[논문]지르코니아 표면에 부착된 바이오필름에 대한 LED 치솔의 항균효과

박종휴; 김용건; 엄흥식; 이시영; 이재관; 장범석

doi:10.14368/jdras.2019.35.3.160

지르코니아 표면에 부착된 바이오필름에 대한 LED 치솔의 항균효과
Antimicrobial effect of toothbrush with light emitting diode on dental biofilm attached to zirconia surface: an in vitro study 원문보기

Journal of dental rehabilitation and applied science = 구강회복응용과학지, v.35 no.3, 2019년, pp.160 - 169

박종휴 (강릉원주대학교 치과대학 치주과학교실 및 구강과학연구소) , 김용건 (경북대학교 치과대학 치주과학교실) , 엄흥식 (강릉원주대학교 치과대학 치주과학교실 및 구강과학연구소) , 이시영 (강릉원주대학교 치과대학 미생물학 및 면역학교실) , 이재관 (강릉원주대학교 치과대학 치주과학교실 및 구강과학연구소) , 장범석 (강릉원주대학교 치과대학 치주과학교실 및 구강과학연구소)

초록
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목적: 이번 연구의 목적은 정적인 방법과 동적인 방법으로 형성된 지르코니아 표면에 부착된 바이오필름에 대한 LED 칫솔의 항균 효과를 평가하고자 하였다. 연구 재료 및 방법: 구강 바이오필름을 형성하기 위해 직경 12 mm, 두께 2.5 mm의 지르코니아 디스크를 24-well plate(정적 방법)와 Center for Disease Control and Prevention (CDC) biofilm reactor (동적 방법)에 디스크를 넣어 바이오필름을 형성하였다. 디스크는 아무 처치도 하지 않은 대조군, 상용화된 광역학(PDT) 키트, 치솔질(brushing) 단독, LED 치솔군, LED 치솔과 에리스로신을 같이 적용한 군, 이렇게 5개 그룹으로 구성하였다. 각 군별 처치 후, 개별 디스크를 시험관에 넣고 60 초 동안 vortexing하여 세균을 분리한 후, 분리된 세균 용액을 선택 배지를 이용하여 살아있는 세균 수를 확인한 후 실험 방법에 따른 항균 효과를 계측하였고, 주사전자현미경(SEM)을 통하여 세균의 형태 변화를 관찰하였다. 결과: 바이오필름의 형성과 구성비는 동적인 방법과 정적인 방법에 따른 차이는 관찰되지 않았다. 대조군과 실험군간에 세균의 생존률에 유의한 차이가 있었다(P < 0.05). LED 치솔과 에리스로신을 같이 적용한 군에서 가장 높은 항균 효과가 관찰되었다(P < 0.05). 주사전자현미경 사진상에서 광역학치료군과, LED 치솔군, LED 치솔과 에리스로신을 같이 적용한 군은 세균의 형태 변화가 관찰되었으나, 대조군과 치솔질 단독 사용군에서는 세균의 형태 변화가 관찰되지 않았다. 결론: 이번 연구 결과 지르코니아 표면에 부착된 바이오필름을 효과적으로 제거하는 방법으로 LED 치솔과 에리스로신을 같이 적용하는 것이 추천된다.

