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식물의 감수분열에서 상동 재조합 효소 특이 기능의 연구현황 및 전망
Current status and prospects of the meiosis-specific function of recombinase in plants 원문보기

Journal of plant biotechnology = 식물생명공학회지, v.45 no.1, 2018년, pp.1 - 8  

정유진 (국립한경대학교 원예생명과학과) ,  남기홍 (국립한경대학교 원예생명과학과) ,  김태성 (한국방송통신대학교 농학과) ,  이인혜 (국립생물자원관 생물소재연구단 연구팀) ,  조용구 (충북대학교 식물자원학과) ,  강권규 (국립한경대학교 원예생명과학과)

Abstract AI-Helper 아이콘AI-Helper

Meiosis is a specialized cell division, essential in most reproducing organisms to halve the number of chromosomes, thereby enabling the restoration of ploidy levels during fertilization. A key step in meiosis is homologous recombination, which promotes homologous pairing and generates crossovers (C...

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가설 설정

  • The tripartite complex finds a DNA sequence homologous to single-stranded DNA from the template strand, an intermediate for homologous chain search. (D) Homologous regions are found, heteroduplex DNA regions are formed. At this time, the DNA strand is also separated.
본문요약 정보가 도움이 되었나요?

질의응답

핵심어 질문 논문에서 추출한 답변
세포는 무엇인가? 세포는 생물의 기본 단위이며, 그 유전 정보는 게놈 DNA에 기록되어 있다. 유성생식을 통해 세대진전을 하는 생물의 세포는 다음 세대에 유전 정보를 전달하는 역할을 하는 생식세포와 체세포로 대별 된다.
감수 제 1 분열기는 몇 단계로 나눌 수 있는 가? 1 B). 감수 제 1 분열기는 염색체의 구조 변화에 따라, Lepton stage,Zigoten stage, Pakité Stage, Diploten stage, Deakinesis stage 등 다섯 단계로 나눌 수 있다(Fraune et al., 2012).
감수분열기의 세포가 RAD51 이외에 DMC1도 기능하는 것을 통해서 무엇을 예상할 수 있는 가? 감수분열기의 세포는 RAD51 이외에 DMC1도 기능한다. 따라서 감수분열기 재조합은 RAD51과 DMC1의 양자가 협력하여 기능하는 것을 생각할 수 있다. 유전학적 해석으로부터 DMC1은 감수분열기 재조합에 필수이며, 중심적인 역할을 담당하고 있는 것으로 알려져 있지만, RAD51의 역할에 대해서는 아직 불분명 한 점이 많다.
질의응답 정보가 도움이 되었나요?

참고문헌 (59)

  1. Aboussekhra A, Chanet R, Adjiri A, Fabre F (1992) Semidominant suppressors of Srs2 helicase mutations of Saccharomyces cerevisiae map in the RAD51 gene, whose sequence predicts a protein with similarities to procaryotic RecA proteins. Mol Cell Biol 12:3224-3234 

  2. Aihara H, Ito Y, Kurumizaka H, Yokoyama S, Shibata T (1999) The N-terminal domain of the human Rad51 protein binds DNA: structure and a DNA binding surface as revealed by NMR. J Mol Biol 290:495-504 

  3. Allers T, Lichten M (2001) Differential timing and control of noncrossover and crossover recombination during meiosis. Cell 106:47-57 

  4. Basile G, Aker M, Mortimer RK (1992) Nucleotide sequence and transcriptional regulation of the yeast recombinational repair gene RAD51. Mol Cell Biol 12:3235-3246 

  5. Baumann P, Benson FE, West SC (1996) Human Rad51 protein promotes ATP-dependent homologous pairing and strand transfer reactions in vitro. Cell 87:757-766 

  6. Benson FE, Stasiak A, West SC (1994) Purification and characterization of the human Rad51 protein, an analogue of E. coli RecA. EMBO J 13:5764-5771 

