the Extracts 원문보기

韓國資源植物學會誌 = Korean journal of plant resources, v.31 no.6, 2018년, pp.667 - 674

Jang, Tae-Won (Department of Medicinal Plant Resources, Andong National University) , Choi, Ji-Soo (Department of Medicinal Plant Science, Jungwon University) , Kim, Hoi-Ki (Fanipinkorea Co., Ltd) , Lee, Eun-Ja (Fanipinkorea Co., Ltd) , Han, Man-Wook (Fanipinkorea Co., Ltd) , Lee, Ki-Beom (Incheon Business Information Technopark. Biotechnology & Business Center) , Kim, Do-Wan (Department of Food Science and Industry, Jungwon University) , Park, Jae-Ho (Department of Pharmaceutical Science, Jungwon University)

Abstract ▼ AI-Helper

In this study, we evaluated the whitening activity of prethanol A and water extracts from Abeliophyllum distichum Nakai. The extracts were prepared using 0, 50, 70, and 100% prethanol A at $121^{\circ}C$, 1.2 atm for 15 minutes. To confirm effective extraction, the acteoside content of each extract was analyzed with the HPLC-PDA method. The antioxidant activity was evaluated using DPPH and ABTS scavenging activity assays, and the whitening activity was evaluated based on inhibitory activities on the protein and mRNA expression of tyrosinase, tyrosinase-related protein 1 (TRP-1), tyrosinase-related protein 2 (TRP-2), and microphthalmia-associated transcription factor (MITF) in B16 F10 cells. Each extract showed strong antioxidant and whitening activity. $IC_{50}$ values of antioxidant activity from each extract were in order of 100%, 70%, 50%, and 0%. In addition, whitening activity inhibited the protein and mRNA expression of melanin synthesis factor, following the same pattern as antioxidant activity. In conclusion, water and prethanol A extracts of A. distichum showed effective antioxidant and whitening activity and are thus considered to be valuable materials for whitening cosmetics. The results of this study will also provide basic data for the safe and efficient production of A. distichum as a cosmetic material.

주제어

표/그림 (5)

그림 Fig. 1. Acteoside chromatograms from Abeliophyllum distichum Nakai leaves according to the ratio of prethanol A in the extracts by HPLC-PDA.
그림 Fig. 2. Reducing power of Abeliophyllum distichum Nakai leaves according to the ratio of prethanol A in the extracts.
표 Table 1. DPPH and ABTS radical scavenging activity of Abeliophyllum distichum Nakai leaves according to the ratio of prethanol A in the extracts
그림 Fig. 3. Whitening activities on melanin synthesis related proteins of Abeliophyllum distichum Nakai leaves according to the ratio of prethanol A in the extracts.
그림 Fig. 4. Whitening activities on melanin synthesis related mRNA levels of Abeliophyllum distichum Nakai leaves according to the ratio of prethanol A in the extracts.

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제안 방법

PCR was carried out using Quick Taq® HS DyeMix (Toyobo) with primers for Tyrosinase forward 5’-GAG AAG CGA GTC TTG ATT AG-3’, reverse 5’-TGG TGC TTC ATG GGC AAA ATC-3’, TRP-1 forward 5’-GCT GCA GGA GCC TTC TTT CTC-3’, reverse 5’-AAG ACG CTG CAC TGC TGG TCT-3’, TRP-2 forward 5’-CCT GTC TCT CCA GAA GTT TG-3’, reverse 5’-CGT CTG TAA AAG AGT GGA GG-3’, MITF forward 5’-AGC GTG TAT TTT CCC CAC AG-3’, reverse 5’-TAG CTC CTT AAT GCG GTC GT-3’, GADPH forward 5'-AAC TTT GGC 223 ATT GTG GAA GG-3', reverse 5'-ATG CAG GGA TGA TGT TCT GG-3'.

대상 데이터

distichum Nakai (voucher number: JWU-031) was collected from Misun-hyang Theme park, Seongbul-Mountain Recreation Forest, 78, Chungmin-rogigok-gil, Goesan-eup, Goesan-gun, Chungcheongbuk-do, Korea. Acetonitrile, chloroform, dimethyl sulfoxide, ethyl acetate, methanol, petroleum ether and prethanol A (HPLC-grade) were purchased from Merck (Darmstadt, Germany). Dulbecco’s modified Eagle’s medium (DMEM), 10% (v/v) fetal bovine serum, penicillin/streptomycin, and trypsin were purchased from Hyclone (Logan, UT, USA).
Louis, MO, USA), unless otherwise specified. Standard acteoside was purchased from Sigma-Aldrich.

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