Cha, Joon Min
(Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University)
,
Park, Jong Eel
(Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University)
,
Choi, Sang Un
(Korea Research Institute of Chemical Technology)
,
Lee, Kang Ro
(Natural Products Laboratory, School of Pharmacy, Sungkyunkwan University)
Extensive column chromatography separation of the MeOH extract from the aerial parts of Phyllanthus urinaria afforded seventeen compounds (1 - 17). The structures of the compounds were elucidated by physicochemical and spectroscopic methods to be 5'-β-D-glucopyranosyloxyjasmonic butyl ester (...
Extensive column chromatography separation of the MeOH extract from the aerial parts of Phyllanthus urinaria afforded seventeen compounds (1 - 17). The structures of the compounds were elucidated by physicochemical and spectroscopic methods to be 5'-β-D-glucopyranosyloxyjasmonic butyl ester (1), (+)-cucurbic acid (2), dendranthemoside B (3), boscialin 4'-O-β-D-glucoside (4), 4,5-dihydroblumenol A (5), (6R,9R)-megastigman-4-ene-9,13-diol (6), (3S,5R,6S,9R)-3,6-dihydroxy-5,6-dihydro-β-ionol (7), (6S,9R)-roseoside (8), mallophenol B (9), icariside B5 (10), corchoinoside B (11), canangaionoside (12), 5,6-epoxy-3-hydroxy-7-megastigmen-9-one (13), icariside B2 (14), (7E)-2β,3β-dihydroxy-megastigm-7-en-9-one (15), betulalbuside A (16), and loliolide (17). The compounds 1, and 3 - 16 were isolated for the first time from this plant. The absolute stereochemistry of compound 1 was newly determined. The isolated compounds were tested for cytotoxic activity against four human tumor cell lines in vitro using a Sulforhodamin B bioassay, but all the compounds showed weak cytotoxic activities.
Extensive column chromatography separation of the MeOH extract from the aerial parts of Phyllanthus urinaria afforded seventeen compounds (1 - 17). The structures of the compounds were elucidated by physicochemical and spectroscopic methods to be 5'-β-D-glucopyranosyloxyjasmonic butyl ester (1), (+)-cucurbic acid (2), dendranthemoside B (3), boscialin 4'-O-β-D-glucoside (4), 4,5-dihydroblumenol A (5), (6R,9R)-megastigman-4-ene-9,13-diol (6), (3S,5R,6S,9R)-3,6-dihydroxy-5,6-dihydro-β-ionol (7), (6S,9R)-roseoside (8), mallophenol B (9), icariside B5 (10), corchoinoside B (11), canangaionoside (12), 5,6-epoxy-3-hydroxy-7-megastigmen-9-one (13), icariside B2 (14), (7E)-2β,3β-dihydroxy-megastigm-7-en-9-one (15), betulalbuside A (16), and loliolide (17). The compounds 1, and 3 - 16 were isolated for the first time from this plant. The absolute stereochemistry of compound 1 was newly determined. The isolated compounds were tested for cytotoxic activity against four human tumor cell lines in vitro using a Sulforhodamin B bioassay, but all the compounds showed weak cytotoxic activities.
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제안 방법
The cytotoxicities of compounds 1 - 17 against the A549 (a non small cell lung carcinoma), SK-OV-3 (ovary malignant ascites), SK-MEL-2 (skin melanoma), and HCT15 (colon adenocarcinoma) human cancer cell lines were evaluated using the SRB assay. But all the compounds showed little cytotoxicity against tested cell lines (IC50 >30 μM).
대상 데이터
). A voucher specimen (SKKU-NPL1405) of the plant was deposited at the School of Pharmacy at Sungkyunkwan University, Suwon, Korea.
성능/효과
In conclusion, two jasmonic acid derivatives (1 - 2), along with fifteen megastigmane derivatives (3 - 17) were isolated from the aerial parts of P. urinar. The absolute configuration of compound 1 was first determined.
참고문헌 (29)
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Xu, M.; Zha, Z. Z.; Qin, X. L.; Zhang, X. L.; Yang, C. R.; Zhang, Y. J. Chem. Biodivers. 2007, 4, 2246-2252.
Nguyen, V. T.: Phan, T. T. H.; Nguyen, H. N.; Nguyen, X. C.; Nguyen, P. T.; Bieke, D.; Andrea, G.; Yvan, V. H.; Joell, Q. L.; Chau, V. M. Phytochem. Lett. 2014, 7, 182-185.
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