사이퍼메트린은 피레스로이드 계열 살충제로서 오랫동안 농업과 가정에서 이용되어 왔으며 그들의 잔여 성분과 독성에 대한 경각심이 고취되고 있다. 부산 인근의 해안에서 분리된 Cellulophaga lytica DAU203 균주가 사이퍼메트린의 생물학적 분해 활성을 나타내었다. DAU203 균주는 최소 배지에서 유일 탄소원으로 사이퍼메트린을 첨가하였을 때, 이를 분해하여 탄소원으로 활용 하였다. 반응표면분석법을 통하여 DAU203 균주의 사이퍼메트린 분해를 위한 최적 조건을 탐색하였다. 온도, pH와 yeast extract 첨가 농도와 같은 인자가 분해 활성에 영향을 미치는 것으로 분석 되었고, 각각의 최적 값은 $33.4^{\circ}C$, pH 7.7와 2.4%(w/v)이다. 최적 조건에서 DAU203 균주는 5일 동안 대략 83.7%의 사이퍼메트린을 분해하였다. 본 연구는 미생물 중에서 잘 연구되지 않은 해양 미생물에 대한 활용 가능성을 증가시켰다고 판단된다.
사이퍼메트린은 피레스로이드 계열 살충제로서 오랫동안 농업과 가정에서 이용되어 왔으며 그들의 잔여 성분과 독성에 대한 경각심이 고취되고 있다. 부산 인근의 해안에서 분리된 Cellulophaga lytica DAU203 균주가 사이퍼메트린의 생물학적 분해 활성을 나타내었다. DAU203 균주는 최소 배지에서 유일 탄소원으로 사이퍼메트린을 첨가하였을 때, 이를 분해하여 탄소원으로 활용 하였다. 반응표면분석법을 통하여 DAU203 균주의 사이퍼메트린 분해를 위한 최적 조건을 탐색하였다. 온도, pH와 yeast extract 첨가 농도와 같은 인자가 분해 활성에 영향을 미치는 것으로 분석 되었고, 각각의 최적 값은 $33.4^{\circ}C$, pH 7.7와 2.4%(w/v)이다. 최적 조건에서 DAU203 균주는 5일 동안 대략 83.7%의 사이퍼메트린을 분해하였다. 본 연구는 미생물 중에서 잘 연구되지 않은 해양 미생물에 대한 활용 가능성을 증가시켰다고 판단된다.
Cypermethrin, a commonly used domestic and agricultural pyrethroid pesticide, is widely considered detrimental to the environment and to many organisms because of its residual property and toxicity. Cellulophaga lytica DAU203, isolated from coastal sediment, was chosen because it degrade cypermethri...
Cypermethrin, a commonly used domestic and agricultural pyrethroid pesticide, is widely considered detrimental to the environment and to many organisms because of its residual property and toxicity. Cellulophaga lytica DAU203, isolated from coastal sediment, was chosen because it degrade cypermethrin. Cellulophaga lytica DAU203 effectively degraded cypermethrin, as the utilized carbon source and substrate, in a mineral salt medium. Effective factors, such as carbon source, nitrogen source, initial pH, and temperature, for cypermethtin biological degradation by Cellulophaga lytica DAU203 were analyzed by one factor at a time method. Temperature ($22{\sim}42^{\circ}C$), initial pH (5~9), and yeast extract concentration (0.1~2.5%[w/v]) were selected as the three most important factors. There were optimized at $33.4^{\circ}C$, pH 7.7, and 2.4%(w/v) by response surface methodology, respectively. The Box- Behnken design consisting of 46 experimental runs with three replicates was used to optimize the independent variables which significantly influenced the cypermethrin biological degradation. This model for cypermethrin degradation by Cellulophaga lytica DAU203 is highly significant (p<0.05). Under the optimized condition, Cellulophaga lytica DAU203 degraded approximately 83.7 % of the cypermethrin within 5 days. These results suggest that Cellulophaga lytica DAU203 may be useful for the biological degradation of cypermethrin in cypermethrin-contaminated environments.
Cypermethrin, a commonly used domestic and agricultural pyrethroid pesticide, is widely considered detrimental to the environment and to many organisms because of its residual property and toxicity. Cellulophaga lytica DAU203, isolated from coastal sediment, was chosen because it degrade cypermethrin. Cellulophaga lytica DAU203 effectively degraded cypermethrin, as the utilized carbon source and substrate, in a mineral salt medium. Effective factors, such as carbon source, nitrogen source, initial pH, and temperature, for cypermethtin biological degradation by Cellulophaga lytica DAU203 were analyzed by one factor at a time method. Temperature ($22{\sim}42^{\circ}C$), initial pH (5~9), and yeast extract concentration (0.1~2.5%[w/v]) were selected as the three most important factors. There were optimized at $33.4^{\circ}C$, pH 7.7, and 2.4%(w/v) by response surface methodology, respectively. The Box- Behnken design consisting of 46 experimental runs with three replicates was used to optimize the independent variables which significantly influenced the cypermethrin biological degradation. This model for cypermethrin degradation by Cellulophaga lytica DAU203 is highly significant (p<0.05). Under the optimized condition, Cellulophaga lytica DAU203 degraded approximately 83.7 % of the cypermethrin within 5 days. These results suggest that Cellulophaga lytica DAU203 may be useful for the biological degradation of cypermethrin in cypermethrin-contaminated environments.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
제안 방법
A variety of factors were identified as the main variables by preliminary experiments, and the range of independent variables were determined as follows: yeast extract concentration (0.1-2.5%, [w/v]), temperature (22-42℃), and pH (5-9). RSM based on the Box-Behnken design was used to investigate the inter-variable interactions that markedly affected the degradation of cypermethrin by DAU203.
