[논문]New records of 24 ciliate species (Protozoa, Ciliophora) collected in South Korea

Kim, Ji Hye; Moon, Ji Hye; Jung, Jae-Ho

doi:10.12651/jsr.2018.7.4.291

New records of 24 ciliate species (Protozoa, Ciliophora) collected in South Korea 원문보기

Journal of species research, v.7 no.4, 2018년, pp.291 - 314

Kim, Ji Hye (Natural Science Research Institute, Gangneung-Wonju National University) , Moon, Ji Hye (Department of Biology, Gangneung-Wonju National University) , Jung, Jae-Ho (Department of Biology, Gangneung-Wonju National University)

Abstract ▼ AI-Helper

We collected ciliates from terrestrial and aquatic habitats in Korea. Morphology was examined based on observations of both living and stained specimens. As a result of the observations, we found 24 previously unrecorded ciliate species to Korea. The morphology of these species are briefly described here: Tracheloraphis dracontoides, Condylostoma arenarium, Aspidisca steini, Australocirrus aspoecki, Sterkiella tricirrata, Stylonychia lemnae, Wallackia bujoreani, Bakuella marina, Anteholosticha sigmoidea, Holosticha pullaster, Urostyla grandis grandis, Chaenea vorax, Enchelys gasterosteus, Phialina salinarum, Mesodinium pulex, Aegyria oliva, Bryometopus pseudochilodon, Pinacocoleps tesselatus, Urozona buetschlii, Colpidium colpoda, Campanella umbellaria, Pseudovorticella difficilis, Zoothamnium parahiketes and Z. plumula. Among these 24 ciliates, S. tricirrata and U. grandis grandis were studied based on both the morphology and the morphogenesis.

주제어

표/그림 (25)

