[논문]Antioxidant activities of flower, berry and leaf of Panax ginseng C. A. Meyer

Ryu, Hee-Jeong; Jung, Chul-Jong; Beik, Gyung-Yun

doi:10.9721/kjfst.2020.52.4.342

Antioxidant activities of flower, berry and leaf of Panax ginseng C. A. Meyer 원문보기

한국식품과학회지 = Korean journal of food science and technology, v.52 no.4, 2020년, pp.342 - 349

Ryu, Hee-Jeong (Department of OkChundang Research institute) , Jung, Chul-Jong (Department of OkChundang Research institute) , Beik, Gyung-Yun (Department of OkChundang Research institute)

Abstract ▼ AI-Helper

This study was conducted to investigate the applicability of the ground parts such as flower (GF), berry (GR), and leaf (GL) from Panax ginseng C. A. Meyer. The ground parts were extracted from hot water (WE) and 60% ethanol (EE). Total polyphenol and flavonoid contents were 15.02-32.74 and 21.60-484.05 mg GAE/g, respectively. Hot water extract of ginseng leaf (GLWE) and 60% ethanol extract of ginseng leaf (GLEE) showed higher total polyphenol and total flavonoid contents than other extracts. Crude saponin contents were found in the range of 15.30-37.27%. Antioxidant activity of these extracts from ginseng was also analyzed by DPPH, ABTS, H2O2 scavenging activity, reducing power, and inhibition effect on lipid peroxidation. We confirmed the results that hot water extract of ginseng leaf (GLWE), 60% ethanol extract of ginseng leaf (GLEE) has high anti-oxidative effects. According to the antioxidant activity results of each extract of ginseng flower, ginseng berry, and ginseng leaf, it is judged that their availability is very high, and if proper processing is performed, it can be used as a functional raw material.

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표/그림 (6)

표 Table 1. Total polyphenol, flavonoid and crud saponin content of hot water and 60% ethanol extracts of flower, berry, and leaf from Panax ginseng C. A. Meyer
그림 Fig. 1. DPPH radical scavenging activity of hot water and 60% ethanol extracts of flower, berry, and leaf from Panax ginseng C. A. Meyer.
그림 Fig. 2. ABTS radical scavenging activity of hot water and 60% ethanol extracts of flower, berry, and leaf from Panax ginseng C. A. Meyer.
그림 Fig. 3. Reducing power of hot water and 60% ethanol extracts of flower, berry, and leaf from Panax ginseng C. A. Meyer.
그림 Fig. 4. H₂O₂ scavenging activity of hot water and 60% ethanol extracts of flower, berry, and leaf from Panax ginseng C. A. Meyer.
그림 Fig. 5. Inhibitory effects of hot water and 60% ethanol extracts of flower, berry, and leaf from Panax ginseng C. A. Meyer on linoleic acid peroxidation.

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문제 정의

The purpose of this study was to investigate the antioxidant activity, crude saponin content of the above-ground parts of ginseng to find various ways to utilize ginseng flower, berry, and leaf and to provide basic data for the development of health functional food products.

제안 방법

Extraction and determination of crude saponin were carried out by modifying the method of Korean food standards codex. The samples (5 g) were placed into the reflux extractor and extracted with 50 mL water-saturated butanol at 70°C for 1 h.
The hot water extracts were expressed as GFWE, GRWE, GLWE (hot water extract of ginseng flower; GFWE, hot water extract of ginseng berry; GRWE and hot water extract of ginseng leaf; GLWE). And the ethanol extracts were extracted with 60% ethanol and expressed as GFEE, GREE, GLEE (60% ethanol extract of ginseng flower; GFEE, 60% ethanol extract of ginseng berry; GREE and 60% ethanol extract of ginseng leaf; GLEE).

대상 데이터

Folin-Ciocalteu reagent, DPPH (1,1-diphenyl-2-picrylhydrazyl), ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), Hydrogen peroxide (H2O2), gallic acid, BHA (butylated hydroxyanisole), ascorbic acid was purchased from Sigma-Aldrich Co. (St, Louis, MO, USA).
The flower, berry, and leaf of ginseng used in this study were purchased from Yeongcheon branch of Okchundang (Okchundang Co., Daegu, Korea). The dried flower, berry, and leaf of 4-year-old ginseng were extract ed 80o C for more than 8 h (three times) in the water of decuple (sample: sol vent extraction=1:10, w/v) of the sample using a reflux extraction for the physiologically activities.

