[논문]New polymorphic microsatellite markers for the endangered fern Ceratopteris thalictroides (Parkeriaceae)

CHO, Won-Bum; HAN, Eun-Kyeong; KWAK, Myounghai; LEE, Jung-Hyun

doi:10.11110/kjpt.2018.48.2.129

New polymorphic microsatellite markers for the endangered fern Ceratopteris thalictroides (Parkeriaceae) 원문보기

식물분류학회지 = Korean journal of plant taxonomy, v.48 no.2, 2018년, pp.129 - 133

CHO, Won-Bum (Department of Biology Education, Chonnam National University) , HAN, Eun-Kyeong (Department of Biology Education, Chonnam National University) , KWAK, Myounghai (Plant Resources Division, National Institute of Biological Resources) , LEE, Jung-Hyun (Department of Biology Education, Chonnam National University)

Abstract ▼ AI-Helper

Ceratopteris thalictroides is a semi-aquatic fern with a circumtropical distribution. Because this species is designated internationally on the IUCN Red List as requiring at least some concern, Korean populations are of great concern for the species' long-term survival, as they are at the northern limit of the species distribution. To establish an effective conservation strategy for those populations at the genetic level, we used the Mi-Seq platform to develop three sets of 25 polymorphic microsatellite markers for C. thalictroides, which is endangered in Korea. In populations sampled from Busan and Gochang, the number of alleles ranged from 2 to 13 (average of 5.64), and plants presented an expected heterozygosity of 0.000 to 0.860. These markers will be useful for evaluating the genetic status and conserving Korean populations of C. thalictroides more effectively.

주제어

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제안 방법

5 min at 72℃; with a final extension for 10 min at 72℃. The PCR products were analyzed using an ABI 3730XL sequencer with GeneScan-500LIZ Size Standard (Applied Biosystems, Foster City, CA, USA). Allele sizes and the peaks of each sample were determined via Peak Scanner software 2 (Applied Biosystems).

대상 데이터

To test the effectiveness of these new microsatellite loci, we sampled 63 individuals of C. thalictroides from two populations (Dongnim reservoir, Gochang, n = 33; Is. Doonchido, Busan, n = 30). For each locus, PCR amplification was performed in a final volume of 5 µL that contained 15 to 20 ng of extracted DNA, 2.

이론/모형

The extraction procedure involved an MG Plant Genomic DNA Extraction SV Miniprep Kit used according to the manufacturer’s protocol (Macrogene Inc., Seongnam, Korea).

참고문헌 (13)

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상세보기
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상세보기
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상세보기
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