그림 Fig. 1. Tracheloraphis dracontoides in vivo (A, B) and after protargol impregnation (C-E). A. Living individual to show extended body shape. B. Cortical granulation on glabrous stripe. C. Longitudinally arranged somatic kineties. D. Circumoral kinety (arrowhead) and brosse (arrows). E. Nuclear group. Scale bars=500 μm (A); 30 μm (B).
그림 Fig. 2. Condylostoma arenarium in vivo (A-C) and after protargol impregnation (D, E). A. Typical individual. B. Frontal cirri (arrowheads). C. Cortical granulation. D. Oral and somatic ciliature on anterior part (arrowheads indicate frontal cirri). E. Macronuclear nodules. Scale bars=200 μm (A); 10 μm (C).
그림 Fig. 3. Aspidisca steini protargol impregnated specimen (A, B). A. Ventral view of typical individual. B. Dorsal view. 1-4, dorsal kinety1-4. Scare bar=20 μm.
그림 Fig. 4. Australocirrus aspoecki in vivo (A, B) and after protargol impregnation (C). A. Living cell to show body shape. B. Reddish cortical granules and dorsal brush. C. Ventral view. Scale bars=100 μm (A); 50 μm (C); 10 μm (B).
표 Table 1. Morphometry of Sterkiella tricirrata.
그림 Fig. 5. Sterkiella tricirrata in vivo (A-C), protargol impregnated specimen (D-L) and morphogenetic stages (E-L). A. Typical individual to show ellipsoidal body shape (arrowhead indicates contractile vacuole). B. Transverse cirri (arrow). C. Dorsal bristles (arrowheads). D. Ventral view. E. Ventral view of early divider; asterisk indicates a very early divider to show oral primordium. F, H, J. Ventral views of middle dividers. G, I. Dorsal views of middle dividers. K. Ventral view of late divider. L. Dorsal view of late divider. Scale bars=20 μm.
그림 Fig. 6. Stylonychia lemnae in vivo (A) and after protargol impregnation (B, C). A. Typical individual. B. Oral and somatic ciliature on ventral view. C. Dorsal view. 1-6, dorsal kinety 1-6. Scale bars=100 μm (A); 50 μm (B, C).
그림 Fig. 7. Wallackia bujureani in vivo (A, B) and after protargol impregnation (C, D). A. Typical individual. B. Dorsal view. C. Ventral view. D. Dorsal view to show dorsal kineties and caudal cirri. Scale bars=50 μm (A); 30 μm (C).
그림 Fig. 8. Bakuella marina in vivo (A-C) and protargol impregnated specimen (D). A. Typical individual (arrow indicates contractile vacuole). B. Cortical granulation on ventral side. C. Cortical granulation on dorsal side. D. Oral and somatic ciliature on ventral view (arrows indicate frontoterminal cirri). Scale bars=50 μm.
그림 Fig. 9. Anteholosticha sigmoidea in vivo (A, B) and protargol impregnated specimen (C, D). A. Typical individual. B. Cortical granules on dorsal side. C. Ventral view to show macronuclear nodules. D. Somatic ciliature on ventral view. Scale bars=30 μm.
그림 Fig. 10. Holosticha pullaster in vivo (A-D) and protargol impregnated specimen (E). A. Typical individual and contractile vacuole (arrow). B. Contractile vacuole (arrow). C. Ventral view (arrowheads indicate cytoplasmic granules). D. Dorsal view (arrowheads indicate cytoplasmic granules). E. Oral and somatic ciliature. Scale bars=20 μm.
그림 Fig. 11. Urostyla grandis grandis after protargol impregnation. A. Vegetative stage. B. Middle divider (arrowheads indicate anlagen of left marginal rows). C. Late divider (arrowheads indicate anlagen of left marginal rows). Scale bars=20 μm.
그림 Fig. 12. Chaenea vorax in vivo (A, B) and after protargol impregnation (C-E). A. Typical individual. B. Oral and cytoplasmic extrusomes and contractile vacuole (arrows indicate food vacuoles). C. Somatic ciliature. D. Detail of dorsal brush rows (arrows). E. Macronuclear nodules (arrowheads). Scale bars=50 μm (A); 30 μm (C).
그림 Fig. 13. Enchelys gasterosteus in vivo (A-D) and after protargol impregnation (E). A. Typical individual. B. Conical structure after specimen squashed. C. Cytoplasmic inclusions with extrusomes and contractile vacuole. D. Cortical granules. E. Somatic ciliature with dorsal brush row (arrow) and micro-(triangular arrowhead) and macronucleus (arrowhead). Scale bars=50 μm (A); 10 μm (B, D).
그림 Fig. 14. Phialina salinarum in vivo (A-C) and after protargol impregnation (D). A. Typical individual. B. Details of oral extrusomes and macronucleus with cytoplasmic inclusions. C. Cortical granules. D. Oral and somatic ciliature with macronuclear apparatus. Scale bars=50 μm (A); 30 μm (D).
그림 Fig. 15. Mesodinium pulex in vivo (A) and after protargol impregnation (B, C). A. Typical individual. B. Two macronuclear nodules. C. Somatic ciliature. Scale bars=30 μm (A); 10 μm (B, C).
그림 Fig. 16. Aegyria oliva after protargol impregnation. A. Ventral view. B. Dorsal view showing ciliature, pharyngeal rods and macronuclear apparatus. Scale bar = 50 μm.
그림 Fig. 17. Bryometopus pseudochilodon in vivo (A, B), after protargol impregnation (C) and DAPI staining (D). A. Typical individual. B. Contractile vacuole (arrow). C. Oral ciliature. D. Ellipsoidal macronucleus and single spherical micronucleus (arrowhead). Scale bars=30 μm.
그림 Fig. 18. Pinacocoleps tesselatus in vivo (A-C), after protargol impregnation (D, E) and scanning electron microscope (F). A. Typical individual and caudal cilium (arrow). B. Contractile vacuole and posterior spine. C. Surface of armor plates. D. Somatic kineties and macronucleus. E. Adoral organelles (arrowheads). F. Posterior spines (arrows). Scale bars=50 μm (A), 30 μm (D).
그림 Fig. 19. Urozona buetschlii after protargol impregnation. A. Oral and somatic ciliature on ventral side. B. Equatorially restricted somatic ciliature on dorsal view and spherical macronucleus. Scale bars=10 μm.
그림 Fig. 20. Colpidium colpoda after protargol (A-D) and 'dry' silver nitrate impregnation (E). A. Ventral ciliature. B. Dorsal ciliature. C. Detail of oral ciliature. D. Macro- and micronucleus. E. Two secondary meridians between primary meridians in silverline system. Scale bar=30 μm (A).
그림 Fig. 21. Campanella umbellaria in vivo (A-C), after protargol (D, E) and 'dry' silver nitrate impregnation (F). A. Typical colony. B. Rigid stalk. C. Typical zooid. D. Infundibular polykinety. E. Haplo- and polykinety with macronucleus. F. Rectangular silverline system. Scale bars=1000 μm (A), 100 μm (C).
그림 Fig. 22. Pseudovorticella difficilis in vivo (A, B), after protargol (C, D) and 'wet' silver nitrate impregnation (E). A. Typical individuals and annular swelling (arrow) on stalk. B. Contractile vacuole (arrowhead) and annular swelling (arrow) on stalk. C. Infundibular polykinety 1-3 (arrowhead indicates longer and closely spaced outer two rows). D. Epistomial membrane (arrowhead) and macronucleus (arrow). E. Rectangular silverlines (arrow indicates trochal band). Scale bars=100 μm (A), 50 μm (B).
그림 Fig. 23. Zoothamnium parahiketes in vivo (A, B), after protargol (C, D) and 'wet' silver nitrate impregnation (E). A. Colony shape. B. Shape of zooids. C. Macro-(arrow) and micronucleus (arrowhead). D. Infundibular polykinety 2 (P2) (arrowhead) and P3 (arrow). E. Silverline system (arrow indicates trochal band). Scale bars=100 μm (A), 50 μm (B).
그림 Fig. 24. Zoothamnium plumula in vivo (A, B) and after protargol impregnation (C, D). A. Colony shape. B. Zooids and branching patterns. C. Infundibular polykinety 1 (P1) (arrow), P2 (triangular arrowhead) and P3 (arrowhead). D. Haplo- and polykinety with epistomial membrane 1 (EM1) (arrow) and EM2 (arrowhead) on apical view. Scale bars=1000 μm (A), 100 μm (B).

