[논문]Symptomatology of Citrus mosaic sadwavirus (CiMV) in Some Citrus Cultivars and Effect of CiMV Infection on Citrus Fruit Quality

Hyun, Jae Wook; Hwang, Rok Yeon; Choi, Cheol Woo; Jung, Kyung Eun; Han, Seung Gab

doi:10.5423/ppj.nt.07.2019.0192

[국내논문] Symptomatology of Citrus mosaic sadwavirus (CiMV) in Some Citrus Cultivars and Effect of CiMV Infection on Citrus Fruit Quality 원문보기

The plant pathology journal, v.36 no.1, 2020년, pp.106 - 110

Hyun, Jae Wook (Citrus Research Institute, National Institute of Horticultural & Herbal Science, RDA) , Hwang, Rok Yeon (Citrus Research Institute, National Institute of Horticultural & Herbal Science, RDA) , Choi, Cheol Woo (Citrus Research Institute, National Institute of Horticultural & Herbal Science, RDA) , Jung, Kyung Eun (Citrus Research Institute, National Institute of Horticultural & Herbal Science, RDA) , Han, Seung Gab (Citrus Research Institute, National Institute of Horticultural & Herbal Science, RDA)

Abstract ▼ AI-Helper

Citrus mosaic sadwavirus (CiMV) is a closely related virus with the Satsuma dwarf virus (SDV) along with Navel orange infectious mottling virus (NIMV), Natsudaidai dwarf virus (NDV), and Hyugagatsu virus (HV). The present study found that the typical symptoms of CiMV-infected citrus fruits include the appearance of dark blue speckles or ringspots on fruit rinds and the browning of oil glands in the spots as rind coloring began. As rind coloring progressed, the spots gradually faded, whereas the browning of the oil glands worsened to the point that the tissues surrounding the oil glands became necrotic. In very early satsuma mandarins (Citrus unshiu 'Miyamoto Wase') and 'Setoka' cultivar (C. hybrid 'Setoka') of late-maturity citrus, the symptomatic fruits were eventually dropped. And in early satsuma mandarin (C. unshiu 'Miyakawa Wase'), the peel hardness of the virus-infected fruit (1,618.3 ± 305.5, g-force) was more than twice as hard as that of the healthy fruit (636.5 ± 39.1, g-force). The ratio of flesh weight to total fruit weight was higher for the healthy fruit (77.3 ± 1.7%) than for the infected fruit (70.7 ± 0.6) and peel puffing was more severe in the infected fruit (2.9 ± 0.4 mm) than in the healthy fruit (0.9 ± 0.2 mm). The soluble solids content in infected citrus fruits was less values than the healthy fruit by 0.5-1.5 °Brix. These findings reveal that CiMV infection on citrus trees reduces the fruit quality of citrus.

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표/그림 (7)

그림 Fig. 1. Typical symptoms shown on citrus cultivars infected with Citrus mosaic sadwavirus (CiMV).
표 Table 1. Virus isolates and detection of citrus viruses
그림 Fig. 2. Simultaneous detection of citrus viruses using multiplex PCR.
그림 Fig. 3. Uniplex PCR for detection of of Citrus mosaic sadwavirus (CiMV) using Sadwa primer set.
표 Table 2. Primers used to detect the 4 citrus viruses in uniplex or multiplex PCR
표 Table 3. Effect of Citrus mosaic sadwavirus (CiMV) infection on the quality of citrus fruit
표 Table 4. Effect of Citrus mosaic sadwavirus (CiMV) on the sugar and acid contents of citrus fruit

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,1999). Therefore, this study was carried out to investigateCiMV symptoms according to citrus cultivars and the effectsof CiMV on quality of citrus fruit in Korea.

제안 방법

The scionsfrom each of the satsuma mandarin trees were grafted ontotrifoliate orange rootstock, maintained in a greenhouse andused to detect viruses. For diagnosis of CTV, CTLV, andSDV/CiMV, multiplex PCR was performed using LiliFCitrus Virus Muliplex PCR Kit (catalog No. IP11075, iNtRONBiotechnology Inc., Seongnam, Korea) with CTVPo,PP2-3/4, CTLV-2013, and actin primer sets (Table 2,Fig. 2). And SDV and CiMV was specifically detected byuniplex PCR with SDV-2014 and Sadaw primer sets (Table2), respectively, using Accupower Hotstart PCR pemimix(catalog No.

