Park, Jong Youn
(South Sea Fisheries Research Institute, National Institute of Fisheries Science)
,
Cho, Jae Kwon
(South Sea Fisheries Research Institute, National Institute of Fisheries Science)
,
Choi, Young Jae
(South Sea Fisheries Research Institute, National Institute of Fisheries Science)
,
Han, Kyeong Ho
(Dept. of Aqualige Science, Chonnam National University)
,
Hong, Chang Gi
(South Sea Fisheries Research Institute, National Institute of Fisheries Science)
We determined the morphologic characteristics (body weight and degree of abdomen inflation) of the red spotted grouper, Epinephelus akaara, mother fish producing healthy eggs. Experimental fish were chosen from fish reared in a sea cage. The fish were divided into four size groups by body weight: 40...
We determined the morphologic characteristics (body weight and degree of abdomen inflation) of the red spotted grouper, Epinephelus akaara, mother fish producing healthy eggs. Experimental fish were chosen from fish reared in a sea cage. The fish were divided into four size groups by body weight: 400~600, 600~800, 800~1,000, and 1,000~1,200 g and four stages (I~IV) of the degree of abdomen inflation. After hormone treatment, we observed the amount of ovulation-induced eggs, and rates of buoyancy, fertilization, embryonic survival, and hatching. As a result, mother fish with a body weight of 600 g or more spawned, and the fertilization rate, embryonic survival rate, and hatching rate were high in the 800~1,000 g range, thus showing effective ovulation induction. As a result of dividing the degree of abdomen inflation based on the anal fin of the mother fish into I-IV stages and determining hormone treatment time, the GSI was $0.9{\pm}0.2%$ at stage I, $2.3{\pm}0.2%$ at stage II, $5.6{\pm0.2%$ at stage III, and $7.9{\pm}0.9%$ at stage IV. The flotation rate and hatching rate were highest at stage III, and the fertilization rate and embryonic survival rate were highest at stage IV. Therefore, in terms of egg quality, the amount of eggs collected per mother fish, maturation, and histology were different depending on the degree of abdomen inflation. At stage III, where the abdomen inflation degree of the mother fish was based on the basal part of the dorsal fin relative to the height of the anal fin was 1, the egg quality was highest.
We determined the morphologic characteristics (body weight and degree of abdomen inflation) of the red spotted grouper, Epinephelus akaara, mother fish producing healthy eggs. Experimental fish were chosen from fish reared in a sea cage. The fish were divided into four size groups by body weight: 400~600, 600~800, 800~1,000, and 1,000~1,200 g and four stages (I~IV) of the degree of abdomen inflation. After hormone treatment, we observed the amount of ovulation-induced eggs, and rates of buoyancy, fertilization, embryonic survival, and hatching. As a result, mother fish with a body weight of 600 g or more spawned, and the fertilization rate, embryonic survival rate, and hatching rate were high in the 800~1,000 g range, thus showing effective ovulation induction. As a result of dividing the degree of abdomen inflation based on the anal fin of the mother fish into I-IV stages and determining hormone treatment time, the GSI was $0.9{\pm}0.2%$ at stage I, $2.3{\pm}0.2%$ at stage II, $5.6{\pm0.2%$ at stage III, and $7.9{\pm}0.9%$ at stage IV. The flotation rate and hatching rate were highest at stage III, and the fertilization rate and embryonic survival rate were highest at stage IV. Therefore, in terms of egg quality, the amount of eggs collected per mother fish, maturation, and histology were different depending on the degree of abdomen inflation. At stage III, where the abdomen inflation degree of the mother fish was based on the basal part of the dorsal fin relative to the height of the anal fin was 1, the egg quality was highest.
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문제 정의
Therefore, this study was conducted to establish the hormone treatment time and screening criteria for target mother fish for seed production based on the external form of a red spotted grouper mother candidate group. This study also aimed to provide a framework for stable production of high-quality embryos by easily applying these criteria in mass seed production in the industry.
Therefore, this study was conducted to establish the hormone treatment time and screening criteria for target mother fish for seed production based on the external form of a red spotted grouper mother candidate group. This study also aimed to provide a framework for stable production of high-quality embryos by easily applying these criteria in mass seed production in the industry. The degree of abdomen inflation of the mother fish based on the anal fin was classified, gonadosomatic index (GSI) was determined, and ovaries and eggs were observed according to the degree of abdomen inflation (Miura et al.