Abstract ▼ AI-Helper

Purpose: The purpose of this study was to evaluate the antimicrobial effects of a toothbrush with light-emitting diodes (LEDs) on periodontitis-associated dental biofilm attached to a zirconia surface by static and dynamic methods. Materials and Methods: Zirconia disks (12 mm diameter, 2.5 mm thickness) were inserted into a 24-well plate (static method) or inside a Center for Disease Control and Prevention (CDC) biofilm reactor (dynamic method) to form dental biofilms using Streptococcus gordonii and Fusobacterium nucleatum. The disks with biofilm were subdivided into five treatment groups-control, commercial photodynamic therapy (PDT), toothbrush alone (B), brush with LED (BL), and brush with LED+erythrosine (BLE). After treatment, the disks were agitated to detach the bacteria, and the resulting solutions were spread directly on selective agar. The number of viable bacteria and percentage of bacterial reduction were determined from colony counts. Scanning electron microscopy (SEM) was performed to visualize alterations in bacterial morphology. Results: No significant difference in biofilm formation was observed between dynamic and static methods. A significant difference was observed in the number of viable bacteria between the control and all experimental groups (P < 0.05). The percentage of bacterial reduction in the BLE group was significantly higher than in the other treated groups (P < 0.05). SEM revealed damaged bacterial cell walls in the PDT, BL, and BLE groups, but intact cell walls in the control and B groups. Conclusion: The findings suggest that an LED toothbrush with erythrosine is more effective than other treatments in reducing the viability of periodontitis-associated bacteria attached to zirconia in vitro.

주제어

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문제 정의

This study was designed to evaluate the antimicrobial effects of LED toothbrushes on periodontitisassociated dental biofilm attached to zirconia surfaces by CDC biofilm reactor in vitro. We used S.

제안 방법

After 5 days of incubation, the zirconia disks with multi-species biofilms formed using the dynamic and static methods were divided into five groups, comprising eight disks each (Table 1): negative control; commercial photodynamic therapy (PDT; Periowave system, Ondine Biomedical Inc., Vancouver, Canada) as positive control; brushing with toothbrush (B; Smart E-care, AinA Co., Ltd., Daegu, South Korea) only; brushing with an LED light (BL): and brushing with an LED light and erythrosine (BLE).
Therefore, the present study aimed to evaluate the antimicrobial effects of the LED toothbrush on periodontitis-associated dental biofilm attached to a zirconia surface prepared by static and dynamic methods.
nucleatum is the most abundant gram-negative anaerobic bacteria in biofilms from healthy gingiva and increases with periodontal disease progression. This study evaluated the antimicrobial effect of an LED toothbrush on the initial biofilm that could be formed on a zirconia abutment and crown, using S. gordonii and F. nucleatum to reproduce the conditions observed in peri-implant mucositis, an early peri-implant disease.

대상 데이터

, Daegu, South Korea) only; brushing with an LED light (BL): and brushing with an LED light and erythrosine (BLE). The LED light comprised one red LED (630 nm, 44 mW), two blue LEDs (465 nm, 64 mW), and one white LED (550 nm, 64 mW). The disks from the PDT group were placed in methylene blue (1 mL, 100 μg/mL) for 60 s, followed by irradiation (670 nm, 160 mW) with the diode laser for 60 s.
This study was supported by the Scientific Research of Gangneung-Wonju National University Dental Hospital (SR1704). The LED toothbrush used in the experiment was provided by AinA (Daegu, South Korea). Zirconia discs were supplied by HASS Corporation (Gangneung, Korea).
The fixed samples were washed 3× with PBS for 10 min each and dehydrated for 30 min in graded ethanol solutions (30%, 50%, 70%, 90%, and 100%).
This study was supported by the Scientific Research of Gangneung-Wonju National University Dental Hospital (SR1704). The LED toothbrush used in the experiment was provided by AinA (Daegu, South Korea).
Zirconia disks (HASS Corporation, Gangneung, Korea) measuring 12 mm in diameter and 2.5 mm in thickness were manufactured. One side of the zirconia disk was covered with a putty-type hydrophilic vinyl polysiloxane material (Eli-dent Group S.

이론/모형

1. Scanning electron microscopy images of attachment on zirconia surface according to culture method. (A, C) Biofilm in well plate (static method), (B, D) Biofilm in CDC biofilm reactor (dynamic method).

성능/효과

²⁸ However, both methods are useful for mimicking periodontitis-associated oral biofilms. In this study, the mean log CFU/mL of biofilms in the dynamic method group were lower than those in the static group, but no significant difference was observed in the level of cell growth between the two methods. Our results concur with the previous study, even though different periodontal bacteria were used in the two studies.

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저자의 다른 논문 :

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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