  7. Bishop DK, Park D, Xu L, Kleckner N (1992) DMC1: a meiosisspecific yeast homolog of E. coli recA required for recombination, synaptonemal complex formation, and cell cycle progression. Cell 69:439-456 

  8. Bugreev DV, Golub EI, Stasiak AZ, Stasiak A, Mazin AV (2005) Activation of human meiosis-specific recombinase Dmc1 by Ca2+. J Biol Chem 280:26886-22695 

  9. Bugreev DV, Pezza RJ, Mazina OM, Voloshin ON, Camerini-Otero RD, Mazin AV (2011) The resistance of DMC1 D-loops to dissociation may account for the DMC1 requirement in meiosis. Nat Struct Mol Biol 18:56-60 

  10. Busygina V, Sehorn MG, Shi IY, Tsubouchi H, Roeder GS, Sung P (2008) Hed1 regulates Rad51-mediated recombination via a novel mechanism. Genes Dev 22:786-795 

  11. Chen J, Villanueva N, Rould MA, Morrical SW (2010) Insights into the mechanism of Rad51 recombinase from the structure and properties of a filament interface mutant. Nucleic Acids Res. 38:4889-4906 

  12. Chen Z, Yang H, Pavletich NP (2008) Mechanism of homologous recombination from the RecA-ssDNA/dsDNA structures. Nature 453:489-494 

  13. Cloud V, Chan YL, Grubb J, Budke B, Bishop DK (2012) Rad51 is an accessory factor for Dmc1-mediated joint molecule formation during meiosis. Science 337:1222-1225 

  14. Cole F, Kauppi L, Lange J, Roig I, Wang R, Keeney S, Jasin M (2012) Homeostatic control of recombination is implemented progressively in mouse meiosis. Nat Cell Biol 4:424-430 

  15. Conway AB, Lynch TW, Zhang Y, Fortin GS, Fung CW, Symington LS, Rice PA (2004) Crystal structure of a Rad51 filament. Nat Struct Mol Biol 11:791-796 

  16. Da Ines O, Degroote F, Goubely C, Amiard S, Gallego ME, White CI (2013) Meiotic recombination in Arabidopsis is catalysed by DMC1, with RAD51 playing a supporting role. PLoS Genet 9, e1003787 

  17. Fraune J, Schramm S, Alsheimer M, Benavente R (2012) The mammalian synaptonemal complex: protein components, assembly and role in meiotic recombination. Exp Cell Res 318:1340-1346 

  18. Gupta RC, Bazemore LR, Golub EI, Radding CM (1997) Activities of human recombination protein Rad51. Proc Natl Acad Sci USA 94:463-468 

  19. Habu T, Taki T, West A, Nishimune Y, Morita T (1996) The mouse and human homologs of DMC1, the yeast meiosis-specific homologous recombination gene, have a common unique form of exon-skipped transcript in meiosis. Nucleic Acids Res 24:470-477 

  20. Hikiba J, Hirota K, Kagawa W, Ikawa S, Kinebuchi T, Sakane I, Takizawa Y, Yokoyama S, Mandon-Pepin B, Nicolas A, Shibata T, Ohta K, Kurumizaka H (2008) Structural and functional analyses of the DMC1-M200V polymorphism found in the human population. Nucleic Acids Res 36:4181-4190 

  21. Kagawa W, Kurumizaka H (2010). From meiosis to postmeiotic events: uncovering the molecular roles of the meiosis-specific recombinase Dmc1. FEBS J 277:590-598 

  22. Keeney S (2008) Spo11 and the Formation of DNA Double-Strand Breaks in Meiosis. Genome Dyn Stab 2:81-123 

  23. Keeney S, Giroux CN, Kleckner N (1997) Meiosis-specific DNA double-strand breaks are catalyzed by Spo11, a member of a widely conserved protein family. Cell 88:375-384 

