[논문]Anti-Obesity Effects of a Mixture of Fermented Ginseng, Bifidobacterium longum BORI, and Lactobacillus paracasei CH88 in High-Fat Diet-Fed Mice

Kang, Dayoung; Li, Zhipeng; Ji, Geun Eog

doi:10.4014/jmb.1801.01016

Anti-Obesity Effects of a Mixture of Fermented Ginseng, Bifidobacterium longum BORI, and Lactobacillus paracasei CH88 in High-Fat Diet-Fed Mice 원문보기

Journal of microbiology and biotechnology, v.28 no.5, 2018년, pp.688 - 696

Kang, Dayoung (Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University) , Li, Zhipeng (Department of Biomedical Science, College of Veterinary Medicine, Oregon State University) , Ji, Geun Eog (Department of Food and Nutrition, Research Institute of Human Ecology, Seoul National University)

Abstract ▼ AI-Helper

Ginseng and probiotics have anti-obesity effects in mice fed a high-fat diet (HFD). Absorption of ginsenoside and colonization of probiotics occur in the intestine. In this study, a mixture of fermented ginseng and two probiotics, Bifidobacterium longum BORI and Lactobacillus paracasei CH88, was administered to HFD-fed mice for 9 weeks. The mixture significantly suppressed weight gain (p < 0.05, n = 8) and lipid deposition in the liver and adipose tissues as well as increased the mice's food intake. The adipocyte size of the adipose tissue was significantly decreased in the mixture-fed group, especially when 0.5% fermented ginseng and $5{\times}10^8/ml$ of the two probiotics were used (p < 0.05, n = 10). The expression of TNF-${\alpha}$ in adipose tissue was efficiently downregulated in the mixture-fed group (p < 0.05, n = 4). The supplement also improved the mice's fasting blood glucose levels (p < 0.05, n = 8) and total cholesterol feces excretion (p < 0.05, n = 8). The mixture of fermented ginseng and B. longum BORI and L. paracasei CH88 could have an anti-obesity effect and suppress lipid deposit in the liver and adipose tissues.

주제어

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문제 정의

Therefore, the purpose of this study was to evaluate whether a FG and probiotics mixture had an anti-obesity effect on mice fed a HFD.

제안 방법

After a 1-week adaptation period, the mice were randomly divided into the following five groups (n = 8): LFD (Low-fat diet; 10% fat of total calories), HFD (High-fat diet; 60% fat of total calories), or HFD supplemented with FG and PM (FG+PM) divided into LOW (0.25% FG + 2.5 × 108 CFU/ml PM), MID (0.5% FG + 5 × 108 CFU/ml PM), and HIGH(1.0% FG + 1 × 109 CFU/ml PM).

대상 데이터

The flow rate and temperature were set to 1 ml/min and 20°C, respectively. Ginsenosides Rb1, Rb2, Rd, Rg1, and F1 were purchased from Biotech (China). Ginsenosides Rc, Re, Rg2, Rg3, Rh1, Rh2, and cK were obtained from Cogon Biotech (China).
The main ginsenosides of the experimental FG were protopanaxadiol-type Rd (19.1%), Rc (4.5%), and protopanaxatriol-type Re (3.3%). The others were Rb1 performed to measure the hepatic fat accumulation and adipocyte (1.

데이터처리

The results were analyzed using the SPSS statistical package ANOVA test. The statistical differences were examined via Duncan’s multiple range test.
The statistical differences were examined via Duncan’s multiple range test.

성능/효과

Obesity is associated with insulin resistance, and elevated FBG changes lipogenesis and lipolysis. According to the results of this study, although the FBG level was increased in the HFD group, the FG+PM level was lower and effectively inhibited in the MID group. This is consistent with the results that ginseng [15] and probiotics [19] decreased blood sugar.
Ginseng and probiotics are well known as NAFLD-ameliorating agents. The results of this study show that the liver weight and hepatic TAG content were effectively suppressed in the FG+PM groups, especially in the MID group. Moreover, the smallest lipid droplet size was observed in the MID group (Fig.

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