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백편두의 항산화 물질 분리 및 동정
Purification and Identification of Antioxidant Compounds from Dolichos lablab L. Seeds 원문보기

韓國藥用作物學會誌 = Korean journal of medicinal crop science, v.27 no.6, 2019년, pp.419 - 426  

권남우 (충북대학교 특용식물학과) ,  김재연 (충북대학교 특용식물학과) ,  조용범 (충북대학교 특용식물학과) ,  황방연 (충북대학교 약학과) ,  김준구 (충북대학교 약학과) ,  우선희 (충북대학교 식물자원학과) ,  이문순 (충북대학교 특용식물학과)

Abstract ▼ AI-Helper 아이콘AI-Helper

Background: This study aimed to identify antioxidant compounds from the seeds of Dolichos lablab L. by bioassay-guided isolation and recrystallization. Methods and Results: The water layer of D. lablab L. seed extract inhibits intracellular reactive oxygen species (ROS) expressing the 2',7'-dichloro...

주제어

#Dolichos lablab L   #   #Chemical Structures   #Reactive Oxygen Species Inhibitory Effect   #Seed Extract   # $C_{14}H_{14}O_4N_2$   #Stachyose  

AI 본문요약
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제안 방법

  • All the experiments were reiterated at three independent replicates, and the results were presented as means ± standard deviation.
  • In this study, we analyzed antioxidant activity by lipopolysaccharide (LPS)-stimulated RAW264.7 cells in D. lablab extract and segregated substances that exhibit antioxidant activity via open column, high-performance liquid chromatography (HPLC). And the structure of substances was identified by electrospray ionization-mass spectrometry (ESI-MS) and nuclear magnetic resonance (NMR).

대상 데이터

  • The RAW264.7 cells were purchased from Korea Cell Line Bank (KCLB, Seoul, Korea). They were cultured in Dulbecco’s modified eagle’s medium (DMEM, GibcoTM, Thermo Fisher Scientific, Waltham, MA, USA) and supplemented with 10% fetal bovine serum (FBS, Seradigm, Radnor, PA, USA), 1% penicillin-streptomycin (Sigma-Aldrich Co.

데이터처리

  • Statistical significance was determined by Duncan’s Multiple Range Tests (DMRT) using SPSS 12.0 windows version (SPSS Inc., Chicago, IL, USA).
본문요약 정보가 도움이 되었나요?

참고문헌 (23)

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    상세보기 crossref

  2. Bedard K and Krause KH. (2007). The NOX family of ROSgenerating NADPH oxidases: Physiology and pathophysiology. Physiological Reviews. 87:245-313. 

    상세보기 crossref

  3. Brzozowski T, Konturek PC, Konturek SJ, Kwiecien S, Sliwowski Z, Pajdo R, Duda A, Ptak A and Hahn EG. (2003). Implications of reactive oxygen species and cytokines in gastroprotection against stress-induced gastric damage by nitric oxide releasing aspirin. International Journal of Colorectal Disease. 18:320-329. 

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  4. Cerecetto H, Gerpe A, Gonzalez M, Aran VJ and de Ocariz CO. (2005). Pharmacological properties of indazole derivatives: Recent developments. Mini-Reviews in Medicinal Chemistry. 5:869-878. 

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  5. Gaikwad DD, Chapolikar AD, Devkate CG, Warad KD, Tayade AP, Pawar RP and Domb AJ. (2015). Synthesis of indazole motifs and their medicinal importance: An overview. European Journal of Medicinal Chemistry. 90:707-731. 

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  6. Guzik TJ, Korbut R and Adamek-Guzik T. (2003). Nitric oxide and superoxide in inflammation. Journal of Physiology and Pharmacology. 54:469-487. 

  7. Huang G, Mao J, Ji Z and Ailati A. (2015). Stachyose-induced apoptosis of caco-2 cells via the caspase-dependent mitochondrial pathway. Food and Function. 6.3:765-771. 