  24. Kurumizaka H, Aihara H, Ikawa S, Kashima T, Bazemore L R, Kawasaki K, Sarai A, Radding C M, Shibata T (1996) A possible role of the C-terminal domain of the RecA protein. J Biol Chem 271:33515-33524 

  25. Kurumizaka H, Ikawa S, Sarai A, Shibata T (1999) The mutant RecA proteins, RecAR243Q and RecAK245N, exhibit defective DNA binding in homologous pairing. Arch Biochem Biophys 365:83-91 

  26. Lim DS, Hasty PA (1996) Mutation in mouse rad51 results in an early embryonic lethal that is suppressed by a mutation in p53. Mol Cell Biol 16:7133-7143 

  27. Liu Y, Gaines WA, Callender T, Busygina V, Oke A, Sung P, Fung JC, Hollingsworth NM (2014) Down-regulation of Rad51 activity during meiosis in yeast prevents competition with Dmc1 for repair of double-strand breaks. PLoS Genet 10:e1004005 

  28. Maeshima K, Morimatsu K, Horii T (1996) Purification and characterization of XRad51.1 protein, Xenopus RAD51 homologue: recombinant XRad51.1 promotes strand exchange reaction. Genes Cells 1:1057-1068 

  29. Mandon-Pepin B, Derbois C, Matsuda F, Cotinot C, Wolgemuth DJ, Smith K, McElreavey K, Nicolas A, Fellous M (2002) Human infertility: meiotic genes as potential candidates. Gynecol. Obstet Fertil. 30:817-821 

  30. Mandon-Pepin B, Touraine P, Kuttenn F, Derbois C, Rouxel A, Matsuda F, Nicolas A, Cotinot C, Fellous M (2008) Genetic investigation of four meiotic genes in women with premature ovarian failure. European J Endocrinol 158:07-115 

  31. Masson JY, Davies AA, Hajibagheri N, Van Dyck, E, Benson FE, Stasiak AZ, Stasiak A, West SC (1999) The meiosis-specific recombinase hDmc1 forms ring structures and interacts with hRad51. EMBO J 8:6552-6560 

  32. Matsuo Y, Sakane I, Takizawa Y, Takahashi M, Kurumizaka H (2006) Roles of the human Rad51 L1 and L2 loops in DNA binding. FEBS J 273:3148-3159 

  33. Mimitou EP, Symington LS (2008). Sae2, Exo1 and Sgs1 collaborate in DNA double-strand break processing. Nature 455:770-774 

  34. Nassif N, Penney J, Pal S, Engels WR, Gloor GB (1994) Efficient copying of nonhomologous sequences from ectopic sites via P-element-induced gap repair. Mol Cell Biol 14:1613-1625 

  35. Neale MJ, Keeney S (2006). Clarifying the mechanics of DNA strand exchange in meiotic recombination. Nature 442:, 153-158 

  36. Niu H, Wan L, Busygina V, Kwon Y, Allen JA, Li X, Kunz RC, Kubota K, Wang B, Sung P, Shokat KM, Gygi SP, Hollingsworth NM (2009) Regulation of meiotic recombination via Mek1-mediated Rad54 phosphorylation. Mol Cell 36:393-404 

  37. Ogawa T, Yu X, Shinohara A, Egelman EH (1993) Similarity of the yeast RAD51 filament to the bacterial RecA filament. Science 259:1896-1899 

  38. Passy SI, Yu X, Li Z, Radding CM, Masson JY, West SC, Egelman EH (1999) Human Dmc1 protein binds DNA as an octameric ring. Proc Natl Acad Sci USA 96:10684-10688 

  39. Petronczki M, Siomos MF, Nasmyth K (2003) Un menage a quatre: the molecular biology of chromosome segregation in meiosis. Cell 112:423-40 

  40. Pittman DL, Cobb J, Schimenti KJ, Wilson LA, Cooper DM, Brignull E, Handel MA, Schimenti JC (1998) Meiotic prophase arrest with failure of chromosome synapsis in mice deficient for Dmc1, a germline-specific RecA homolog. Mol Cell 1:697-705 

