Simultaneous Determination of Four Compounds from Artemisia capillaris using High Performance Liquid Chromatography-Ultraviolet Detector (HPLC-UVD) and Their Quantitative Study in Artemisia Genus원문보기
Artemisia capillaris Thunb. (Compositae) is a native herb of East Asian countries and has used for the treatment of jaundice, high liver fever, and digestive diseases for a long time, as well as being developed as the source of herbal preparations until now. The major components from A. capillaris w...
Artemisia capillaris Thunb. (Compositae) is a native herb of East Asian countries and has used for the treatment of jaundice, high liver fever, and digestive diseases for a long time, as well as being developed as the source of herbal preparations until now. The major components from A. capillaris were chlorogenic acid (1) and its derivatives substituted with caffeoyl moieties, such as 3,5-dicaffeoylquinic acid (2) and 4,5-dicaffeoylquinic acid (3), and coumarins, such as scoparone. In the study, four compounds, chlorogenic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid and scoparone (4) in the 70% ethanolic extract of A. capillaris were simultaneously determined by using HPLC-UVD system. This method was validated with the terms of linearity, precious and accuracy according to ICH guidelines. The developed method was successfully applied for the quantitative analysis of Artemisia genus, A. capillaris, A. iwayomogi, A. princeps, and A. argyi, distributed in Korea.
Artemisia capillaris Thunb. (Compositae) is a native herb of East Asian countries and has used for the treatment of jaundice, high liver fever, and digestive diseases for a long time, as well as being developed as the source of herbal preparations until now. The major components from A. capillaris were chlorogenic acid (1) and its derivatives substituted with caffeoyl moieties, such as 3,5-dicaffeoylquinic acid (2) and 4,5-dicaffeoylquinic acid (3), and coumarins, such as scoparone. In the study, four compounds, chlorogenic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid and scoparone (4) in the 70% ethanolic extract of A. capillaris were simultaneously determined by using HPLC-UVD system. This method was validated with the terms of linearity, precious and accuracy according to ICH guidelines. The developed method was successfully applied for the quantitative analysis of Artemisia genus, A. capillaris, A. iwayomogi, A. princeps, and A. argyi, distributed in Korea.
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제안 방법
HPLC analysis was conducted on a HECTOR C18-M column (M51002546, 4.6 × 250 mm, i.d., 4.5-μm pore size).
In this study, four compounds, CA, 3,5-DQA, 4,5-DQA and SP, in the 70% ethanolic extract of A. capillaris were simultaneously determined using HPLC-UV system and validated in terms of specificity, linearity, precision and accuracy. The validated method was successfully applied to quantify all four compounds in the Artemisia samples distributed in Korean traditional markets.
Quantification of Four Standards in Artemisia genus Samples − Thirty-one Artemisia samples distributed from Korea traditional herb markets were applied to quantify four standards, CA, 3,5-DQA, 4,5-DQA and SP under the analytical conditions developed in the method validation study.
The relative standard deviation (RSD) was taken as a measure of repeatability. Then, recovery test was used to investigate the accuracy of this analysis method. The standard solutions with three different concentrations (100, 200 and 300 ppm) were added to the 70% ethanolic extract of A.
Three different columns, HECTOR C18-M column (4.6 ×250 mm, i.d., 4.5 μm), YMC-Triart C18 (4.6 × 250 mm, i.d., 5 μm) and Hypersil GOLD (4.6 × 250 mm, i.d., 5 μm), were applied to the HPLC-UV system, but a HECTOR C18-M column showed better performance for the separation of four standards than others and used to the further analyses.
대상 데이터
Plant Materials − Thirty-one dried whole herbs of Artemisia samples, 5 of A. capillary, 16 of A. iwayomogi, 8 of A. princeps, and 2 of A. argyi, were provided from Prof. Young Ho Kim, a professor of College of Pharmacy, Chungnam National University, identified by Dr. Yong Soo Kwon, a professor of College of Pharmacy, Kangwon National University.
Yong Soo Kwon, a professor of College of Pharmacy, Kangwon National University. The samples were deposited in the Herbarium of College of Pharmacy, Kangwon National University (KNUPH-AC-1~5 for A. capillaris, KNUPH-AI-1~16 for A. iwayomogi, KNUPH-AP-1~8 for A. princeps and KNUPH-AA-1~2 for A. argyi).
이론/모형
Limit of detection (LOD) and quantification (LOQ) were determined using signal-to-noise ratios (S/N) of 3 and 10, respectively. For the precision results, intra- and inter-day test were performed to determine the precision of the developed HPLC analytical method. The relative standard deviation (RSD) was taken as a measure of repeatability.
성능/효과
Quantification of Four Standards in the Dried Herbs of Artemisia genus −The contents of four standards, CA, 3,5-DQA, 4,5-DQA and SP, were measured from thirtyone samples of Artemisia genus, A. capillaris (5 samples), A. iwayomogi (16 samples), A. princeps (8 samples) and A. argyi (2 samples) under the developed analytical condition.
The precision of the developed method was verified by intra- and interday variations, and it was expressed by the relative standard deviation (RSD). The RSD values of intra- and inter-day were less than 4.49% and 6.10%, respectively, which exhibited a good precision of the developed method (Table 2). To verify the accuracy, the standard solutions with three different concentrations were spiked to the 70% ethanolic extract of A.
참고문헌 (12)
Jang, E.; Kim, B. J.; Lee, K. T.; Inn, K. S.; Lee, J. H. Evid. Based Complement. Alternat. Med. 2015, 2015, 728137.
Park, K. M.; Li, Y.; Kim, B.; Zhang, H.; Hwangbo, K.; Piao, D. G.; Chi, M. J.; Woo, M. H.; Choi, J. S.; Lee, J. H.; Moon, D. C.; Chang, H. W.; Kim, J. R.; Son, J. K. Arch. Pharm. Res. 2012, 35, 2153-2162.
Jang, S. I.; Kim, Y. J.; Lee, W. Y.; Kwak, K. C.; Baek, S. H.; Kwak, G. B.; Yun, Y. G.; Kwon, T. O.; Chung, H. T.; Chai, K. Y. Arch. Pharm. Res. 2005, 28, 203-208.
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