Zheng, Jianning
(Department of Chemical Engineering, Chungbuk National University)
,
Negi, Abhishek
(Department of Chemical Engineering, Chungbuk National University)
,
Khomlaem, Chanin
(Department of Chemical Engineering, Chungbuk National University)
,
Kim, Beom Soo
(Department of Chemical Engineering, Chungbuk National University)
Bioethanol has attracted much attention in recent decades as a sustainable and environmentally friendly alternative energy source. In this study, we compared the production of bioethanol by Candida molischiana and Saccharomyces cerevisiae at different initial concentrations of cellobiose and glucose...
Bioethanol has attracted much attention in recent decades as a sustainable and environmentally friendly alternative energy source. In this study, we compared the production of bioethanol by Candida molischiana and Saccharomyces cerevisiae at different initial concentrations of cellobiose and glucose. The results showed that C. molischiana can utilize both glucose and cellobiose, whereas S. cerevisiae can only utilize glucose. The ethanol yields were 43-51% from different initial concentrations of carbon source. In addition, different concentrations of microcrystalline cellulose (Avicel) were directly converted to ethanol by a combination of Trichoderma reesei and two yeasts. Cellulose was first hydrolyzed by a fully enzymatic saccharification process using T. reesei cellulases, and the reducing sugars and glucose produced during the process were further used as carbon source for bioethanol production by C. molischiana or S. cerevisiae. Sequential culture of T. reesei and two yeasts revealed that C. molischiana was more efficient for bioconversion of sugars to ethanol than S. cerevisiae. When 20 g/l Avicel was used as a carbon source, the maximum reducing sugar, glucose, and ethanol yields were 42%, 26%, and 20%, respectively. The maximum concentrations of reducing sugar, glucose, and ethanol were 10.9, 8.57, and 5.95 g/l, respectively, at 120 h by the combination of T. reesei and C. molischiana from 50 g/l Avicel.
Bioethanol has attracted much attention in recent decades as a sustainable and environmentally friendly alternative energy source. In this study, we compared the production of bioethanol by Candida molischiana and Saccharomyces cerevisiae at different initial concentrations of cellobiose and glucose. The results showed that C. molischiana can utilize both glucose and cellobiose, whereas S. cerevisiae can only utilize glucose. The ethanol yields were 43-51% from different initial concentrations of carbon source. In addition, different concentrations of microcrystalline cellulose (Avicel) were directly converted to ethanol by a combination of Trichoderma reesei and two yeasts. Cellulose was first hydrolyzed by a fully enzymatic saccharification process using T. reesei cellulases, and the reducing sugars and glucose produced during the process were further used as carbon source for bioethanol production by C. molischiana or S. cerevisiae. Sequential culture of T. reesei and two yeasts revealed that C. molischiana was more efficient for bioconversion of sugars to ethanol than S. cerevisiae. When 20 g/l Avicel was used as a carbon source, the maximum reducing sugar, glucose, and ethanol yields were 42%, 26%, and 20%, respectively. The maximum concentrations of reducing sugar, glucose, and ethanol were 10.9, 8.57, and 5.95 g/l, respectively, at 120 h by the combination of T. reesei and C. molischiana from 50 g/l Avicel.
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제안 방법
In order to solve the energy crisis and promote its use, it is essential to reduce the production cost by using cheap substrate along with increasing production efficiency using suitable microorganisms. In this study, we investigated the growth of C. molischiana and S. cerevisiae at different initial concentrations of glucose and cellobiose (20, 50, and 100 g/l) as carbon sources (Fig. 1). Growth of the yeast was quantified by optical density measurement (OD at 600 nm).
대상 데이터
Trichoderma reesei RUT-C30 (KCTC 6968) and Saccharomyces cerevisiae (KCTC 7928) were procured from Korean Collection for Type Cultures (Daejeon, Korea). Candida molischiana (ATCC 2516) was procured from American Type Culture Collection (USA).
Three fungal strains were used in this study. Trichoderma reesei RUT-C30 (KCTC 6968) and Saccharomyces cerevisiae (KCTC 7928) were procured from Korean Collection for Type Cultures (Daejeon, Korea).
Three fungal strains were used in this study. Trichoderma reesei RUT-C30 (KCTC 6968) and Saccharomyces cerevisiae (KCTC 7928) were procured from Korean Collection for Type Cultures (Daejeon, Korea). Candida molischiana (ATCC 2516) was procured from American Type Culture Collection (USA).
성능/효과
In conclusion, this study demonstrated that the use of C. molischiana was superior to S. cerevisiae for bioethanol production from cellobiose or Avicel as a carbon source. Ethanol yield from glucose and cellobiose was 43-51% in aerobic environment.
The highest ethanol yield from 20 g/l Avicel was 20% for the combination of T. ressei and C. molischiana and 13% for the combination of T. ressei and S. cerevisiae. The ethanol yield obtained with the combination of T.
cerevisiae. Therefore, it can be concluded that C. molischiana is more efficient for the production of bioethanol from cellulose than S. cerevisiae.
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