  41. Renkawitz J, Lademann C A, Jentsch S (2014) Mechanisms and principles of homology search during recombination. Nat Rev. Mol Cell Biol 15:369-383 

  42. Reymer A, Frykholm K, Morimatsu K, Takahashi M, Norden B (2009) Structure of human Rad51 protein filament from molecular modeling and site-specific linear dichroism spectroscopy. Proc Natl Acad Sci USA 106:13248-53 

  43. Roeder GS (1997) Meiotic chromosomes: it takes two to tango. Genes Dev 15:2600-2621 

  44. Sakane I, Kamataki C, Takizawa Y, Nakashima M, Toki S, Ichikawa H, Ikawa S, Shibata T, Kurumizaka H (2008) Filament formation and robust strand exchange activities of the rice DMC1A and DMC1B proteins. Nucleic Acids Res 6:4266-4276 

  45. San Filippo J, Sung P, Klein H (2008) Mechanism of eukaryotic homologous recombination. Annu Rev Biochem 77: 229-257 

  46. Schwacha A, Kleckner N (1997) Interhomolog bias during meiotic recombination: meiotic functions promote a highly differentiated interhomolog-only pathway. Cell 190:1123-1135 

  47. Sehorn MG, Sigurdsson S, Bussen W, Unger VM, Sung P (2004) Human meiotic recombinase Dmc1 promotes ATP-dependent homologous DNA strand exchange. Nature 429:433-437 

  48. Shinohara A, Ogawa H, Ogawa T (1992) Rad51 protein involved in repair and recombination in S. cerevisiae is a RecA-like protein. Cell 69:457-470 

  49. Shinohara A, Ogawa H, Matsuda Y, Ushio N, Ikeo K, Ogawa T (1993) Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA. Nat Genet 4:239-243 

  50. Shinohara M, Oh SD, Hunter N, Shinohara A (2008) Crossover assurance and crossover interference are distinctly regulated by the ZMM proteins during yeast meiosis. Nat Genet 40:299-309 

  51. Sonoda E, Sasaki MS, Buerstedde JM, Bezzubova O, Shinohara A, Ogawa H, Takata M, Yamaguchi-Iwai Y, Takeda S (1998) Rad51-deficient vertebrate cells accumulate chromosomal breaks prior to cell death. EMBO J 17:598-608 

  52. Sung P (1994) Catalysis of ATP-dependent homologous DNA pairing and strand exchange by yeast RAD51 protein. Science 265:1241-1243 

  53. Symington L S (2002) Role of RAD52 epistasis group genes in homologous recombination and double-strand break repair. Microbiol Mol Biol Rev 66:630-670 

  54. Szostak JW, Orr-Weaver TL, Rothstein RJ, Stahl FW (1983) The double-strand-break repair model for recombination Cell 33: 25-35 

  55. Tsuzuki T, Fujii Y, Sakumi K, Tominaga Y, Nakao K, Sekiguchi M, Matsushiro A, Yoshimura Y, Morita T (1996) Targeted disruption of the Rad51 gene leads to lethality in embryonic mice. Proc Natl Acad Sci USA 93:6236-6240 

  56. West SC (2003) Molecular views of recombination proteins and their control. Nat Rev Mol Cell Biol 4:435-445 

  57. Yoshida K, Kondoh G, Matsuda Y, Habu T, Nishimune Y, Morita T (1998) The mouse RecA-like gene Dmc1 is required for homologous chromosome synapsis during meiosis. Mol Cell 1:707-718 

  58. Yu X, Jacobs SA, West SC, Ogawa T, Egelman EH (2001) Domain structure and dynamics in the helical filaments formed by RecA and Rad51 on DNA. Proc Natl Acad Sci USA 98:8419-8424 

  59. Zhou Y, Caron P, Legube G, Paull TT (2014) Quantitation of DNA double-strand break resection intermediates in human cells. Nucleic Acids Res 42:e19 

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