Nguyen, Thi Thu
(College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University)
,
Vu, Thi Oanh
(College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University)
,
Cao, Thao Quyen
(College of Pharmacy, Drug Research and Development Center, Daegu Catholic University)
,
Min, Byung Sun
(College of Pharmacy, Drug Research and Development Center, Daegu Catholic University)
,
Kim, Jeong Ah
(College of Pharmacy, Research Institute of Pharmaceutical Sciences, Kyungpook National University)
Artemisia capillaris Thunb. (Compositae) is a traditional medicinal plant with various pharmacological activities. To elucidate new anti-allergic and anti-inflammatory constituents, the aerial parts of A. capillaries were investigated to afford a new compound, (6E,8E)-6-methylundeca-6,8-diene-2,5,10...
Artemisia capillaris Thunb. (Compositae) is a traditional medicinal plant with various pharmacological activities. To elucidate new anti-allergic and anti-inflammatory constituents, the aerial parts of A. capillaries were investigated to afford a new compound, (6E,8E)-6-methylundeca-6,8-diene-2,5,10-trione (17) together with 19 known compounds (1 - 16, 18 - 20). The structures of these compounds were determined by extensive spectroscopic analyses including 1D, 2D NMR, HREIMS, and optical rotation [α]D. The absolute configuration of compound 2 was determined to be S form for the first time. All isolates (1 - 20) were tested their inhibitory effects on interleukin 2 (IL-2) expression in T cells and NO production in lipopolysaccharide (LPS)-stimulated RAW246.7. Among them, compounds 10, 11, 19, and 20 reduced IL-2 expression in a dose-dependent manner. In addition, compound 10 also inhibited NO production with an IC50 value of 37.3 ± 0.4 μM.
Artemisia capillaris Thunb. (Compositae) is a traditional medicinal plant with various pharmacological activities. To elucidate new anti-allergic and anti-inflammatory constituents, the aerial parts of A. capillaries were investigated to afford a new compound, (6E,8E)-6-methylundeca-6,8-diene-2,5,10-trione (17) together with 19 known compounds (1 - 16, 18 - 20). The structures of these compounds were determined by extensive spectroscopic analyses including 1D, 2D NMR, HREIMS, and optical rotation [α]D. The absolute configuration of compound 2 was determined to be S form for the first time. All isolates (1 - 20) were tested their inhibitory effects on interleukin 2 (IL-2) expression in T cells and NO production in lipopolysaccharide (LPS)-stimulated RAW246.7. Among them, compounds 10, 11, 19, and 20 reduced IL-2 expression in a dose-dependent manner. In addition, compound 10 also inhibited NO production with an IC50 value of 37.3 ± 0.4 μM.
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가설 설정
a) The inhibitory effects are represented as the molar concentration (μM) giving 50% inhibition (IC50) relative to the vehicle control.
b) 13C-NMR measured at 125 MHz.
). b) Positive control for NO production. c) Cytotoxicity
제안 방법
Each PCR reaction was performed using the following conditions: 94 oC 30 s, 60 oC 30 s, 72oC 30 s, plate read (detection of fluorescent product) for 40 cycles followed by 7 min extension at 72 oC 17.
As a result, none of the isolated compounds significantly affected the viability of activated Jurkat T-cells, even at a concentration of 100 μM (data not shown). Next, to confirm whether compounds 10, 11, 19, and 20 regulate T cell activity, cells were pre-incubated with 10, 11, 19, and 20 at various concentrations for 30 min before treatment with PMA/A23187. After 6 h, IL-2 mRNA levels were measured by PCR.
In the previous studies, chrysin has been demonstrated to be a potent inhibitor of aromatase and of human immunodeficiency virus activation and possessed antiinflammatory and anti-oxidant effects.38This study further confirmed the anti-inflammatory effects of chrysin through inhibition of NO production and suggested a new candidate for the development of anti-inflammatory agents from natural sources.
대상 데이터
A new compound (17) along with nineteen known compounds (1 - 16, 18 - 20) were isolated by various column chromatographies using silica gel, reverse phase gel, HPLC, and TLC from the MC soluble fraction of A. capillaris.
In this study, compounds (1 - 20) were tested to inhibit IL-2 production in Jurkat T cells stimulated with PMA (200 nM) and A23187 (1 μM).
capillaris were purchased from Wansan traditional market in Jeonju (July 2016) and identified by Professor Byung Sun Min at Daegu Catholic University. It has been deposited at Pharmacognosy laboratory (voucher no. 20B-AC) in College of Pharmacy, Kyungpook National University, Korea.
이론/모형
The absorbance at 540 nm was determined with a microplate reader (Molecular Devices, Emax, Sunnyvale, CA, USA), and the absorption coefficient was calibrated by using a sodium nitrite (NaNO2) solution standard. Cell viability was measured with an MTT-based colorimetric assay. For this experiment, celastrol was used as a positive control.
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