제안 방법
To determine the minimum body weight required for egg maturation and ovulation induction, 40 female fish (32.5±1.7 to 39.9±2.0 cm in total length, 550.7±65.5 to 1,110.0±65.8 g in body weight) were divided into four weight groups (400–600, 600–800, 800–1,000, and 1,000–1,200 g).
Ten collected embryos were observed under a stereoscopic microscope (Olympus CX41, Japan) to measure egg diameter and oil globule diameter. The eggs and semen were artificially fertilized using the dry method, and the fertilization rate and floatation rate were examined. The embryonic survival rate until hatching and hatching rate 12 hours after hatching were determined.
Then, 10 fish were selected from each group and treated with the hormone. Next, the frequency of spawning, amount of eggs collected per mother fish, fertilization rate, floatation rate, embryonic survival rate, and hatching rate were examined following spawning after hormone treatment (Table 1).
The egg characteristics (egg diameter and oil globule diameter) and GSI were measured to investigate the sexual maturity stage according to the degree of abdomen inflation. As a result, at stage I, the egg diameter was 0.
The size of oocytes was measured through cannulation during the spawning season for seed production to determine hormone treatment time. In this study, we aimed to verify the applicability of determining hormone treatment time through the degree of abdomen inflation measured by the naked eye instead of determining hormone treatment time by measuring the size of oocytes through existing cannulation for seed production of E. septemfasciatus. As a result, the quality of eggs differed according to the degree of abdomen inflation of the mother fish in terms of the amount of eggs collected per mother fish, maturation degree, and histology.
대상 데이터
Three-hundred forty-three red spotted groupers (38.9±4.1 cm in total length, 885.3±143.5 g in weight) were caught in along the Geomun-do shore region in Yeosu, Jeollanam-do in June 2013 and cultured in a cage (5×5×5 m).
이론/모형
To histologically observe gonadal development at each stage, ovaries fixed in 10% neutral formalin were re-fixed with Bouin’s solution. The fixed tissues were prepared into blocks by the paraffin embedding method. Then, the samples were serially sectioned into 4 μm sections by the paraffin sectioning method.
성능/효과
, 2014). Fertilization rate, floatation rate, embryonic survival rate, and hatching rate based on the degree of abdomen inflation were examined, the possibility of screening mothers based on external forms was confirmed, and the quality of eggs was compared.
In conclusion, the results of this study suggest that hormone treatment time and mother screening criteria can be determined by visual methods, such as the degree of abdomen inflation, for seed production of red spotted grouper. If visual indicators that can be used as criteria for determining hormone treatment time and screening of mother fish are identified in addition to the degree of abdomen inflation, it would be possible to easily determine hormone treatment time and screen mother fish without causing stress to the mother fish, as it would not require cannulation.
참고문헌 (32)
Abdulfatah A, Fontaine P, Kestemont P, Milla S, Marie M (2013) Effects of the thermal threshold and the timing of temperature reduction on the initiation and course of oocyte development in cultured female of Eurasian perch Percafluvi atilis. Aquaculture 376-379:90-96.
Cho JK, Hong CG, Park JY, Son MY, Park JM, Han KH, Kim KM. 2016. Ovulation induction effect of Sevenband Grouper, Epinephelus septemfasciatus in size and abdomen inflation degree of mother fish. Korean J Ichthyol 28:260-266.
De Vlaming VL (1972) The effects of temperature and photoperiod on reproductive cycling in the estuarine gobiid fish, Gillichthys mirabilis. Fish Bull 70:1137-1152.
De Vlaming VL (1975) Effects of photoperiod and temperature on gonadal activity in the cyprinid teleost, Notemigonus crysoleucas. Biol Bull 148:402-415.
De Vlaming VL (1983) Oocyte development patterns and hormonal involvement among teleosts. In: Rankin JC, Pitcher TJ, Duggan R (ed), Control Processes in Fish Physiology. Croom Helm, London, pp 176-199.
Harikrishnan R, Kim JS, Balasundaram C, Heo MS (2012) Immunomodulatory effects of chitin and chitosan enriched diets in Epinephelus bruneus against Vibrio alginolyticus infection. Aquaculture 326-329:46-52.