참고문헌 (46)

Berger, H. 1999. Monograph of the Oxytrichidae (Ciliophora, Hypotrichia). Monogr Biol 78:1-1080.
Berger, H. 2006. Monograph of the Urostyloidae (Ciliophora, Hypotrichia). Monogr Biol 85:1-1303.
Berger, H. 2011. Monograph of the Gonostomatidae and Kahliellidae (Ciliophora, Hypotricha). Monogr Biol 90:1-741.
Berger, H. and W. Foissner. 1989. Morphology and biometry of some soil hypotrichs (Protozoa, Ciliophora) from Europe and Japan. Bull Br Mus nat Hist (Zool) 55:19-46.
Borror, A.C. 1963. Morphology and ecology of the benthic ciliated protozoa of Alligator Harbor, Florida. Arch Protistenk 106:465-534.
Borror, A.C. 1972. Tidal marsh ciliates (Protozoa): morphology, ecology, systematics. Acta Protozool 31:29-71.
Chen, X., L. Li, S.A. Al-Farraj, H. Ma and H. Pan. 2018. Taxonomic studies on Aegyria apoliva sp. nov. and Trithigmostoma cucullulus (Muller, 1786) Jankowski, 1967 (Ciliophora, Cyrtophoria) with phylogenetic analyses. Eur J Protistol 62:122-134.

상세보기
Fan, X., Y. Xu, F. Gu, J. Li, S.A. Al-Farraj, K.A.S. Al-Rasheid and X. Hu. 2015. Morphology of two novel species of Chaenea (Ciliophora, Litostomatea): Chaenea paucistriata spec. nov. and C. sinica spec. nov. Acta Protozool 54:97-106.

상세보기
Fernandes, N.M., R.J.P. Dias, C.G. Schrago and I.D. Silva-Neto. 2015. Redescription and phylogenetic position of Condylostoma arenarium Spiegel, 1926 (Ciliophora, Heterotrichea) from Guanabara Bay, Brazil. J Eukaryot Microbiol 62:722-732.

상세보기
Foissner, W. 1982. Ecology and taxonomy of the Hypotrichida (Protozoa: Ciliophora) of some Austrian soils. Arch Protistenk 126:19-143.

상세보기
Foissner, W. 1984. Infraciliatur, Silberliniensystem und Biometrie einiger neuer und wenig bekannter terrestrischer, limnischer und mariner Ciliaten (Protozoa: Ciliophora) aus den Klassen kinetofragminophora, Colpodea und Polyhymenophora. Stapfia 12:1-165.
Foissner, W. 1993. Colpodea (Ciliophora). Protozoenfauna 4/1:1-798.
Foissner, W. 2004. Some new ciliates (Protozoa, Ciliophora) from an Austrian floodplain soil, including a giant, red "flagship", Cyrtohymena (Cyrtohymenides) aspoecki nov. subgen., nov. spec. Denisia 13:369-382.
Foissner, W. 2014. An update of 'basic light and scanning electron microscopic methods for taxonomic studies of ciliated protozoa'. Int J Syst Evol Microbiol 64:271-292.

