[논문]DNA Barcoding of the Marine Proteced Species Pseudohelice subquadrata (Decapoda, Varunidae, Pseudohelice) from the Korean Waters

Kim, Ji Min; Kim, Jong-Gwan; Kim, So Yeon; Choi, Woo Yong; Kim, Hyung Seop; Kim, Min-Seop

doi:10.5635/ased.2020.36.3.036

DNA Barcoding of the Marine Proteced Species Pseudohelice subquadrata (Decapoda, Varunidae, Pseudohelice) from the Korean Waters 원문보기

Animal systematics, evolution and diversity : ASED, v.36 no.3, 2020년, pp.228 - 231

Kim, Ji Min (National Marine Biodiversity Institute of Korea) , Kim, Jong-Gwan (National Marine Biodiversity Institute of Korea) , Kim, So Yeon (National Marine Biodiversity Institute of Korea) , Choi, Woo Yong (National Marine Biodiversity Institute of Korea) , Kim, Hyung Seop (School of Marine Biotechnology, College of Marine Science, Kunsan National University) , Kim, Min-Seop (National Marine Biodiversity Institute of Korea)

Abstract ▼ AI-Helper

Pseudohelice subquadrata (Dana, 1851) is endangered due to its restricted habitat; hence, it has been designated as a marine protected species and endangered species by law in Korea. It has been recorded only Jeju-do and Geomun-do, Republic of Korea. The present study, is the first report on a cytochrome c oxidase subunit I DNA barcode for P. subquadrata. The maximum intra-specific genetic distance among all P. subquadrata individuals was found to be 0.5%, whereas inter-genetic distance within the same genus was 17.2-21.5% compared with Helice tientsinensis (Rathbun, 1931), H. tridens (De Haan, 1835), H. epicure (Ng et al., 2018), and Helicana wuana (Rathbun, 1931). Our barcoding data can thus be used as reference for restoration and conservation studies on P. subquadrata, which are designated as marine protected species.

주제어

표/그림 (2)

그림 Fig. 1. Pseudohelice subquadrata habitat (A), upper view (B).
표 Table 1. Pairwise genetic distances based on 658 bp size of cytochrome c oxidase subunit I (COI) genes from Pseudohelice sub-quadrata, Helice and Helicana species

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대상 데이터

Genomic DNA was extracted from ambulatory leg muscle tissue using the DNeasy Blood & Tissue Kit (Qiagen, Hilden, Germany). All genomic DNA samples were deposited at the National Marine Biodiversity Institute of Korea (MABIK) (Seocheon, Korea) (Voucher Nos. MABIK GR00002588-2591). A portion of the COI sequence was amplified by polymerase chain reaction (PCR) using the primers LCO1490 and HCO2198 (Folmer et al.

이론/모형

Pairwise genetic distances among sequences were calculated using the MEGA X program (Kumar et al., 2018) with the Kimura two-parameter model (Kimura, 1980). In order to demonstrate the utility of DNA barcode genes, COI sequences of Helice tientsinensis (Rathbun, 1931) (AB334547), H.

참고문헌 (17)

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