The pH and temperature factors were known as important factor to degrade cypermethrin biologically [3]. In this experiment, using the Box-Behnken design, the degradation of cypermethrin was evaluated and the effect of combined experimental design as a dependent variable was shown (Table. 1). These results were analyzed by the statistical software Design-Expert (Ver.
, Minneapolis, MN, USA). Regression analysis was performed using the conditions obtained from the program, with each experiment performed in triplicate. The dependent variable was the degradation ratio of 50 mg/l cypermethrin in MSM after 5 days of culture by DAU203.
5%(w/v), such as ammonium nitrate, casamino acids, casein, peptone, skim milk, tryptone, urea, and yeast extract, were prepared to determine the best source for cypermethrin biodegradation. The medium was incubated at 30℃ in 250 ml flasks with 50 mg/l cypermethrin on a rotary shaker, and experiments were performed in triplicate, using non-inoculated samples as controls. After 5 days, the cypermethrin concentration of the samples was determined by HPLC.
이론/모형
lytica DAU203, from coastal sediment. Optimization of the biological degradation of cypermethrin by C. lytica DAU203 was performed using response surface methodology (RSM) based on the Box-Behnken design. The isolated bacterium might be useful for the bioremediation of cypermethrin-contaminated coastal sediment and soil.
성능/효과
05 suggested statistical significance at >95% confidence level. The determination coefficient (R2=0.9494) indicated approximately 94.94% of the variability in response, demonstrating that the predicted values of the model were in perfect agreement with the experimental values. The high value of the adjusted R2 (0.
The regression analysis indicated that X1 and X2X3 played significant roles (p<0.05), while other terms had no significant effects on cypermethrin degradation (p>0.05).
참고문헌 (13)
Chen, S. H., Dong, Y. H., Chang, C. Q., Deng, Y. Y., Zhang, X. F., Zhong, G. H., Song, H. W., Hu, M. Y. and Zhang, L. H. 2013. Characterization of a novel cyfluthrin-degrading bacterial strain Brevibacterium aureum and its biochemical degradation pathway. Bioresour. Technol. 132, 16-23.
Chen, S. H., Hu, M. Y., Liu, J. J., Zhong, G. H., Yang, L., Rizwan-ul-Haq, M. and Han, H. T. 2011. Biodegradation of beta-cypermethrin and 3-phenoxybenzoic acid by a novel Ochrobactrum lupini DG-S-01. J. Hazard Mater. 187, 433-440.
Chen, S. H., Luo, J. J., Hu, M. Y., Lai, K. P., Geng, P. and Huang, H. S. 2012. Enhancement of cypermethrin degradation by a coculture of Bacillus cereus ZH-3 and Streptomyces aureus HP-S-01. Bioresour. Technol. 110, 97-104.
Guo, P., Wang, B. Z., Hang, B. J., Li, L., Ali, S. W., He, J. and Li, S. P. 2009. Pyrethroid-degrading Sphingobium sp. JZ-2 and the purification and characterization of a novel pyrethroid hydrolase. Int. Biodeterior. Biodegr. 63, 1107-1112.
Kuivila, K. M., Hladik, M. L., Ingersoll, C. G., Kemble, N. E., Moran, P. W., Calhoun, D. L., Nowell, L. H. and Giliom, R. J. 2012. Occurrence and potential sources of pyrethroid insecticides in stream sediments from seven U.S. metropolitan areas. Environ. Sci. Technol. 46, 4297-4303.
Lao, W., Tiefenthaler, L., Greenstein, D. J., Maruya, K. A., Bay, S. M., Ritter, K. and Schiff, K. 2012. Pyrethroids in Southern California coastal sediments. Environ. Toxicol. Chem. 31, 1649-1656.
Shafer, T. J., Meyer, D. A. and Crofton, K. M. 2005. Developmental neurotoxicity of pyrethroid insecticides: critical review and future research needs. Environ. Health Perspect. 113, 123-136.
Soderlund, D. M., Clark, J. M., Sheets, L. P., Mullin, L. S., Piccirillo, V. J., Sargent, D., Stevens, J. T. and Weiner, M. L. 2002. Mechanisms of pyrethroid neurotoxicity: implications for cumulative risk assessment. Toxicology 171, 3-59.
Weston, D. P., Holmes, R. W. and Lydy, M. J. 2009. Residential runoff as a source of pyrethroid pesticides to urban creeks. Environ. Pollut. 157, 287-294.
Wolansky, M. J. and Harrill, J. A. 2008. Neurobehavioral toxicology of pyrethroid insecticides in adult animals: a critical review. Neurotoxicol. Teratol. 30, 55-78.
Zhang, C., Jia, L., Wang, S. H., Qu, J., Li, K., Xu, L. L., Shi, Y. H. and Yan, Y. C. 2010. Biodegradation of beta-cypermethrin by two Serratia spp. with different cell surface hydrophobicity. Bioresour. Technol. 101, 3423-3429.
Zhang, C., Wang, S. H. and Yan, Y. C. 2011. Isomerization and biodegradation of beta-cypermethrin by Pseudomonas aeruginosa CH7 with biosurfactant production. Bioresour. Technol. 102, 7139-7146.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.