[논문]α-Bisabolol을 함유한 PIT Nanoemulsion의 최적화 및 피부흡수연구

김희주; 윤경섭

doi:10.12925/jkocs.2020.37.6.1738

α-Bisabolol을 함유한 PIT Nanoemulsion의 최적화 및 피부흡수연구
Study on Optimization and Skin Permeation of PIT Nanoemulsion Containing α-Bisabolol 원문보기

Journal of the Korean Applied Science and Technology = 한국응용과학기술학회지, v.37 no.6, 2020년, pp.1738 - 1751

김희주 (제주대학교 화학?코스메틱스학과,) , 윤경섭 (제주대학교 화학?코스메틱스학과,)

초록
AI-Helper

피부는 표피, 진피, 피하지방의 세 부분으로 나누어져 있으며, 표피의 가장 윗부분에 존재하는 각질층은 약물의 피부 전달을 방해하는 장벽 역할을 한다. 나노에멀젼은 특유의 작은 입자크기 때문에 세포간 지질을 통과하여 약물의 피부전달에 효과적이라고 알려져 있다. 본 연구에서는 α-bisabolol의 효과적인 피부흡수를 위해 반응표면분석법(response surface methodology, RSM)을 이용하여 상반전온도(phase inversion temperature, PIT) 유화법으로 제조한 α-bisabolol을 함유한 나노에멀젼을 최적화하였다. 예비실험으로 25-2 일부요인배치법과 23 요인배치법이 수행되었다. 요인배치법의 결과를 바탕으로 계면활성제(6.3~12.6%), 보조계면활성제(5.2~7.8%) 및 α-bisabolol (0.5~5.0%) 함량을 인자로 하고 반응 변수를 나노에멀젼의 입자크기로 하는 Box-Behnken design을 수행하였다. RSM 결과에 따라 PIT 나노에멀젼 최적화를 수행하였고, 그 결과 최적의 나노에멀젼 처방 조건은 계면활성제 함량 10.4%, 보조계면활성제 함량 6.3%, α-bisabolol 함량 5.0%로 예측되었다. 피부흡수시험 결과 PIT 나노에멀젼의 최종 피부흡수율은 35.11±1.01%, 대조군인 일반에멀젼의 최종 피부흡수율은 28.25±1.69%로 PIT 나노에멀젼의 피부흡수율이 더 우수함을 확인하였다.

Abstract ▼ AI-Helper

The skin is divided into three parts: the epidermis, the dermis, and the subcutaneous fat, and the stratum corneum, which is located at the top of the epidermis, acts as a barrier that prevents drug delivery. Nanoemulsions are known to be effective in transdermal delivery of drugs through intercellular lipids because of their unique small particle size. In this study, phase inversion temperature (PIT) nanoemulsion containing α-bisabolol was optimized using response surface methodology (RSM) for effective skin absorption of α-bisabolol. As a preliminary experiment, the 25-2 fractional factorial design method and the 23 full factorial design method were performed. Box-Behnken design was performed based on the results of the factorial design method. The content of surfactant (6.3~12.6%), co-surfactant (5.2~7.8%) and α-bisabolol (0.5~5.0%) were used as factors, and the dependent variable was the particle size of the nanoemulsion. PIT nanoemulsion optimization was performed according to the RSM results, and as a result, the optimal nanoemulsion formulation conditions were predicted to be 10.4% surfactant content, 6.3% co-surfactant content, and 5.0% α-bisabolol content. As a result of the skin absorption test, the final skin absorption rate of the PIT nanoemulsion was 35.11±1.01%, and the final skin absorption rate of the general emulsion as a control was 28.25±1.69%, confirming that the skin absorption rate of the PIT nanoemulsion was better.

주제어

표/그림 (23)

그림 Fig. 1. Molecular structure of α-bisabolol.
표 Table 1. List of used raw material
그림 Fig. 2. Experimental matrix for the 2³ full factorial design.
$Table 2. Experimental conditions for the 2<sup>5-2</sup> fractional factorial design$ 표 Table 2. Experimental conditions for the 2^5-2 fractional factorial design
표 Table 3. Experimental conditions for the 2³ full factorial design
표 Table 4. Independent variables range of PIT nanoemulsion optimization experiments
표 Table 5. Condition of HPLC analysis
$Table 6. Experimental matrix for the fractional factorial design and particle size$ 표 Table 6. Experimental matrix for the fractional factorial design and particle size
$Table 7. ANOVA of the fractional factorial design$ 표 Table 7. ANOVA of the fractional factorial design
$Fig. 3. Main effect plot for particle size obtained from the fractional factorial design.$ 그림 Fig. 3. Main effect plot for particle size obtained from the fractional factorial design.
표 Table 8. Experimental matrix for the fulll factorial design and particle size
표 Table 9. ANOVA of the fulll factorial design
그림 Fig. 4. Main effects plot for particle size obtained from the 2³ full factorial design.
그림 Fig. 5. Interaction effects plot for particle size obtained from the 2³ full factorial design.
표 Table 10. Box-Behnken design experiments for PIT nanoemulsion optimization
표 Table 11. ANOVA for the model
그림 Fig. 6. 3D surface model graph. (a): particle size of nanoemulsion depending on surfactant and co-surfactant; (b): particle size of nanoemulsion depending on surfactant and α-bisabolol; (c): particle size of nanoemulsion depending on co-surfactant and α-bisabolol.
표 Table 12. Optimization condition
표 Table 13. Confirmation
표 Table 14. Characteristic of samples
그림 Fig. 7. Samples for in-vitro transdermal permeation experiment.
그림 Fig. 8. Permeation profiles of α-bisabolol through Strat-M membrane from the emulsions (mean±SD, n=3).
그림 Fig. 9. Permeation profiles of α-bisabolol through Strat-M membrane from the emulsions (mean±SD, n=3).

참고문헌 (15)

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상세보기

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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