Hwang SI, Lee YD, Song CB, Rho S (1998) Gonadal development and the effects of $17{\alpha}$ -methyltestosterone on sex inversion of the red spotted grouper, Epinephelus akaara. J Aquac 11:173-182.
Hernandez MD, Egea MA, Rueda FM, Martinez FJ, Garcia B (2003) Seasonal condition and body composition changes in sharpsnout seabream (Diplodus puntazzo) raised in captivity. Aquaculture 220:569-580.
Kang GY, Song CB, Lee JH (2003) Cloning of growth hormone complementary DNA from red spotted grouper (Epinephelus akaara) and its expression in E. coli. J Aquac 16:110-117.
Kim IS, Choi Y, Kim BJ (2001). Percoidei fishes of Korea. Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea, p 279.
Kohno H, Diani S, Supriatna A (1993) Morphological development of larval and juvenile grouper, Epinephelus fuscoguttatus. Jpn J Ichthyol 40:307-316.
Lee TW, Lee CK (1996) Age and growth of Epinephelus akaara in the South Western Sea of Korea. Korean J Ichthyol 8:16-22.
Lee CK, Hur SB (1997) Yolk resorption, onset of feeding and survival potential of larvae of red spotted grouper, Epinephelus akaara. J Aquac 10:473-483.
Lee CK, Hur SB, Park S, Kim BG (1997) Qualities of spawned eggs during the spawning period in red spotted grouper, Epinephelus akaara. J Aquac 10:463-472.
Lee CK, Hur SB, Ko TS, Park S (1998) Maturation, sex ratio and sex-reversal of red spotted grouper, Epinephelus akaara. J Aquac 11:573-580.
Lee TY, Hanyu I, Furukawa K (1984) Effects of photoperiod and temperature on the gonadal activity in small filefish, Rudarius ercodes. Korean J Fish Aquat Soc 17:523-528.
Lee WO, Baek JN, Lee JH, Kim KH, Kim CH, Yoon SW (2012). Sexual maturation and feeding habit of Korean mandarin fish, Siniperca scherzeri (Perciformes, Centropomidae) in the Seomjin river and Imjin river, Korea. Kor J Env Eco 26: 57-66.
Liao IC, Leano EM (2008) The Aquaculture of Grouper. Asian Fisheries Society, pp 49-60.
Micale V, Perdichizzi F, Basciano G (1996) Aspects of the reproductive biology of the sharpsnout seabream Diplodus puntazzo (Cetti, 1777). I. Gametogenesis and gonadal cycle in captivity during the third year of life. Aquaculture 140:281-291.
Miura C, Yoshihara Y, Shimizu-Yamaguchi S, Hayashi D, Hamada K, Takeda Y, Miura M, Miura T (2014) Controlled feeding alleviates the reduced growth associated with spawning in farmed yellowtail (Seriola quinqueradiata). Aquaculture 424-425:10-17.
Noh CH, Kim KS, Myoung JG, Cho JK, Yun NJ, Lim HG, Bang IC (2015) The hatchability of fertilized eggs of interspecific hybrid between red spotted grouper (Epinephelus akaara) and brown-marbled grouper (E. fuscoguttatus). Korean J Ichthyol 27:16-20.
Okumura S, Okamoto K, Oonori R, Nakazono A (2002) Spawning behavior and artificial fertilization in captive reared red spotted grouper, Epinephelus akaara. Aquaculture 206:165-173.
Papadaki M, Papadopoulou M, Siggelaki I, Mylonas CC (2008) Egg and sperm production and quality of sharpsnout sea bream (Diplodus puntazzo) in captivity. Aquaculture 276:187-197.
Park JY, Cho JK, Son MH, Kim KM, Han KH, Park JM (2016) Artificial spawning behavior and development of eggs, larvae and juveniles of the red spotted grouper, Epinephelus akaara in Korea. Dev Reprod 20:31-40.
Qiutao H, Gang L, Kai C, Enhui Z, Qiongshan F, Hansheng W, Jing L, Changjiang H, Qiaoxiang D (2011) Sperm cryopreservation of the endangered red spotted grouper, Epinephelus akaara, with a special emphasis on membrane lipids. Aquaculture 318:185-190.
Toledo JD, Nagi A, Javellana D (1993) Successive spawning of grouper, Epinephelus suillus (Valenciennes), in a tank and a floating net cage. Aquaculture 115:361-367.
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