[논문]Distribution of Pectobacterium Species Isolated in South Korea and Comparison of Temperature Effects on Pathogenicity

Jee, Samnyu; Choi, Jang-Gyu; Lee, Young-Gyu; Kwon, Min; Hwang, Ingyu; Heu, Sunggi

doi:10.5423/ppj.oa.09.2019.0235

Distribution of Pectobacterium Species Isolated in South Korea and Comparison of Temperature Effects on Pathogenicity 원문보기

The plant pathology journal, v.36 no.4, 2020년, pp.346 - 354

Jee, Samnyu (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) , Choi, Jang-Gyu (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) , Lee, Young-Gyu (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) , Kwon, Min (Highland Agriculture Research Institute, National Institute of Crop Science, Rural Development Administration) , Hwang, Ingyu (Department of Agricultural Biotechnology, Seoul National University) , Heu, Sunggi (Crop Cultivation and Environment Research Division, National Institute of Crop Science, Rural Development Administration)

Abstract ▼ AI-Helper

Pectobacterium, which causes soft rot disease, is divided into 18 species based on the current classification. A total of 225 Pectobacterium strains were isolated from 10 main cultivation regions of potato (Solanum tuberosum), napa cabbage (Brassica rapa subsp. pekinensis), and radish (Raphanus sativus) in South Korea; 202 isolates (90%) were from potato, 18 from napa cabbage, and five from radish. Strains were identified using the Biolog test and phylogenetic analysis. The pathogenicity and swimming motility were tested at four different temperatures. Pectolytic activity and plant cell-wall degrading enzyme (PCWDE) activity were evaluated for six species (P. carotovorum subsp. carotovorum, Pcc; P. odoriferum, Pod; P. brasiliense, Pbr; P. versatile, Pve; P. polaris, Ppo; P. parmentieri, Ppa). Pod, Pcc, Pbr, and Pve were the most prevalent species. Although P. atrosepticum is a widespread pathogen in other countries, it was not found here. This is the first report of Ppo, Ppa, and Pve in South Korea. Pectobacterium species showed stronger activity at 28℃ and 32℃ than at 24℃, and showed weak activity at 37℃. Pectolytic activity decreased with increasing temperature. Activity of pectate lyase was not significantly affected by temperature. Activity of protease, cellulase, and polygalacturonase decreased with increasing temperature. The inability of isolated Pectobacterium to soften host tissues at 37℃ may be a consequence of decreased motility and PCWDE activity. These data suggest that future increases in temperature as a result of climate change may affect the population dynamics of Pectobacterium.

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표/그림 (8)

그림 Fig. 1. Incidence of soft rot on potato and napa cabbage in seven areas of Korea during 2016-2017.
표 Table 1. List of reference species and their GenBank accession numbers
그림 Fig. 2. A concatenated neighbor-joining phylogenetic analysis of 225 Pectobacterium strains based on 16S rRNA and recA gene sequences.
표 Table 2. Distribution of Pectobacterium species based on location and host in South Korea
그림 Fig. 3. The diameter of macerated tissue in potato and napa cabbage inoculated with Pectobacterium species.
그림 Fig. 4. Comparison of swimming motility of Pectobacterium species at four different temperatures.
그림 Fig. 5. Comparison of pectolytic activity with Pectobacterium species at 28°C and 37°C.
그림 Fig. 6. Comparison of plant cell-wall degrading enzyme activity of Pectobacterium species at 24°C, 28°C and 37°C.

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문제 정의

, 2018). The objective of this study was to identify the Pectobacterium species collected from South Korea based on the current taxonomy.

제안 방법

The pathogenicity was highest at 28°C and 32°C, and no pathogenicity was observed at 37°C. In order to investigate whether temperature affected the viability of the bacterium or its pectolytic activity as the key factor for soft rot pathogenicity, we assessed the swimming motility and pectolytic activity of the isolated bacteria. The swimming motility of Pod, Pcc, and Pbr was stable up to 32°C but markedly reduced at 37°C; however, the motility of Pve, Ppa, and Ppo reduced gradually with increasing temperature (Fig.
Comparison of pectolytic activity with Pectobacterium species at 28°C and 37°C. The assessment of pectolytic activity was performed with diameter of a cavity on crystal violet pectate (CVP) medium. Different letters indicate significant difference according to ANOVA with Duncan test at *P < 0.

대상 데이터

Field selection was based on the major area of cultivation for the three crops. For sample collection, infected plant tissues were randomly collected during the growing season from ten growing areas in South Korea: Hongcheon, Gangneung, Pyeongchang, Jeongseon, Yeongwol, Muju, Geochang, Miryang, Boseong, and Jeju. The infected plant tissues were first sterilized with 1% hypochlorite solution for 90 s, then rinsed in distilled water, and then macerated with 1 ml of distilled water.
A concatenated neighbor-joining phylogenetic analysis of 225 Pectobacterium strains based on 16S rRNA and recA gene sequences. This analysis was performed with 225 isolated strains and 48 reference strains. This collection was divided into 6 clades including P.
01. This test was performed with 7 Pectobacterium species including a reference strain of P. atrosepticum.
Pectobacterium spp. were collected from 10 of the main potato, napa cabbage, and radish cultivation areas in South Korea (Table 2, Fig. 1). Of the 225 identified strains, 174 isolates (77.

이론/모형

, 2007). Phylogenetic analysis was performed by the neighbor-joining method (Saitou and Nei, 1987). Twenty-four sequences of 13 reference strains were retrieved from NCBI (Table 1).

성능/효과

In total, four Ppa isolates were identified— one each from the samples collected in Hongcheon and Miryang, respectively, and two from the strains obtained from the MST. Given that the MST samples were collected during 1997-2000 and Ppa strains were identified in this collection, it can be assumed that Ppa isolates have existed in South Korea for at least 20 years. Eight known species of the soft rot disease complex were not present among the 225 isolated strains: Pa, P.
(2017) showed that Cel activity of Dickeya species was mainly regulated by temperature, but there was a larger effect of species on pectinase and Prt activities. In this study, Cel and Prt appeared to be strongly affected by high temperature, but Pel and Peh were only slightly affected by both species and temperature.
, 2018, 2019b, 2019c). Of the 18 species, P. fontis, P. aquaticum, P. polonicum, and Pve were isolated from aquatic ecosystems including, groundwater, waterways, waterfalls, and streams.
Our results showed that Pod, Pcc, Pbr, and Pve were the most prevalent Pectobacterium species isolated from potato and napa cabbage fields in South Korea. In addition to these four species, two isolates each of Ppa and Ppo were isolated.

후속연구

, 2004). The high prevalence of Pod may be caused by crop rotation of potato with napa cabbage in South Korea; further studies are required to verify this. The high potato cultivation temperature may limit the growth of P.

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