식물정유 및 혼합물의 점박이응애(Tetranychus urticae Koch)에 대한 살비 및 기피활성 Toxicity and Repellent Activity of Plant Essential Oils and Their Blending Effects Against Two Spotted Spider Mites, Tetranychus urticae Koch원문보기
본 연구에서는, 20종의 식물정유를 이용하여 점박이응애 성충에 대한 살비활성 및 기피활성을 확인하였다. 살비활성 평가에서는 샌달우드 오일이, 기피활성 평가에서는 클라리 세이지 오일이 가장 높은 효과를 나타내었고, 평가에 사용한 20종의 식물정유간에는 살비활성과 기피활성간의 상관관계가 매우 낮게 나타났다. 높은 활성을 나타낸 정유들의 혼합시험에서는, 거의 대부분의 조합이 서로간에 저해효과를 갖는 것으로 확인되었다. 샌달우드 및 클라리 세이지 오일의 구성성분은 GC/MS 분석을 통해 확인하였으며, 샌달우드는 세스퀴터펜류가, 클라리 세이지 오일은 모노터펜류가 주종을 이루었다. 클라리세이지 오일의 구성성분 중에서는 linalyl acetate가 가장 높은 함량을 갖고 있을 뿐만 아니라, 해당정유가 기피효과를 갖는 주된 효능물질임을 확인하였다.
본 연구에서는, 20종의 식물정유를 이용하여 점박이응애 성충에 대한 살비활성 및 기피활성을 확인하였다. 살비활성 평가에서는 샌달우드 오일이, 기피활성 평가에서는 클라리 세이지 오일이 가장 높은 효과를 나타내었고, 평가에 사용한 20종의 식물정유간에는 살비활성과 기피활성간의 상관관계가 매우 낮게 나타났다. 높은 활성을 나타낸 정유들의 혼합시험에서는, 거의 대부분의 조합이 서로간에 저해효과를 갖는 것으로 확인되었다. 샌달우드 및 클라리 세이지 오일의 구성성분은 GC/MS 분석을 통해 확인하였으며, 샌달우드는 세스퀴터펜류가, 클라리 세이지 오일은 모노터펜류가 주종을 이루었다. 클라리세이지 오일의 구성성분 중에서는 linalyl acetate가 가장 높은 함량을 갖고 있을 뿐만 아니라, 해당정유가 기피효과를 갖는 주된 효능물질임을 확인하였다.
Miticidal and repellent activity of twenty plant essential oils against the adults of two spotted spider mites, Tetranychus urticae, were examined. Sandal wood oil was the most potent one in mortality, whereas clary sage oil exhibited the greatest repellent activity. On those twenty essential oils t...
Miticidal and repellent activity of twenty plant essential oils against the adults of two spotted spider mites, Tetranychus urticae, were examined. Sandal wood oil was the most potent one in mortality, whereas clary sage oil exhibited the greatest repellent activity. On those twenty essential oils tested, no apparent correlation between toxicity and repellency was observed. When the most active oils were blended, the combinations tend to exhibit antagonistic interactions in both toxicity and repellent activity. The chemical compositions of sandal wood oil and clary sage oil were identified via GC/MS analyses, and the major constituents of sandal wood oils were sesquiterpene compounds, whereas the major ones for clary sage oil were monoterpenes. Among the major components in clary sage oil, linalyl acetate was not only the most abundant constituent, but also the most responsible one for its repellent activity.
Miticidal and repellent activity of twenty plant essential oils against the adults of two spotted spider mites, Tetranychus urticae, were examined. Sandal wood oil was the most potent one in mortality, whereas clary sage oil exhibited the greatest repellent activity. On those twenty essential oils tested, no apparent correlation between toxicity and repellency was observed. When the most active oils were blended, the combinations tend to exhibit antagonistic interactions in both toxicity and repellent activity. The chemical compositions of sandal wood oil and clary sage oil were identified via GC/MS analyses, and the major constituents of sandal wood oils were sesquiterpene compounds, whereas the major ones for clary sage oil were monoterpenes. Among the major components in clary sage oil, linalyl acetate was not only the most abundant constituent, but also the most responsible one for its repellent activity.
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제안 방법
0 µL, and the initial temperature for the oven was set at 50℃ for 5 min, then increased to 65℃, 120℃, 180℃, 210℃ and 325℃ with each rate of 10℃/min, 50℃/min, 5℃/min, 50℃/min and 20℃/min, respectively. Each stage was held for 30 min, 10 min, 10 min, 0 min, 10 min and 10 min. The total runtime was 101.
0 mL/min. The data were analyzed using NIST Mass Spectral Search software (version 2.0), and the major constituents were determined by matching the spectra against the NIST/EPA/NIH Mass Spectral Library.
To examine the mixture effect of the plant essential oils, binary mixtures of sandal wood oil (which was the most toxic) and five other oils (which showed the greatest repellent activity) were prepared at two different doses [(5 mg + 5 mg)/ml and (10 mg + 10 mg)/ml]. The same bioassay methods were employed to each of the toxicity and repellent activity test.
대상 데이터
Twenty plant essential oils (organic grades) were purchased from Klimtech (Dimitrovgrad, Bulgaria), and their scientific names and origins are listed in Table 1. The individual monoterpene compounds in the present study were obtained from Sigma-Aldrich (Darmstadt, Germany; linalool, a-terpineol, geraniol, a-terpinyl acetate, geranyl acetate) or Tokyo Chemical Industry CO.
데이터처리
ANOVA test was conducted to compare the miticidal and repellent activity of the test oils as well as the comparison among the major constituents of clary sage oil, and Student T-test was performed for the correlation analysis between the two activity by using a SPSS software (version 2.5) for the statistical analyses.
성능/효과
Rather, all of the five combinations were determined to pose antagonistic interactions, as showing significantly less toxicity compared to the expected (calculated) mortality. Among the combinations tested, the mixture of sandal wood and clary sage oil revealed the most notable decrease in the toxicity (X2 value of 30.7), and the lower concentration (5 + 5 mg/mL each) failed to show any mortality.
후속연구
urticae, the contribution of the minor constituents also cannot be neglected. A further study on more comprehensive understanding in the role of those minor constituents in clary sage oil is required to fully understand the contribution of each compound to the overall repellent activity.
Lastly, the main active constituent for clary sage oil's repellent activity was identified as linalyl acetate, with synergetic interactions with minor constituents. The studies about veiled mechanisms in phenomena of synergetic and antagonistic interactions between essential oil constituents will further be needed.
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