[논문]난소적출 마우스에서 고진음자(固眞飮子) 물 추출물의 골다공증 개선 효과

조수연; 김동철

doi:10.15204/jkobgy.2018.31.4.016

난소적출 마우스에서 고진음자(固眞飮子) 물 추출물의 골다공증 개선 효과
Anti-osteoporotic Activity of Gojineumja Aqueous Extracts on the Ovariectomized Mice 원문보기

大韓韓方婦人科學會誌 = The Journal of Korean obstetrics & gynecology, v.31 no.4, 2018년, pp.16 - 38

조수연 (대구한의대학교 한의과대학 부인과학교실) , 김동철 (대구한의대학교 한의과대학 부인과학교실)

초록
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목 적: 본 연구에서는 고진음자 물 추출물의 골다공증 개선 효과를 난소적출 마우스를 이용하여 risedronate sodium(RES) 2.5 mg/kg 투여군과 비교 평가하였다. 방 법: OVX 35일 후부터 고진음자 물 추출물을 매일 1회씩 35일간 연속 경구 투여하고, 체중, 대퇴골의 중량, 골밀도, 골강도, 무기질 - 칼슘(Ca) 및 무기인(inorganic phosphorus, IP) 함량, 골량 및 구조와 골흡수에 관한 조직병리학적 변화를 혈중 osteocalcin 함량 및 bone specific alkaline phosphatase(BALP) 활성의 변화와 함께 각각 측정하였다. 본 실험에서 고진음자 물 추출물에서의 결과는 RES 경구 투여 OVX 마우스에서의 결과와 비교 평가 하였다. 결 과: OVX대조군에서는 현저한 체중 증가와 함께 대퇴골의 중량, 골밀도, 골강도, 무기질 - Ca 및 IP 함량 감소 및 지주골과 피질골의 현저한 조직병리학적 감소가 함께 확인되었으며, 혈중 osteocalcin 함량의 증가와 함께 혈중 BALP 활성의 감소가 각각 확인되었다. 이에, 전형적인 estrogen 결핍성 골다공증 소견이 OVX 수술에 의해 유발되는 것으로 관찰되었다. 한편 이렇게 OVX에 의해 유발된 estrogen 결핍성 골다공증 소견이 모든 세 용량의 고진음자 물 추출물 500, 250 및 125 mg/kg의 35일에 걸친 연속 경구 투여에 의해 투여 용량 의존적으로 현저히 억제되었고, 특히 고진음자 물 추출물 500 mg/kg은 RES 2.5 mg/kg 투여군과 비교할 만한 골다공증 개선 효과를 나타내었다. 결 론: 이상의 결과에서, 고진음자 물 추출물은 난소적출 마우스에서 투여 용량 의존적으로 유효한 골다공증 개선 효과를 나타내는 것으로 관찰되었다. 따라서 적절한 용량의 고진음자 물 추출물은 새로운 보다 효과적인 estrogen 결핍성 골다공증 개선제로서의 개발 가능성이 매우 높을 것으로 기대된다. 한편 고진음자는 15종의 약제로 구성되어 있으며, 각각의 개별 약제는 수많은 생리활성 물질을 함유하고 있어, 앞으로 생리활성을 나타내는 화학성분의 검색과 함께 다양한 방면으로 기전연구가 추가적으로 수행되어야 할 것으로 판단된다.

Abstract ▼ AI-Helper

Objectives: The objective of this in vivo study is to observe the anti-osteoporotic activities of Gojineumja aqueous extracts (GJEJ) on the ovariectomized (OVX) mice as compared to those of risedronate sodium (RES). Methods: Thirty five days after bilateral OVX, GJEJ was orally administered, for 35 days once a day and then the changes on the body weight and gain during experimental periods, femur weights, bone mineral density (BMD), bone strength (failure load), mineral contents - calcium (Ca) and inorganic phosphorus (IP), histological profiles and histomorphometrical analyses at sacrifice were conducted with serum biochemistry - osteocalcin contents and bone specific alkaline phosphatase (BALP) activities. And the results of GJEJ were compared with RES orally administered OVX mice. Results: As a result of OVX, noticeable increase of body weight and gains and serum osteocalcin levels, decrease of serum BALP activities, femur weights, femur Ca and IP contents, BMD and strength were observed as compared to those of sham control mice, respectively. Also, the decrease of all histomorphometrical indices indicating the bone mass and structure, and the increase of indices about resorption were also detected in the femur of OVX control. However, these estrogen-deficient osteoporotic signs were significantly and dose-dependently inhibited by 35 days of continuous oral treatment of GJEJ, at dose levels of 500, 250 and 125 mg/kg, respectively. Especially, GJEJ 500 mg/kg showed favorable inhibitory activities against estrogen-deficient osteoporosis symptoms induced by OVX as comparable to those of RES 2.5 mg/kg. Conclusions: The results in this study suggest that oral administrations of GJEJ have clear dose-dependent favorable anti-osteoporotic activities in OVX mice.

주제어

표/그림 (11)

표 Table 1. Composition of GJEJ Used in This Study
표 Table 2. Experimental Design Used in This Study
그림 Fig. 1. Body weights observed in Shamoperated and OVX mice. Values are expressed as Mean of eight mice. The day O -1 means 1 day before OVX. The day O means the day of OVX. The day -1 means 1 day before start of administration at 34 days after OVX. The day 0 means at start of administration, at 35 days after OVX. The day 35 means 35 days after start of administration, at sacrifice. All animals were overnight fasted before OVX, first administration and sacrifice, respectively.
표 Table 3. Right Femur Absolute Weights in Sham-operated and OVX Mice
표 Table 4. Right Femur Relative Weights in Sham-operated and OVX Mice
그림 Fig. 2. Changes on the serum osteocalcin levels in Sham-operated and OVX mice. a : p<0.01 as compared to sham control by LSD test b : p<0.01 as compared to OVX control by LSD test
그림 Fig. 3. Changes on the serum BALP levels in Sham-operated and OVX mice. a : p<0.01 as compared to sham control by LSD test b : p<0.01 as compared to OVX control by LSD test c : p<0.05 as compared to OVX control by LSD test
표 Table 5. Total Body and Right Femur BMD, Right Femur Strength in Shamoperated and OVX Mice
표 Table 6. Right Femur Mineral Contents in Sham-operated and OVX Mice
표 Table 7. Left Femur Histomorphometry in Sham-operated and OVX Mice: Trabecular Bones
표 Table 8. Left Femur Histomorphometry in Sham-operated and OVX Mice: Cortical Bones and Osteoclast Cells

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