데이터처리

Each value is mean±SE (n≥3) and different superscript letters (a-g) are significantly different at p<0.05 by Duncan’s multiple range test.
Each value is mean±SE (n≥3) and different superscript letters (a-j) are significantly different at p<0.05 by Duncan’s multiple range test.
Each value is mean±SE (n≥3) and different superscript letters (a-p) are significantly different at p<0.05 by Duncan’s multiple range test.
Each value is mean±SE (n≥3) and different superscript letters (a-q) are significantly different at p<0.05 by Duncan’s multiple range test.
Statistical significance of differences between groups was analyzed using a one-way ANOVA analysis of variance with Duncan’s multiple tests (SPSS, version 17.0, Chicago, IL, USA).

이론/모형

The DPPH radical scavenging capacity of samples was determined based on the method described by Blois (1958). A total of 1 mL of ethanolic DPPH radical (0.
The lipid peroxidation inhibitory activity of the lipids was measured in a thiobarbituric acid assay according to the method of Nakatan and Kikuzaki (1987). A mixture consisting of 5 mg/mL samples, 1 mL of 50 mM sodium phosphate buffer (pH 7.
Total phenolic contents were determined using a FolinCiocalteau reagent assay (Dewanto et al., 2002). Each extract was dissolved in methanol (0.

성능/효과

98%) and the highest DPPH scavenging activity was observed in the GLWE. Also, as shown in Fig. 1., GFWE (69.20%) and GRWE (64.86%) showed high scavenging ability at 5 mg/mL concentration of each extracts also, GFWE and GRWE activities were higher than those at higher concentrations. These results have shown that the effective concentration of the different scavenging capacities was 5 mg/mL depending on the amount of each extract.
42 mg GAE/g. As a result, it was confirmed that the hot water and ethanol extracts from ginseng leaf has the highest total flavonoid contents similar to the trend of total polyphenol contents. The ground parts of ginseng with a high physiologically actives substance such as total polyphenols and flavonoids show the possibility to be used as a material for health supplements.
5 to 5 mg/mL with increasing concentration. At the 5 mg/mL, GLWE 89.20%, GRWE 88.21% and GFWE 85.68% shown the highest inhibition activity and these extracts were significantly different in the order of GLWE (86.33%), GLEE (73.91%), GRWE (67.13%), GFWE (67.13%) at 10 mg/ mL. The RC50 value of ABTS radical scavenging activity with 60% ethanol and hot water extract were GLWE (0.
At the concentration of 1 mg/mL, these extracts showed activity in order of GLWE (64.67%) > GLEE (47.46%) > GRWE (30.98%) and the highest DPPH scavenging activity was observed in the GLWE.
When the absorbance value of the negative control on the 4 days is taken as 100%. In the positive control 1% butylated hydroxytoluene (BHT), oxidation was suppressed to 93.02%, GREE 55.81%, GFEE 41.86%, GLEE 38.37%, GRWE 32.56%, GFWE 23.26% and GLWE 9.30%. Among them, ethanol extracts from flower, berry, and leaf from ginseng showed an excellent inhibitory effect on lipid peroxidation.
, 2015). It was confirmed that the ground parts had a significantly higher total phenol content than the roots, these results are similar to those of ginseng and contain higher polyphenols than root extracts.
The DPPH scavenging activity range of samples was GFWE (9.96-69.20%), GFEE (0.36- 62.68%), GRWE (16.30-64.86%), GREE (8.88-63.22%), GLWE (−47.64-64.67%) and GLEE (15.22-61.78%), respectively.
The total flavonoids showed 330.29±13.83 mg GAE/g in GLWE and 484.05±5.56 mg GAE/g in GLEE.
The total polyphenol contents of GFWE and GFEE were 17.17±0.05, 15.02±0.05 mg GAE/g, respectively.

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