[논문]섬개야광나무의 부위별 메탄올 추출물의 항산화 및 항염 활성 비교

유남호; 김희규; 이찬옥; 박주희; 김명조

doi:10.7783/kjmcs.2019.27.3.194

섬개야광나무의 부위별 메탄올 추출물의 항산화 및 항염 활성 비교
Comparison of Anti-oxidant and Anti-inflammatory Activities of Methanolic Extracts Obtained from Different Parts of Cotoneaster wilsonii Nakai 원문보기

韓國藥用作物學會誌 = Korean journal of medicinal crop science, v.27 no.3, 2019년, pp.194 - 201

유남호 (강원대학교 생물자원과학부) , 김희규 (강원도산림과학연구원) , 이찬옥 (강원도산림과학연구원) , 박주희 ((주)램바이오 연구소) , 김명조 (강원대학교 생물자원과학부)

Abstract ▼ AI-Helper

Background: The Ministry of Environment, Korea recognizes Cotoneaster wilsonii Nakai as a second-class endangered plant. It is a native species that grows in Ulleung-do, Korea. To our knowledge, the bioactivity of this plant has not yet been reported. Therefore, in this study, we have reported the bioactivity of C. wilsonii Nakai. Methods and Results: The anti-oxidant activities of C. wilsonii methanolic extract were investigated in vitro. The anti-oxidant activity was evaluated using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical assay, and the total phenolic and flavonoid content were measured. The leaf methanolic extract had the highest DPPH radical scavenging activity ($IC_{50}$; $15.74{\mu}g/m{\ell}$), and it also had the highest total phenolic and flavonoid content ($220.95mg{\cdot}GAE/g$, and $36.46mg{\cdot}QE/g$ extract respectively). Through high performance liquid chromatography (HPLC) analysis, chlorogenic acid was found to be the predominant among all phenolic compounds, showing a concentration of 84.24 mg/g extract. More than 60% decrease in nitric oxide (NO) production was found in the leaf methanolic extract. Conclusions: To the best of our knowldege, this is the first report of the bioactivities of C. wilsonii. The results demonstrate that the leaf methanolic extract of C. wilsonii shows potent anti-oxidant, and anti-inflammatory activities.

주제어

표/그림 (8)

표 Table 1. DPPH radical scavenging activity of extractions from C. wilsonii Nakai.
그림 Fig. 1. Reducing power of extractions from C. wilsonii Nakai.
표 Table 2. The total phenolic and flavonoid contents of extractions from C. wilsonii Nakai.
그림 Fig. 2. Inhibitory effect of extract from C. wilsonii Nakai on cytotoxicity of RAW264.7 cell.
그림 Fig. 3. The effect of C. wilsonii Nakai on LPS-induced NO production in RAW264.7 cell.
그림 Fig. 4. HPLC chromatograms of standard compounds and methanolic extracts from C. wilsonii Nakai.
표 Table 3. Calibration curve of standard and limit of detection (LOD) and, limit of quantitation (LOQ).
표 Table 4. The difference in the content of phenolic acid composition of the C. wilsonii Nakai.

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문제 정의

The main aims of this study was to provide scientific basis for the possibility of development as a natural product material by evaluating the effects of anti-oxidant, anti-inflammatory activities.

제안 방법

Methanolic extraction was provided by Gangwon Nature Environment Research park (Hongcheon, Korea) and used for this experiment.

대상 데이터

Dulbecco’s modified eagle’s medium (DMEM), fetal bovine serum (FBS) and penicillin were purchased from biowest (Maine-et-Loire, France).
Macrophages (RAW264.7) used in the experiment was obtained from Korean Cell Line Bank (Seoul, Korea). Phosphate buffered saline (PBS), 1-(4,5-dimethylthiazol-2-yl)-3,5-diphenyl-formazan (MTT), lipopolysaccharides (LPS) used Sigma-Aldrich (St.
The HPLC analysis was analyzed using e2695 system of the Waters (Milford, MA, USA). The detector was 2489 UV-Vis detector of the Waters (Milford, MA, USA). The analytical column was a CAPCELL PAK C18 (5 ㎛, 4.

데이터처리

Overall differences among the treatment groups were determined using One-way analysis of variance (ANOVA) by IBM SPSS Statistic 24 Software (Armonk, NY, USA).
^*Each Data is means ± standard deviation of three replicate tests. The all data were statistically analyzed used by One-way analysis of variance (ANOVA). And differences were assessed that significant at 5% level of probability (p < 0.

이론/모형

DPPH radical scavenging activity was evaluated using the modifying method of Blois (1958).
, Tokyo, Japan). The phenolic content was calculated using the linear equation based on the gallic acid calibration curve. And the results were expressed as the gallic acid equivalence per gram of extract (㎎·GAE/g).

성능/효과

Researchers suspect that the seeds of Cotoneaster multiflorus floating in the sea or carried by birds or winds may have adapted to the unusual environmental conditions of Ulleung-do and have been differentiated into C. wilsonii Nakai. The height of C.
The results of the DPPH radical scavenging activity, the IC50 values of the stem methanolic extract was 22.00 ± 0.14 ㎍/㎖, the leaf methanolic extract was 15.74 ± 0.16 ㎍/㎖ and the fruit methanolic extract was 80.58 ± 0.84 ㎍/㎖ (Table 1).
Therefore, it was confirmed that the phenol content (214.65 ㎎·GAE/g) is higher in the stem methanolic extract of the C. wilsonii Nakai.

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