Lee, Jin Woo
(Research & Development Center, Richwood Pharmaceuticals)
,
Park, Se Hoon
(Research & Development Center, Richwood Pharmaceuticals)
,
Jegal, Chang Min
(Research & Development Center, Richwood Pharmaceuticals)
,
Choi, Keun Young
(Research & Development Center, Richwood Pharmaceuticals)
,
Jung, Hye Young
(Research & Development Center, Richwood Pharmaceuticals)
,
Choi, Jung A
(Research & Development Center, Richwood Pharmaceuticals)
,
Lee, Chan Kyu
(Research & Development Center, Richwood Pharmaceuticals)
,
Kim, Ho Kyong
(Research & Development Center, Richwood Pharmaceuticals)
,
Lee, Jung Suk
(Research & Development Center, Richwood Pharmaceuticals)
,
Lee, Il Kyun
(Research & Development Center, Richwood Pharmaceuticals)
In this study, we investigated the chemical profile and effects of RW0117 (Artemisia argyi 65 .5 % ethanol extract) on gastric lesions in rats. We optimized and validated a method to obtain the chemical profile of RW0117. We then investigated the antioxidant and anti-inflammatory effects in vitro, a...
In this study, we investigated the chemical profile and effects of RW0117 (Artemisia argyi 65 .5 % ethanol extract) on gastric lesions in rats. We optimized and validated a method to obtain the chemical profile of RW0117. We then investigated the antioxidant and anti-inflammatory effects in vitro, and the protective effects on gastric lesions in vivo. The IC50 of 2,2-diphenyl-1-picrylhydrazyl free radical scavenging considering the antioxidant effects of RW0117 was 166.55 ㎍/mL, and the IC50 of nitric oxide scavenging considering the anti-inflammatory effects was 41.16 ㎍/mL. Oral administration of RW0117 at lower concentrations (25, 50, 100 mg/kg) had similar or greater effects than the daily intake conversion concentration (115mg/kg) of a health functional food (Avexol®) in the acetic acid-induced ulcer and the ethanol-induced gastric injury rat models. In addition, oral administration of RW0117 increased the expression of prostaglandin E2, which enhances the protective effect in the gastric mucosa in the ethanol-induced gastric injury rat model. These results suggest that RW0117 may have potential therapeutic uses in the protection of the gastric mucosa.
In this study, we investigated the chemical profile and effects of RW0117 (Artemisia argyi 65 .5 % ethanol extract) on gastric lesions in rats. We optimized and validated a method to obtain the chemical profile of RW0117. We then investigated the antioxidant and anti-inflammatory effects in vitro, and the protective effects on gastric lesions in vivo. The IC50 of 2,2-diphenyl-1-picrylhydrazyl free radical scavenging considering the antioxidant effects of RW0117 was 166.55 ㎍/mL, and the IC50 of nitric oxide scavenging considering the anti-inflammatory effects was 41.16 ㎍/mL. Oral administration of RW0117 at lower concentrations (25, 50, 100 mg/kg) had similar or greater effects than the daily intake conversion concentration (115mg/kg) of a health functional food (Avexol®) in the acetic acid-induced ulcer and the ethanol-induced gastric injury rat models. In addition, oral administration of RW0117 increased the expression of prostaglandin E2, which enhances the protective effect in the gastric mucosa in the ethanol-induced gastric injury rat model. These results suggest that RW0117 may have potential therapeutic uses in the protection of the gastric mucosa.
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가설 설정
16µg/mL) as shown by the NO assay. Therefore, based on the observed antioxidant and anti-inflammatory effects, we hypothesized that treatment with RW0117 would be effective against gastritis and ulcers, and would protect the gastric mucosa. Gastric ulcers occur when the submucosal layer is damaged as a result of gastritis caused by damage to the gastric mucous membrane.
제안 방법
of analytical grade. High performance liquid chromatography analysis was performed using an Agilent 1260 Infinity system (Agilent Technologies; Santa Clara, CA, USA) with an Inno Column (5μm, 4.6×250mm) from Young Jin Biochrom Co., Ltd (Sungnam, Korea). The compounds were isolated using 321 pumps and a UV/Vis-151 detector from Gilson (Middleton, WI, USA) and a Luna C18 (2) (10μm, 21.
