[논문]지실 추출물과 Naringin의 광방어 효과

박상희; 곽인실

doi:10.15207/jkcs.2019.10.7.267

지실 추출물과 Naringin의 광방어 효과
Effect of Photoprotective activities of Poncirustrifoliata immature Fruit extract and Naringin compound 원문보기

한국융합학회논문지 = Journal of the Korea Convergence Society, v.10 no.7, 2019년, pp.267 - 279

박상희 (대전과학기술대학교 피부보건과) , 곽인실 (호원대학교 미용예술학과)

초록
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본 연구는 지실추출물과 나린진의 햇빛으로부터 피부 보호 및 주름개선에 대한 연구를 하였다. HR-1(무모쥐)에 햇빛을 조사하여 상피 두께의 변화와 진피의 90% 정도를 차지하는 콜라겐 섬유의 변화를 확인하였다, 콜라게나아제의 억제 효능도 주름에 중요하게 작용하므로 같이 확인하였다. 실험은 나린진과 지실추출물을 사용하여 효능을 검증하였다. 첫째: 지실과 나린진의 성분을 분석하였다. 둘째: DPPH로 항산화 능력을 확인하였다. 셋째: 콜라겐의 변화를 측정하였다. 연구 결과로 지실추출물 및 나린진이 피부 상피두께 억제와 피부의 콜라겐 두께가 상승하였고 콜라겐 분해 효소 억제 및 주름이 대조군에 비하여 감소되었으며 유의한 차이가 나타났다.

Abstract ▼ AI-Helper

The study studied dermal protection and crease improvement from the sunlight of the lipid extract and narinjin. Sunlight was investigated in HR-1 (motherless mice) to identify changes in epithelial thickness and changes in collagen fibers, which account for around 90% of dermis, as the inhibitory efficacy of collagenase dissolving collagen also plays an important role in wrinkles. The experiment was validated using narinjin and jisil extract. First: The components of jisil and narinjin were analyzed. Second, antioxidant capabilities were confirmed with DPPH. Third: The inhibitory activity of collagen was measured. Studies have shown that the skin's upper skin thickness suppression of dermal extracts and narinzine has increased and that collagen thicknesses and wrinkles have decreased significantly compared to controls.

주제어

표/그림 (29)

표 Table 1. UVB irradiation and medication schedule
표 Table 2. Poncirus trifoliata Naringin in immature fruit and ointment cream formulations.
표 Table 3. Primer sequences (wrinkle-related genes) for real-time PCR analysis.
표 Table 4. Primer sequences (wrinkle-related genes) for real-time PCR analysis.
표 Table 5. Taqman probes and primer sequences (pro-inflammatory gene) for real-time PCR analysis.
그림 Fig. 1. Inhibition of skin thickening by PT_iF and naringin on epidermis induced by UVB irradiation.
그림 Fig. 2. Inhibition of skin thickening by vehicle control on epidermis induced by UVB irradiation.
그림 Fig. 3. HR-1 hairless male mice dorsal skin
그림 Fig. 4. Inhibition of skin thickening by 2.0% PT_iF on epidermis induced by UVB irradiation.
그림 Fig. 5. Inhibition of skin thickening by 0.25% naringin on epidermis induced by UVB irradiation.
그림 Fig. 6. Inhibition of skin thickening by 1.0% naringin on epidermis induced by UVB irradiation.
그림 Fig. 7. HR-1 hairless male mice dorsal skin
그림 Fig. 8. Collagen deposition by vehicle control on epidermis induced by UVB irradiation.
그림 Fig. 9. Collagen deposition by 0.5% PT_iF extract cream-ointment on epidermis induced by UVB irradiation.
그림 Fig. 10. Collagen deposition by 2% PT_iF extract cream-ointment on epidermis induced by UVB irradiation.
그림 Fig. 11. Collagen deposition by 0.25% naringin cream-ointment on epidermis induced by UVB irradiation.
그림 Fig. 12. Collagen deposition by 1.0% naringin cream-ointment on epidermis induced by UVB irradiation.
그림 Fig. 13. HR-1 hairless male mice dorsal skin on wrinkle formation
그림 Fig. 14. Effects of vihicle control on UVB-induced wrinkle formation in hairless mice
그림 Fig. 15. Effects of 2% PT_iF extract cream-ointment on UVB-induced wrinkle formation in hairless mice
그림 Fig. 16. Effects of 0.5% PT_iF extract cream-ointment on UVB-induced wrinkle formation in hairless mice
그림 Fig. 17. Effects of 1.0% naringin cream-ointment on UVB-induced wrinkle formation in hairless mice
그림 Fig. 18. Effects of 0.25% naringin cream-ointment on UVB-induced wrinkle formation in hairless mice
그림 Fig. 19. HR-1 hairless male mice dorsal skin on wrinkle photograph.
그림 Fig. 20. Effects of vihicle control on UVB-induced wrinkle photograph in hairless mice
그림 Fig. 21. Effect of 2% PT_iF Extracted Cream Ointment on UVB-induced Wrinkle Photographs in Unhulled Mice
그림 Fig. 22. Effect of 0.5% PT_iF Extract Cream Ointment on UVB-Induced Wrinkle Photographs of Rehmannia Rat
그림 Fig. 23. Effect of 1.0% Naringin cream ointment on UVB-induced wrinkles in hairless mice
그림 Fig. 24. Effect of 0.25% Naringin Cream Ointment on UVB-Induced Wrinkle Photographs of Hairless Rats