대상 데이터

1). The five isolates, SM-1, SM-26, Jung-CiMV-3, Nam-CiMV, and Sehwa, were collected from each of early satsumamandarin trees showing CiMV typical symptoms inNamwon, Jeju to assay fruit quality (Table 1). The scionsfrom each of the satsuma mandarin trees were grafted ontotrifoliate orange rootstock, maintained in a greenhouse andused to detect viruses.

성능/효과

, 2017). And it is not to be said that CTV did not affectthe expression of CiMV at all because the most CTVwere mild strains, but since the typical symptoms of CTVdid not appear but only the typical symptoms of CiMV,the symptoms of trees investigated in this study might bemainly induced by CiMV infection. However, even if CTVis mild strain, the possibility of affecting the virus severityby coinfection with CiMV cannot be ruled out.
26%, respectively (Table 4). Thus, theresults of the present study confirmed that CiMV reducesthe commercial quality of citrus fruits and affects internalquality characteristics, such as SSC, even though the treeswere coinfected with CiMV and CTV because these CTVisolates were mild strain by enzyme-linked immunosorbentassay using MCA-13 antibody (data not presented).Practically, though CTV was detected in 72.

참고문헌 (11)

Hyun JW Hwang RY Jung KE 2017 Development of multiplex PCR for simultaneous detection of citrus viruses and the incidence of citrus viral diseases in late-maturity citrus trees in Jeju Island Plant Pathol J 33 307 317 10.5423/PPJ.OA.10.2016.0207 28592949

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Hyun JW Min BK Kim HJ Lee PH Kim KS 2009 Simultaneous detection of seven citrus viroids from citrus plants by multiplex real-time PCR 2009 KSPP Annual Meeting and International Conference 204 The Korean Society of Plant Pathology Seoul, Korea
Imada J Tanaka H Narisawa N 1980 The effect of satsuma dwarf virus and citrus mosaic virus on the growth of citrus trees Bull Fruit Trees Res Stn E 3 75 82 (in Japanese)
Ito T Ieki H Ozaki K 2002 Simultaneous detection of six citrus viroids and Apple stem grooving virus from citrus plants by multiplex reverse transcription polymerase chain reaction J Virol Methods 106 235 239 10.1016/S0166-0934(02)00147-7 12393154

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Ito T Shiotani H Iwanami T Ozaki K Muramoto K 2007 Genetic diversity and a heterogeneous population of Citrus mosaic virus within a single citrus tree J Gen Plant Pathol 73 147 151 10.1007/s10327-006-0329-1

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Ito T Iwanami T Ieki H Shimomura K Shimizu S Ito T 2004 A new virus related to Satsuma dwarf virus: the nucleotide sequence of the 3′-terminal regions of Hyuganatsu virus RNAs 1 and 2 Arch Virol 149 1459 1465 10.1007/s00705-003-0284-5 15221545

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Iwanami T 2010 Properties and control of Satsuma dwarf virus JARQ 44 1 6 10.6090/jarq.44.1

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Iwanami T Koizumi M 2000 Satsuma dwarf virus group Compendium of citrus diseases Timmer LW Garnsey SM Graham JH 59 American Phytopathological Society St. Paul, MN, USA
Iwanami T Kondo Y Karasev AV 1999 Nucleotide sequences and taxonomy of satsuma dwarf virus J Gen Virol 80 793 797 10.1099/0022-1317-80-3-793 10092021

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Iwanami T Kondo Y Kobayashi M Han SS Karasev AV 2001 Sequence diversity and interrelationships among isolates of satsuma dwarf-related viruses Arch Virol 146 807 813 10.1007/s007050170149 11402866

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Korkmaz S Garnsey SM Chagas CM Derrick KS Barthe GA Iwanami T Koizumi M Miyakawa T Ito T Lee RF Bar-Joseph M da Graca JV Ahlawat YS Moreno P 2000 Graft-transmissible, systemic diseases Compendium of citrus diseases Timmer LW Garnsey SM Graham JH 46 48 American Phytopathological Society St. Paul, MN, USA

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DOI : 10.5423/PPJ.NT.07.2019.0192 [무료]
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[논문] Occurrence of Citrus mosaic virus on Satsma mndarin (Citrus unshiu) in Jeju island.

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