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[국내논문] New Downy Mildew Disease Caused by Hyaloperonospora brassicae on Pak choi (Brassica rapa) in Korea 원문보기

Research in plant disease = 식물병연구, v.25 no.2, 2019년, pp.98 - 101  

Lee, Hyun Ju (Gyeonggido Agricultural Research & Extension Services) ,  Lee, Jae Sung (Department of Biology, Kunsan National University) ,  Choi, Young-Joon (Department of Biology, Kunsan National University)

Abstract ▼ AI-Helper 아이콘AI-Helper

Pak choi (or a Bok choy; Brassica rapa subsp. chinensis) is a popular brassicaceous vegetable worldwide. In January 2019, a downy mildew symptom on pak choi was found at a farm located in Yongin, Korea. Based on morphological characteristics and molecular phylogenetic inference, the pathogenic oomyc...

주제어

#Brassica   #downy mildew   #Oomycota   #pak choi   #phylogeny  

표/그림 (2)

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제안 방법

  • For morphological identification, conidiophores and conidia protruding from the stomata of the infected leaves were placed on a drop of distilled water on a slide glass and covered with a cover slip. The preparate was examined under a DIC-light microscope BX53F (Olympus, Tokyo, Japan) and photographed with a DigiRetina 16M digital camera (Tucsen, Fuzhou, China). Initial downy mildew symptoms on the pak choi appeared as yellowish to green-yellowish spots on the upper surface of leaves (Fig.
  • The amplicons were purified using an AccuPrep PCR Purification Kit (Bioneer, Daejeon, Korea) and sequenced by a DNA sequencing service (Macrogen, Seoul, Korea), with the same primers used for amplification. The resulting sequences were edited with the DNASTAR software package version 5.05 (DNASTAR, Madison, WI, USA) and deposited in GenBank (accession nos. MN065156 for ITS rDNA, MN072364 for cox2 mtDNA). In addition to the reference sequences of Hyaloperonospora species available in NCBI GenBank, they were aligned by MAFFT 7 (Katoh and Standley, 2013).

대상 데이터

  • 1A). A representative herbarium specimen was preserved in the Kunsan National University Herbarium (KSNUH378). To identify the causal pathogen, morphological and molecular phylogenetic analyses were performed.
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참고문헌 (25)

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  2. Choi, Y. J., Shin, H. D. and Voglmayr, H. 2011. Reclassification of two Peronospora species parasitic on Draba in Hyaloperonospora based on morphological molecular and phylogenetic data. Mycopathologia 171: 151-159. 

  3. Choi, Y. J., Kim, J. Y., Park, J. H. and Shin, H. D. 2012. First report of tatsoi downy mildew caused by Hyaloperonospora brassicae in Korea. Plant Dis. 96: 1703. 

    상세보기

  4. Choi, Y. J., Beakes, G., Glockling, S., Kruse, J., Nam, B., Nigrelli, L. et al. 2015. Towards a universal barcode of oomycetes - a comparison of the cox1 and cox2 loci. Mol. Ecol. Resour. 15: 1275-1288. 

    상세보기 crossref

  5. Choi, Y.-J., Kruse, J. and Thines, M. 2018. Hyaloperonospora erucae sp. nov. (Peronosporaceae; Oomycota), the downy mildew pathogen of arugula (Eruca sativa). Eur. J. Plant Pathol. 151: 549-555. 

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  8. Fahey, J. 2003. Brassicas. In: Encyclopedia of food sciences and nutrition, eds. by B. Caballero, L. C. Trugo and P. M. Finglas, pp. 606-615. Academic Press, London, UK. 

  9. Farr, D. F. and Rossman, A. Y. 2019. Fungal databases, systematic mycology and microbiology laboratory. URL https://nt.ars-grin.gov/fungaldatabases/ [11 June 2019]. 

  10. Gaumann, E. A. 1923. Contributions toward a monograph of the genus Peronospora corda. Beitrage zur Kryptogamenflora der Schweiz 5: 1-360 (in German). 

  11. Goker, M., Riethmuller, A., Voglmayr, H., Weiss, M. and Oberwinkler, F. 2004. Phylogeny of Hyaloperonospora based on nuclear ribosomal internal transcribed spacer sequences. Mycol. Prog. 3: 83-94. 

  12. Goker, M., Voglmayr, H., Blazquez, G. G. and Oberwinkler, F. 2009. Species delimitation in downy mildews: the case of Hyaloperonospora in the light of nuclear ribosomal ITS and LSU sequences. Mycol. Res. 113: 308-325. 

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  13. Hong, S. Y., Jee, H. J., Choi, Y. J. and Shin, H. D. 2008. First confirmed report of downy mildew caused by Hyaloperonospora parasitica on broccoli in Korea. Plant Pathol. 57: 777. 

    상세보기

  14. Katoh, K. and Standley, D. M. 2013. MAFFT Multiple sequence alignment software version 7: Improvements in performance and usability. Mol. Biol. Evol. 30: 772-780. 

    상세보기 crossref

  15. Kluczewski, S. M. and Lucas, J. A. 1983. Host infection and oospore formation by Peronospora parasitica in agricultural and horticultural Brassica species. Trans. Br. Mycol. Soc. 81: 591-596. 

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  16. Kumar, S., Stecher, G. and Tamura, K. 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol. Biol. Evol. 33: 1870-1874. 

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  17. Lee, J. S., Lee, H. B., Shin, H.-D. and Choi, Y.-J. 2017. Diversity, phylogeny, and host-specialization of Hyaloperonospora species in Korea. Mycobiology 45: 139-149. 

    원문보기 상세보기 crossref

  18. Monot, C., Penguilly, D. and Silue, D. 2010. First confirmed report of downy mildew caused by Hyaloperonospora parasitica on broccoli, cauliflower and Romanesco-type cauliflower heads in France. Plant Pathol. 59: 1165. 

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  19. Sherriff, C. and Lucas, J. A. 1990. The host range of isolates of downy mildew, Peronospora parasitica, from Brassica crop species. Plant Pathol. 39: 77-91. 

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  20. Shin, H. D. and Choi, Y. J. 2006. Peronosporaceae of Korea. National Institute of Agricultural Science and Technology, Suwon, Korea. 166 pp. 

  21. The Korean Society of Plant Pathology. 2004. List of plant diseases in Korea. 4th ed. The Korean Society of Plant Pathology, Suwon, Korea. 779 pp. 

  22. Thines, M. and Choi, Y. J. 2016. Evolution, diversity, and taxonomy of the Peronosporaceae, with focus on the genus Peronospora. Phytopathology 106: 6-18. 

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  23. Voglmayr, H. and Goker, M. 2011. Morphology and phylogeny of Hyaloperonospora erophilae and H. praecox sp. nov., two downy mildew species co-occurring on Draba verna sensu lato. Mycol. Prog. 10: 283-292. 

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  24. Voglmayr, H., Choi, Y.-J. and Shin, H.-D. 2014. Multigene phylogeny, taxonomy and reclassification of Hyaloperonospora on Cardamine. Mycol. Prog. 13: 131-144. 

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  25. Yerkes, W. D. and Shaw, C. G. 1959. Taxonomy of the Peronospora species on Cruciferae and Chenopodiaceae. Phytopathology 49: 499-507. 

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