The aim of our study was to investigate the antioxidant and antiinflammatory effects of extraction powder from A. argyi (RW0117), and to confirm the protective effects on the gastric mucosal barrier using acetic acid-induced gastric ulcer and ethanol-induced acute gastric damage models in rat. Furthermore, we suggest the stable qualify assurance method by establishing the chemical profile of RW0117.
대상 데이터
Louis, MI, USA). Dimethyl sulfoxide (DMSO) and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) were purchased from Duchefa Biochemie (Haarlem, North Holland, Netherland). Fetal bovine serum (FBS) was purchased from Thermo Fisher Scientific (Waltham, MA, USA) and Dulbecco's modified Eagle medium (DMEM) was purchased from WELLGENE (Kyungsan, Korea).
Methylcellulose (MC), acetic acid, saline, formalin solution, ethanol (EtOH), butylated hydroxyanisole (BHA), 1, 1-diphenyl-2-picrylhydrazyl (DPPH), NG-monomethyl-L-arginine (L-NMMA), N-(1-naphthyl)-ethylenediamine dihydrochloride (NED), lipopolysaccharide (LPS), and sodium nitrite (NaNO2) were purchased from Sigma-Aldrich Co. (St. Louis, MI, USA). Dimethyl sulfoxide (DMSO) and 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) were purchased from Duchefa Biochemie (Haarlem, North Holland, Netherland).
Fetal bovine serum (FBS) was purchased from Thermo Fisher Scientific (Waltham, MA, USA) and Dulbecco's modified Eagle medium (DMEM) was purchased from WELLGENE (Kyungsan, Korea). Sulfanilamide, methanol (MeOH), and ortho-Phosphoric acid were purchased from Merck (Darmstadt, Germany). The prostaglandin E2 (PGE2) ELISA Kit (#514010, Cayman, USA), 1.
, Ltd (Sungnam, Korea). The compounds were isolated using 321 pumps and a UV/Vis-151 detector from Gilson (Middleton, WI, USA) and a Luna C18 (2) (10μm, 21.20×250mm, 10×250 mm) column (Torrance, CA, USA), and were identified by applying Digital ADVANCE Ⅲ 400 and Thermo Scientific DFS from Bruker co. (Billerica, MA, USA). Pure compounds 1–6 were purchased from Sigma-Aldrich (St.
Each sub-fraction was purified by flash column chromatography and refined to obtain six compounds using HPLC. These were identified as: 3, 4-di-caffeoylquinic acid (1), 3, 5-di-caffeoylquinic acid (2), 4, 5-di-caffeoylquinic acid (3), hispidulin (4), jaceosidin (5), eupatilin (6) by comparing 1H-, 13C-NMR and MS spectral data with those in the literature (Table 1).
이론/모형
Statistical analysisData were analyzed using the Mann–Whitney method. All analyses were conducted using SPSS software (Version 19.
성능/효과
24 Therefore, in this study, the enzymatic activity of PGE2 was involved in the regeneration and protection of the mucus layer on gastric mucosal tissue. Determination of PGE2 enzymatic activity on gastric mucosa cells revealed that this was significantly higher in the Abexol (53.3%), RW0117 25㎎/㎏ (47.7%), and 50㎎/㎏ (38.75%) groups compared with the EtOH-induced stomach damage group (p<0.01; Fig. 9, Table 9). In the present study, we showed that it is possible to maintain quality and to guarantee efficacy by establishing a chemical profile showing the composition and ratio of major compound.
22 Therefore, we anticipated that reducing the generation of free radicals and NO would be beneficial for the treatment of gastritis and ulcers. The DPPH assay revealed a concentration-dependent increase in the free radical scavenging activity of RW0117, with values of 9.47, 21.28, 25.9, 42.87, and 53.58% obtained with 12.5, 25, 50, 100, and 200µg/mL, respectively (Table 4, Fig. 2). In the mouse macrophage cell line, RAW 264.
참고문헌 (24)
Kang, M. H.; Lee, J. H.; Lee, Y. S.; Son, K. H.; Lee, D. H.; Kim, Y. S.; Kang, S. S.; Bang, H. C.; Jeong, C. S. Yakhak Hoeji 2007, 51, 68-74.
Joo, Y. E.; Park, H. K.; Myung, D. S.; Baik, G. H.; Shin, J. E.; Seo, G. S.; Kim, G. H.; Kim, H. U.; Kim, H. Y.; Cho, S. I.; Kim, N. Gut. Liver 2013, 7, 303-310.
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