상세보기
Foissner, W. and J. Dragesco. 1996. Updating the trachelocercids (Ciliophora, Karyorelictea). III. Redefinition of the genera Trachelocerca Ehrenberg and Tracheloraphis Dragesco, and evolution in trachelocercid ciliates. Arch Protistenkd 147:43-91.

상세보기
Foissner, W. and H. Schiffmann. 1974. Vergleichende Studien an argyrophilen Strukturen von vierzehn peritrichen Ciliaten. Protistologica 10:486-508.
Foissner, W., H. Berger and F. Kohmann. 1992. Taxonomische und Okologische Revision der Ciliaten des Saprobiensystems. Band II: Peritrichia, Heterotrichida, Odontostomatida. Informationsberichte des Bayer Landesamtes fur Wasserwirtschaft 5/92:394-399.
Foissner, W., H. Berger and F. Kohmann. 1994. Taxonomische und Okologische Revision der Ciliaten des Saprobiensystems. Band III: Hymenostomata, Prostomatida, Nassulida. Informationsberichte des Bayer Landesamtes fur Wasserwirtschaft 1/94:1-548.
Foissner, W., H. Berger and J. Schaumberg. 1999. Identification and ecology of limnetic plankton ciliates, Informationsberichte des Bayer Landesamtes fur Wasserwirtschaft 3/99:1-793.
Foissner, W., S. Agatha and H. Berger. 2002. Soil ciliates (Protozoa, Ciliophora) from Namibia (Southwest Africa), with emphasis on two contrasting environments, the Etosha region and the Namib desert. Part I: Text and line drawings. Denisia 5:1-1063.
Foissner, W., H. Blatterer, H. Berger and F. Kohmann. 1995. Taxonomische und Okologische Revision der Ciliaten des Saprobiensystems. Band IV: Gymnostomatea, Loxodes, Suctoria. Informationsberichte des Bayer Landesamtes fur Wasserwirtschaft 1/95:1-540.
Gong, J., A. Warren and W. Song. 2009. Chapter 2. Cyrtophorids. In W. Song, A. Warren, X. Hu (Eds.), Free-living ciliates in the Bohai and Yellow Seas. Science Press. Beijing, China pp. 49-92.
Ji, D., W. Song and J. Clamp. 2006. Pseudovorticella zhengae n. sp., P. difficilis (Kahl, 1933) Jankowski, 1976, and P. punctata (Dons, 1918) Warren, 1987, three marine peritrichous ciliates from north China. Eur J Protistol 42:269-279.

상세보기
Ji, D., P. Sun, A. Warren and W. Song. 2009. Chapter 8. Colonial sessilid peritrichs. In W. Song, A. Warren, X. Hu (Eds.), Free-living ciliates in the Bohai and Yellow Seas. Science Press. Beijing, China pp. 257-286.
Ji, D., J.H. Kim, S.U.A. Shazib, P. Sun, L. Li. and M.K. Shin. 2015. Two new species of Zoothamnium (Ciliophora, Peritrichia) from Korea, with new observations of Z. parahentscheli Sun et al., 2009. J Eukaryot Microbiol 62:505-518.

상세보기
Jung, J.-H., M.-H. Park, S.Y. Kim, J.M. Choi, G.-S. Min, and Y.-O. Kim. 2017. Checklist of Korean ciliates (Protozoa: Ciliophora). J Species Res 6:241-257.
Kahl, A. 1930. Urtiere oder Protozoa. I : Wimpertiere oder Ciliata (Infusoria) 1. Allgemeiner Teil und Prostomata. Tierwelt Deutschlands 18:1-180.
Kim, J.H. and J.-H. Jung. 2017. Cytological staining of protozoa: a case study on the impregnation of hypotrichs (Ciliophora: Spirotrichea) using laboratory-synthesized protargol. Anim Cells Syst 21:412-418.

상세보기
Kim, J.H. and J.-H. Jung. 2018. New record of five ciliates (Protozoa, Ciliophora) collected in eastern Gangwon-do Province, South Korea. J Species Res 7:181-186.
Kumar, S. and W. Foissner. 2015. Biogeographic specializations of two large hypotrich ciliates: Australocirrus shii and A. australis and proposed synonymy of Australocirrus and Cyrtohymenides. Eur J Protistol 51:210-228.

상세보기
Kumar, S., K. Kamra, D. Bharti and A. La Terza. 2015. Morphology, morphogenesis, and molecular phylogeny of Sterkiella tetracirrata n. sp. (Ciliophora, Oxytrichidae), from the Silent Valley National Park, India. Eur J Protistol 51: 86-97.

