[논문]p-Anisaldehyde Exerts Its Antifungal Activity Against Penicillium digitatum and Penicillium italicum by Disrupting the Cell Wall Integrity and Membrane Permeability

Che, Jinxin; Chen, Xiumei; Ouyang, Qiuli; Tao, Nengguo

doi:10.4014/jmb.1911.11032

p-Anisaldehyde Exerts Its Antifungal Activity Against Penicillium digitatum and Penicillium italicum by Disrupting the Cell Wall Integrity and Membrane Permeability 원문보기

Journal of microbiology and biotechnology, v.30 no.6, 2020년, pp.878 - 884

Che, Jinxin (School of Chemical Engineering, Xiangtan University) , Chen, Xiumei (School of Chemical Engineering, Xiangtan University) , Ouyang, Qiuli (School of Chemical Engineering, Xiangtan University) , Tao, Nengguo (School of Chemical Engineering, Xiangtan University)

Abstract ▼ AI-Helper

Penicillium digitatum and P. italicum are the two important postharvest pathogens in citrus, causing about 90% of the total loss of citrus fruit during storage and transportation. Natural fungicides such as essential oils have been widely used instead of chemical fungicides for preventing and controlling postharvest diseases. In this research, p-anisaldehyde exhibited a strong inhibitory effect on P. digitatum and P. italicum, with the minimum inhibitory concentration and minimum fungicidal concentration values of both being 2.00 μl/ml. Additionally, p-anisaldehyde visibly inhibited both the green mold and blue mold development of citrus fruits inoculated with P. digitatum and P. italicum. The mycelia morphologies of these pathogens were greatly altered, and the membrane permeability and cell wall integrity of mycelia were severely disrupted under p-anisaldehyde treatment. These results suggest that the antifungal activity of p-anisaldehyde against P. digitatum and P. italicum can be attributed to the disruption of the cell wall integrity.

주제어

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문제 정의

The purpose of this study was to investigate the antifungal efficiency of p-anisaldehyde on the mycelial growth of P. digitatum and P. italicum and to reveal the possible mechanism involved.

제안 방법

The membrane permeability of mycelia was analyzed by the propidium iodide (PI) staining method [19]. Twoday-old mycelia from PDB were collected and treated with p-anisaldehyde (0, 1/2 MIC, and MIC) for 30, 60, and 120 min. The treated mycelia were dyed with 10 μg/ml PI for 5 min at 30°C, then centrifuged at 4,000 ×g for 15 min after being washed three times with PBS.
The membrane permeability of mycelia was analyzed by the propidium iodide (PI) staining method [19]. Twoday-old mycelia from PDB were collected and treated with p-anisaldehyde (0, 1/2 MIC, and MIC) for 30, 60, and 120 min. The treated mycelia were dyed with 10 μg/ml PI for 5 min at 30°C, then centrifuged at 4,000 ×g for 15 min after being washed three times with PBS.

대상 데이터

, China). All of the tests were performed in triplicate.
The colony zone diameters were measured per 24 h. All tests were performed in triplicate.

데이터처리

The data were expressed as the mean ± SD (standard deviation) by measuring three independent replicates and analyzing them by the least significant difference (LSD) test of variance (ANOVA).

이론/모형

The membrane permeability of mycelia was analyzed by the propidium iodide (PI) staining method [19]. Twoday-old mycelia from PDB were collected and treated with p-anisaldehyde (0, 1/2 MIC, and MIC) for 30, 60, and 120 min.

성능/효과

After 7 days of storage, the green mold incidence of control fruits was 100% while that of panisaldehyde-treated (1 × MFC and 10 × MFC) fruits was only 90 ± 8.6% and 45 ± 5%, respectively.
In conclusion, our present study indicated that p-anisaldehyde exhibited strong antifungal activity against mycelia growths of P. digitatum and P. italicum. The inhibitory mechanism involved might have to do with the destruction of the cell wall integrity and membrane permeability
The results showed that the extracellular AKP activity in 1/2 MIC and MIC p-anisaldehyde-treated P. digitatum and P. italicum mycelia were all significantly (p < 0.05) higher than that of control (Fig. 4).

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