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제안 방법

2% and 0.5% of PT_iF, and naringin (1%, 0.25%) – which were made based on vehicle(30% ethanol, 70% polyethylene glycol) - were applied to the skin of the HR-1 mice and the mice were investigated under UVB at a distance of 15 cm for five to ten minutes.
To investigate the wrinkle improvement effect of Naringin and PT_iF extract, UVB was irradiated to the HR-1 mice. After 8 weeks, the tissues were separated from the body, and to figure out the wrinkle formation process due to the collagen decomposition, the expression of COX-2 (A), MMP-2 (B), and M-9PC-2 (MMPC) was examined. And, With the result which came from investigating the the expression of COX-2 (A), MMP-2 (B), and M-9PC-2 (MMPC), and making UVB control group’s COX-2, MMP-2, and MMP-9 gene expression’s RQ value as 1, we analyzed the experimental grou’s value of naringin and PT_iF extract.
And, With the result which came from investigating the the expression of COX-2 (A), MMP-2 (B), and M-9PC-2 (MMPC), and making UVB control group’s COX-2, MMP-2, and MMP-9 gene expression’s RQ value as 1, we analyzed the experimental grou’s value of naringin and PT_iF extract.
As a result of measuring MMP-2 protein production in order to observe the change in skin crease formation which is formed by dermal collagen decomposition due to UVB light aging, the production amount of PT_iF extract and naringin from the skin tissues were analyzed in UVB control group by studying MMP-2 protein expression. As a result, the amount of MMP-2 protein expression.
In this study, in vivo experiment was conducted to measure the effects of naringin and Poncirustrifoliata immature Fruit extracts on animal models such as HR-1 mice which underwent photo-aging. And the effects of them - including suppressing epithelium thickness and increasing collagen fiber contents - were well verified.
UV strength was measured using a HD2102-2 UV meter(Delta OHM, Padova, Italy). UVB irradiation for 8 weeks with vehicle cream-ointment , UVB irradiation for 8 weeks with skin apply of 2% PT_iF-cream ointment(UVB_2% PT+iF), UVB irradiation for 8 weeks with skin apply of 0.5% PT_iF-cream ointment(UVB_0.5% PT+iF), UVB irradiation for 8 weeks with skin apply of 2% naringin-cream ointment(UVB_1% naringin), and UVB irradiation for 8 weeks with skin apply of 0.25% naringin-cream ointment(UVB_0.25% naringin). Dorsal skin Left part and Light part photograph image analysis.

대상 데이터

Poncirustrifoliata immature fruits used in the experiment were purchased from oriental medicine supplier-Omniherb(www.omniherb.com, Korea). After completely drying up the fruits, 100g were grinded as powder form.

데이터처리

The result values of each group were statistically processed using T-test statistics program and a significance test was performed at a level of p<0.05.

성능/효과

As a result of In vivo experiments, it is proven that on PT_iF extracts and naringin do have in a statistically significant decrease on skin creases improvement. Group which were applied 2% PT_iF extract and 1% naringin had significant decrease on epithelial tissue thickness and increase in collagen fiber when compared to UVB control (UVB_vehicles).
As a result of measuring the changes in collagen fiber, it is thought that the breakdown of collagen fibers and wrinkle formation has accelerated, since the strength of M-T dying was significantly decreased in the UVB_control group(Fig. 9A~B), when compared to normal group (Fig. 8A~B), as shown in Fig. 8-13. However, it showed that the amount of collagen fiber was increased by the application of 2% PT_iF extract(Fig.
Furthermore, the test measured collagenase inhibitory activity for PT_iF extract showed low activity of IC50 at about 150 μg/ml and ascorbic acid, the positive control showed low activity of IC50 at 400 μg/ml or above 400 μg/ml.
0. However, UVB_PT_iF 0.50 extract and the application of 0.25% naringin (UVB_Naringin 0.25%) slightly decreased the expression of COX-2, MMP-2, and MMP-9 mRNA when compared to control group (UVB_vehicles), but no statistical significance was shown.
However, the application of 2% PT_iF extract (Fig. 5A–D), 0.5% PT_iF extract (Fig. 4A–D), 1% naringin (Fig. 6A–D) and 0.25% naringin (Fig. 7A– D) has brought a statistically meaningful decrease to epithelial tissue thickness when compared with UV_control, and especially the 1% naringin (Fig .6A–D) has brought a significant decrease, which decreased more than 64.3%.
In order to measure the effect of Naringin and PT_iF extract on skin wrinkle improvement, UVB was irradiated to the skins of HR-1 mice to make wrinkles, and the tissues of the skin were dyed with H&E after 8 weeks to easily find out the test result.
In the experimental group, UVB_PT_iF2.0 Extract (p<0.001), 0UVB_PT_iF 0.5 Extract (p<0.01), 1% naringin (UVB_Narlingin 1.0) (p<0.001), and 0.25% naringin (UVB_Naringin 0.25)(p<0.01) were applied, and the MMP-2 protein expression showed a statistically significant decrease.
Recent studies have shown that PT_IF extracts have anti-alergic action[12], antibacterial action[13], anti-cancer action[14] and melanin-producing inhibiting effects[15]. The results of this experiment show that PT_iF extracts and naringin are proven to be high in electronic contribution capabilities, which results in the fact that they show excellent antioxidant action, and ultimately are thought to be available as an antioxidant. Furthermore, the test measured collagenase inhibitory activity for PT_iF extract showed low activity of IC50 at about 150 μg/ml and ascorbic acid, the positive control showed low activity of IC50 at 400 μg/ml or above 400 μg/ml.

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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