상세보기
Long, H., W. Song and A. Warren. 2009. Chapter 1. Haptorid and other lower kinetofragminophoran ciliates. In W. Song, A. Warren, X. Hu (Eds.), Free-living ciliates in the Bohai and Yellow Seas. Science Press. Beijing, China pp. 19-47.
Lu, B, J. Huang and X. Chen. 2013. The morphology and SSU rRNA gene sequence analysis of a poorly-known brackish water ciliate, Pinacocoleps tesselatus (Kahl, 1930) (Ciliophora, Colepidae) from Hangzhou Bay, China. Zootaxa 3637:123-130.

상세보기
Lynn, D.H. 2008. The ciliated protozoa: Characterization, classification, and guide to the literature. Springer, New York.
Morikawa, K. and M. Yanagida. 1981. Visualization of individual DNA molecules in solution by light microscopy: DAPI staining method. J Biochem 89:693-696.

상세보기
Olmo, J.L., C. Tellez and G.F. Esteban. 1998. Cinetozona pyriformis n. g., n. sp.: a relative of the ciliate Genera Urozona and Cinetochilum (Ciliophora, Scuticociliatida). J Euk Microbiol 45:448-451.

상세보기
Paiva, T.S., C. Shao, N.M. Fernandes, B.N. Borges and I.D. Silva-Neto. 2016. Description and phylogeny of Urostyla grandis wiackowskii subsp. nov. (Ciliophora, Hypotricha) from an estuarine mangrove in Brazil. J Eukaryot Microbiol 63:247-261.

상세보기
Park, M.-H., Y.-D. Han, C.B. Kwon, E.S. Lee, J.H. Kim, Y.S. Kang, S.-J. Kim, H.-M. Yang, T. Park, J.-S. Yoo, H.-J. Kil, E. Nam, M.K. Shin and G.-S. Min. 2017. Unrecorded species of Korean ciliates (Protozoa, Ciliophora) discovered through the project of "Discovery of Korean Indigenous Species" (2006-2010). J. Species Res., 6 (Special Edition): 172-176.
Shi, X., A. Warren, Y. Yu and Y. Shen. 2004. Infraciliature and myoneme system of Campanella umbellaria (Protozoa, Ciliophora, Peritrichida). J Morphol 261:43-51.

상세보기
Shin, M.K. 1994. Systematics of Korean hypotrichs (Ciliophora, Polyhymenophora, Hypotrichida) and molecular evolution of hypotrichs. Dissertation Seoul National University, Seoul pp. 1-270.
Song, W. and G. Packroff. 1996/97. Taxonomische Untersuchngen an marinen Ciliaten aus China mit Beschreibungen von zwei neuen Arten, Strombidium globosaneum nov. spec. und S. platum nov. spec. (Protozoa, Ciliophora) Arch Protistenkd 147:331-360.

상세보기
Song, W. and N. Wilbert. 1997. Morphological studies on some free living ciliates (Ciliophora: Heterotrichida, Hypotrichida) from marine biotopes in Qingdao, China, with descriptions of three new species: Holosticha warreni nov. spec., Tachysoma ovata nov. spec. and T. dragescoi nov. spec. Eur J Protistol 33:43-62.
Song, W., K.A.S. Al-Rasheid and X. Hu. 2002. Notes on the poorly-known marine peritrichous ciliate, Zoothamnium plumula Kahl, 1933 (Protozoa: Ciliophora), an ectocommensal organism from cultured scallops in Qingdao, China. Acta Protozool 41:163-168.

상세보기
Sun, P., D. Ji, A. Warren and W. Song. 2009. Chapter 7. Solitary sessilid peritrichs. In W. Song, A. Warren, X. Hu (Eds.), Free-living ciliates in the Bohai and Yellow Seas. Science Press. Beijing, China pp. 217-256.
Sun, P., W. Song, D. Ji and X. Hu. 2005. Redescription of Pseudovorticella cylindrica (Dons, 1915) nov. comb. and Zoothamnium hiketes Precht, 1935 two poorly defined marine peritrichs (Ciliophora: Peritrichia) from the north China Sea. J Nat Hist 39:2953-2965.

상세보기
Wu, I. and C. Curds. 1979. A guide to the species of Aspidisca. Bull Br Mus nat Hist (Zool), 36:1-34.

저자의 다른 논문 :

LOADING...

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증