Khan, Muhammad Imran
(Department of Civil and Environmental Engineering, College of Engineering, Yonsei University)
,
Yoo, Keunje
(Department of Civil and Environmental Engineering, College of Engineering, Yonsei University)
,
Kim, Seonghoon
(Department of Civil and Environmental Engineering, College of Engineering, Yonsei University)
,
Cheema, Sardar Alam
(Department of Agronomy, University of Agriculture)
,
Bashir, Safdar
(Institute of Soil and Environmental Sciences, University of Agriculture)
,
Park, Joonhong
(Department of Civil and Environmental Engineering, College of Engineering, Yonsei University)
In the present study, an anaerobic microbial consortium for the degradation of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) was selectively enriched with the co-addition of RDX and starch under nitrogen-deficient conditions. Microbial growth and anaerobic RDX biodegradation were effectively enhance...
In the present study, an anaerobic microbial consortium for the degradation of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) was selectively enriched with the co-addition of RDX and starch under nitrogen-deficient conditions. Microbial growth and anaerobic RDX biodegradation were effectively enhanced by the co-addition of RDX and starch, which resulted in increased RDX biotransformation to nitroso derivatives at a greater specific degradation rate than those for previously reported anaerobic RDX-degrading bacteria (isolates). The accumulation of the most toxic RDX degradation intermediate (MNX [hexahydro-1-nitroso-3,5-dinitro-1,3,5-triazine]) was significantly reduced by starch addition, suggesting improved RDX detoxification by the co-addition of RDX and starch. The subsequent MiSeq sequencing that targeted the bacterial 16S rRNA gene revealed that the Sporolactobacillus, Clostridium, and Paenibacillus populations were involved in the enhanced anaerobic RDX degradation. These results suggest that these three bacterial populations are important for anaerobic RDX degradation and detoxification. The findings from this work imply that the Sporolactobacillus, Clostridium, and Paenibacillus dominant microbial consortium may be valuable for the development of bioremediation resources for RDX-contaminated environments.
In the present study, an anaerobic microbial consortium for the degradation of hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) was selectively enriched with the co-addition of RDX and starch under nitrogen-deficient conditions. Microbial growth and anaerobic RDX biodegradation were effectively enhanced by the co-addition of RDX and starch, which resulted in increased RDX biotransformation to nitroso derivatives at a greater specific degradation rate than those for previously reported anaerobic RDX-degrading bacteria (isolates). The accumulation of the most toxic RDX degradation intermediate (MNX [hexahydro-1-nitroso-3,5-dinitro-1,3,5-triazine]) was significantly reduced by starch addition, suggesting improved RDX detoxification by the co-addition of RDX and starch. The subsequent MiSeq sequencing that targeted the bacterial 16S rRNA gene revealed that the Sporolactobacillus, Clostridium, and Paenibacillus populations were involved in the enhanced anaerobic RDX degradation. These results suggest that these three bacterial populations are important for anaerobic RDX degradation and detoxification. The findings from this work imply that the Sporolactobacillus, Clostridium, and Paenibacillus dominant microbial consortium may be valuable for the development of bioremediation resources for RDX-contaminated environments.
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제안 방법
Table 1. Comparison of anaerobic RDX biodegradation rates between the known RDX-degrading isolates and IK consortium from this work.
대상 데이터
5°C in the dark without shaking. Periodic samples were taken for analysis using disposable syringes and 21-gauge needles.
[34]. The representative nucleotide sequences obtained in this study were deposited in the GenBank under the accession numbers KR779830- KR779845.
성능/효과
Furthermore, the detoxification of RDX by the developed anaerobic consortium was improved by the addition of starch. Taken together with our previous study of starch-enhanced aerobic RDX detoxification, the findings of this study suggest that, regardless of oxygen conditions, the addition of starch could be an effective way to stimulate explosive biodegradation for improving explosive detoxification.
The 16S rRNA sequences of the Sporolactobacillus (RCS1–RCS5), Clostridium (RCS6–RCS10) and Paenibacillus (RCS11–RCS12) members, the relative abundances of which significantly increased during the enrichment with RDX, were found to be phylogenetically distant from the known anaerobic RDX-degrading bacteria (more than 5% dissimilarity).
The results suggested that some members of Sporolactobacillus (RCS1–RCS5), Clostridium (RCS6–RCS10) and Paenibacillus (RCS11–RCS12) may be novel anaerobic RDX-degrading bacteria.
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