[국내논문]Prevalence and Characteristics of Antimicrobial-Resistant Staphylococcus aureus and Methicillin-Resistant Staphylococcus aureus from Retail Meat in Korea원문보기
Kim, Yong Hoon
(Food Microbiology Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
,
Kim, Han Sol
(Food Microbiology Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
,
Kim, Seokhwan
(Food Microbiology Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
,
Kim, Migyeong
(Food Microbiology Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
,
Kwak, Hyo Sun
(Food Microbiology Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety)
This study was to investigate the prevalence and characteristics of antimicrobial-resistant Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) from 4,264 retail meat samples including beef, pork, and chicken in Korea between 2013 and 2018. A broth microdilution antimicrobial susceptibi...
This study was to investigate the prevalence and characteristics of antimicrobial-resistant Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) from 4,264 retail meat samples including beef, pork, and chicken in Korea between 2013 and 2018. A broth microdilution antimicrobial susceptibility testing was performed for S. aureus. Molecular typing by multilocus sequence typing (MLST), spa typing, and pulsed-field gel electrophoresis (PFGE), was performed on mecA-positive S. aureus strain. S. aureus was isolated at a rate of 18.2% (777/4,264), of which MRSA comprised 0.7% (29 strains). MLST analysis showed that 11 out of the 29 MRSA isolates were predominantly sequence type (ST) 398 (37.9%). In addition, ST72, ST692, ST188, ST9, and ST630 were identified in the MRSA isolates. The spa typing results were classified into 11 types and showed a high correlation with MLST. The antimicrobial resistance assays revealed that MRSA showed 100% resistance to cefoxitin and penicillin. In addition, resistance to tetracycline (62.1%), clindamycin (55.2%), and erythromycin (55.2%) was relatively high; 27 of the 29 MRSA isolates exhibited multidrug resistance. PFGE analysis of the 18 strains excluding the 11 ST398 strains exhibited a maximum of 100% homology and a minimum of 64.0% homology. Among these, three pairs of isolates showed 100% homology in PFGE; these results were consistent with the MLST and spa typing results. Identification of MRSA at the final consumption stage has potential risks, suggesting that continuous monitoring of retail meat products is required.
This study was to investigate the prevalence and characteristics of antimicrobial-resistant Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) from 4,264 retail meat samples including beef, pork, and chicken in Korea between 2013 and 2018. A broth microdilution antimicrobial susceptibility testing was performed for S. aureus. Molecular typing by multilocus sequence typing (MLST), spa typing, and pulsed-field gel electrophoresis (PFGE), was performed on mecA-positive S. aureus strain. S. aureus was isolated at a rate of 18.2% (777/4,264), of which MRSA comprised 0.7% (29 strains). MLST analysis showed that 11 out of the 29 MRSA isolates were predominantly sequence type (ST) 398 (37.9%). In addition, ST72, ST692, ST188, ST9, and ST630 were identified in the MRSA isolates. The spa typing results were classified into 11 types and showed a high correlation with MLST. The antimicrobial resistance assays revealed that MRSA showed 100% resistance to cefoxitin and penicillin. In addition, resistance to tetracycline (62.1%), clindamycin (55.2%), and erythromycin (55.2%) was relatively high; 27 of the 29 MRSA isolates exhibited multidrug resistance. PFGE analysis of the 18 strains excluding the 11 ST398 strains exhibited a maximum of 100% homology and a minimum of 64.0% homology. Among these, three pairs of isolates showed 100% homology in PFGE; these results were consistent with the MLST and spa typing results. Identification of MRSA at the final consumption stage has potential risks, suggesting that continuous monitoring of retail meat products is required.
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문제 정의
aureus and MRSA from retail meats in Korea. Therefore, aim of this study is to investigate the prevalence of antimicrobial-resistant S. aureus and MRSA strains from retail meats (beef, pork, and chicken) in Korea and their molecular characteristics.
This study has a limitation regarding the confirmation of MRSA isolates. Because mecC-positive strains were not determined, the prevalence of MRSA in our study could be underestimated.
대상 데이터
aureus (n=777) were carried out using the minimum inhibitory concentration (MIC) method. For the MIC test, a custom-made EUST plate (Thermo Trek Diagnostics, OH, USA) was used. The inoculum of S.
1. Dendrogram of pulsed field gel electrophoresis (PFGE) with SmaI and characteristics of the 18 MRSA isolates. MRSA, methicillinresistant Staphylococcus aureus.
데이터처리
The statistical significance of differences between proportions was evaluated with Chi-square (χ2) tests using Epitools (Sergeant, 2018).
이론/모형
Antimicrobial susceptibility tests for S. aureus (n=777) were carried out using the minimum inhibitory concentration (MIC) method. For the MIC test, a custom-made EUST plate (Thermo Trek Diagnostics, OH, USA) was used.
The antibiotics used in the MIC test and the test ranges are given in Table 1. Susceptibility results in the form of MICs were interpreted by referring to the Clinical and Laboratory Standards Institute (CLSI) guidelines and European Committee on Antimicrobial Susceptibility Testing (EUCAST) guidelines (Table 1). For confirmation of MRSA, only strains showing resistance to cefoxitin were selected.
성능/효과
Among the 777 S. aureus strains, 64 cefoxitin-resistant strains were tested for the mecA gene, and 29 strains (0.7%) were identified as MRSA (Table 3). Year-wise, 6 strains were identified in 2013, 2 were identified in 2014, 5 were identified in 2015, 2 were identified in 2016, 9 were identified in 2017, and 5 were identified in 2018.
후속연구
The identification of MRSA in the final food consumption stage, which has been isolated at a low rate, is indicative of potential risks, and its presence or absence must be closely monitored at the national level. In addition, future studies may be directed towards monitoring the distribution and transmission of healthcare-associated (HA) MRSA, CA-MRSA, and LA-MRSA in farms, slaughterhouses, and retail foods.
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