The polyherbal drug Gyeongshingangjeehwan 18 (GGEx18) from Rheum palmatum L. (Polygonaceae), Laminaria japonica Aresch (Laminariaceae), and Ephedra sinica Stapf (Ephedraceae) has traditionally been used as an antiobesity drug in Korean local clinics. This study investigates the effects of GGEx18 on ...
The polyherbal drug Gyeongshingangjeehwan 18 (GGEx18) from Rheum palmatum L. (Polygonaceae), Laminaria japonica Aresch (Laminariaceae), and Ephedra sinica Stapf (Ephedraceae) has traditionally been used as an antiobesity drug in Korean local clinics. This study investigates the effects of GGEx18 on pancreatic fibroinflammation in high-fat diet (HFD)-fed obese C57BL/6J mice and the molecular mechanism involved in this process. After HFD-fed obese C57BL/6J mice were treated with GGEx18 (125, 250, and 500 mg/kg) for 12 weeks, variables and determinants of obesity, pancreatic inflammation, and fibrosis were measured using histology, immunohistochemistry, and real-time polymerase chain reaction. Administration of GGEx18 at 500 mg/kg/day to obese mice decreased body weight gain, mesenteric adipose tissue mass, and adipocyte size. GGEx18 treatment not only reduced mast cells and CD68-immunoreactive cells, but also decreased collagen levels and ${\alpha}$-smooth muscle actin-positive cells in the pancreas of HFD-fed mice. Concomitantly, GGEx18 decreased the expression of genes for inflammation (i.e., CD68 and tumor necrosis factor ${\alpha}$) and fibrosis (i.e., collagen ${\alpha}1$ and transforming growth factor ${\beta}$) in the pancreas of obese mice. These results suggest that GGEx18 may inhibit visceral obesity and related pancreatic fibroinflammation in HFD-fed obese mice.
The polyherbal drug Gyeongshingangjeehwan 18 (GGEx18) from Rheum palmatum L. (Polygonaceae), Laminaria japonica Aresch (Laminariaceae), and Ephedra sinica Stapf (Ephedraceae) has traditionally been used as an antiobesity drug in Korean local clinics. This study investigates the effects of GGEx18 on pancreatic fibroinflammation in high-fat diet (HFD)-fed obese C57BL/6J mice and the molecular mechanism involved in this process. After HFD-fed obese C57BL/6J mice were treated with GGEx18 (125, 250, and 500 mg/kg) for 12 weeks, variables and determinants of obesity, pancreatic inflammation, and fibrosis were measured using histology, immunohistochemistry, and real-time polymerase chain reaction. Administration of GGEx18 at 500 mg/kg/day to obese mice decreased body weight gain, mesenteric adipose tissue mass, and adipocyte size. GGEx18 treatment not only reduced mast cells and CD68-immunoreactive cells, but also decreased collagen levels and ${\alpha}$-smooth muscle actin-positive cells in the pancreas of HFD-fed mice. Concomitantly, GGEx18 decreased the expression of genes for inflammation (i.e., CD68 and tumor necrosis factor ${\alpha}$) and fibrosis (i.e., collagen ${\alpha}1$ and transforming growth factor ${\beta}$) in the pancreas of obese mice. These results suggest that GGEx18 may inhibit visceral obesity and related pancreatic fibroinflammation in HFD-fed obese mice.
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제안 방법
In this study, we treated HFD-induced obese mice with GGEx18 for 12 weeks and assessed the effects on body weight gain, visceral mesenteric adipose tissue mass, adipocyte size, pancreatic inflammation and fibrosis, and expression of pancreatic genes involved in inflammation and fibrosis. Our results suggest that GGEx18 inhibits pancreatic fibroinflammation by reducing inflammatory cell infiltration, collagen accumulation, and pancreatic expression of fibroinflammation-related genes.
대상 데이터
Eight-week-old male wild-type C57BL/6J mice (n=8/ group) were purchased from Central Lab Animal (Seoul, Korea) and randomly divided into five groups. Mice were fed a low-fat diet (LFD; 13% kcal fat, CJ, Incheon, Korea), a HFD (45% kcal fat, Research Diets, New Brunswick, NJ, USA) or the HFD supplemented with one of three doses of GGEx18 (HFD-GGEx18; 125, 250, or 500 mg/kg/day) for 12 weeks.
The genes of interest were amplified from the synthesized cDNA using AccuPower® GreenStarTM qPCR PreMix (Bioneer, Deajeon, Korea) on an ExcyclerTM 96 Real Time Quantitative Thermal Block machine (Bioneer).
The percent composition of each ingredient is equal to that currently used to treat human patients. Voucher specimens for Ephedra sinica (FOS-05-04), Laminaria japonica (FOS-05-05), and Rheum palmatum (FOS-05-06) were deposited at the Department of Formula Sciences, Dongeui University. Briefly, three dried herbs with their contents weighted were boiled together in distilled water for 22 h at 95℃.
데이터처리
Statistical analysis was performed by ANOVA followed by either Tukey's multiple comparison or Dunnett's post hoc tests.
성능/효과
In conclusion, these studies demonstrate that GGEx18 decreases the expression of genes involved in inflammation and fibrosis in the pancreas of obese mice. These changes led to decreased inflammatory cell infiltration and collagen accumulation, thereby reducing pancreatic inflammation and fibrosis.
Our results show that GGEx18 treatment for 12 weeks decreased visceral fat mass and inhibited visceral adipocyte hypertrophy in HFD-fed mice. Of three doses of GGEx18, GGEx18 at the dose of 500 mg/kg/day significantly reduced body weight gain and mesenteric adipose tissue weight in HFD-fed mice.
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