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  8. Im AR, Kim YH, Lee HW and Song KH. (2016). Water extract of Dolichos lablab attenuates hepatic lipid accumulation in a cellular nonalcoholic fatty liver disease model. Journal of Medicinal Food. 19:495-503. 

    상세보기 crossref

  9. Kante K and Reddy CS. (2013). Anti diabetic activity of Dolichos lablab(seeds) in streptozotocin-nicotinamide induced diabetic rats. Hygeia Journal for Drugs and Medicines. 5:32-40. 

  10. Michiels C, Raes M, Toussaint O and Remacle J. (1994). Importance of Se-glutathione peroxidase, catalase, and Cu/Zn-SOD for cell survival against oxidative stress. Free radical Biology and Medicine. 17:235-248. 

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  11. Nishizawa-Yokoi A, Yabuta Y and Shigeoka S. (2008). The contribution of carbohydrates including raffinose family oligosaccharides and sugar alcohol to protection of plant cells from oxidative damage. Plant Signaling and Behavior. 3:1016-1018. 

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  12. Niu XF, Smith CW and Kubes P. (1994). Intracellular oxidative stress induced by nitric oxide synthesis inhibition increases endothelial cell adhesion to neutrophils. Circulation Research. 74:1133-1140. 

    상세보기 crossref

  13. Osman MA. (2007). Effect of different processing methods, on nutrient composition, antinutrional factors, and in vitro protein digestibility of Dolichos lablab bean[Lablab purpuresus (L) Sweet]. Pakistan Journal of Nutrition. 6:299-303. 

    상세보기 crossref

  14. Peterbauer T and Richter A. (2001). Biochemistry and physiology of raffinose family oligosaccharides and galactosyl cyclitols in seeds. Seed Science Research. 11:185-197. 

    상세보기

  15. Priya S and Jenifer S. (2014). Antibacterial activity of leaf and flower extract of Lablab purpureus against clinical isolates of Staphylococcus aureus. Research and Reviews: A Journal of Drug Design and Discovery. 1:5-7. 

  16. Rahman A, Malik S, Hasan SS, Choudhary MI, Ni CZ and Clardy J. (1995). Nigellidine: A new indazole alkaloid from the seeds of Nigella sativa. Tetrahedron Letters. 36:1993-1996. 

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  17. Ramakrishna V, Jhansi Rani P and Pao RR. (2007). Hypocholesterolemic effect of diet supplemented with Indian bean (Dolichos lablab L. var lignosus) seeds. Nutrition and Food Science. 37:452-456. 

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  18. Salvemini D, Ischiropoulos H and Cuzzocrea S. (2003). Roles of nitric oxide and superoxide in inflammation. In Winyard PG and Willoughby DA (ed.). In Inflammation Protocols. Methods in Molecular Biology. vol 225. Humana Press. Totowa, NJ, USA. p.291-303. 

  19. Stoyanova S, Geuns J, Hideg E and Van Den Ende W. (2011). The food additives inulin and stevioside counteract oxidative stress. International Journal of Food Sciences and Nutrition. 62:207-214. 

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  20. Takeuchi A, Hori M, Sato S, Ban HS, Kuchimaru T, Kizaka-Kondoh S, Yamori T and Nakamura H. (2012). Synthesis and biological activity of furanylindazoles as inhibitors of hypoxia inducible factor(HIF)-1 transcriptional activity. Medicinal Chemistry. Communication. 3:1455-1461. 

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  21. Tiidus PM. (1998). Radical species in inflammation and overtraining. Canadian Journal of Physiology and Pharmacology. 76:533-538. 

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  22. Warleta F, Quesada CS, Campos M, Allouche Y, Beltran G and Gaforio JJ. (2011). Hydroxytyrosol protects against oxidative DNA damage in human breast cells. Nutrients. 3:839-857. 

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  23. Ye XY, Wang HX and Ng TB. (2000). Dolichin, a new chitinaselike antifungal protein isolated from field beans(Dolichos lablab). Biochemical and Biophysical Research Communications. 269:155-159. 

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