[논문]참나무시들음병 신갈나무 피해목과 건전목의 해부학적 특징 비교

JEON, Woo-Seok; LEE, Hyun-Mi; PARK, Ji-Hyun

doi:10.5658/wood.2020.48.6.807

참나무시들음병 신갈나무 피해목과 건전목의 해부학적 특징 비교
Comparison of Anatomical Characteristics for Wood Damaged by Oak Wilt and Sound Wood from Quercus mongolica 원문보기

목재공학 = Journal of the Korean wood science and technology, v.48 no.6, 2020년, pp.807 - 819

JEON, Woo-Seok (Division of Timber Engineering, National Institute of Forest Science) , LEE, Hyun-Mi (Division of Timber Engineering, National Institute of Forest Science) , PARK, Ji-Hyun (Division of Forest Insect Pests and Diseases, National Institute of Forest Science)

초록
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본 연구에서는 참나무시들음병에 감염된 신갈나무의 기초자료를 확보하기 위하여, 해부학적 특징을 비교·분석하고자 하였다. 해부학적 특징을 비교하기 위해, 감염목, 고사목 및 건전재 신갈나무를 수에서부터 10~50번째 연륜까지 10연륜 단위로 광학현미경과 주사전자현미경을 이용하여 관찰하였다. 목섬유 길이는 수에서부터 5연륜 단위로 측정하였고, 도관요소 직경 및 타일로시스를 포함한 도관의 비율은 10연륜별로 측정하였다. 횡단면 관찰 결과, 감염목과 고사목에서는 도관 내강에서 티일로시스와 함께 균사체가 관찰되었다. 목섬유 길이 및 도관요소 직경은 조사목 간 큰 차이를 보이지 않았다. 목섬유 길이는 미숙재 부위인 20~30연륜 이후 안정화되는 경향을 보였다. 타일로시스를 포함한 도관의 평균 비율은 감염목이 가장 높았고 특히, 감염목과 고사목은 40~50연륜에서 타일로시스의 비율이 가장 높았다. 이를 통하여, 참나무시들음병 피해목과 건전재 간에 큰 차이는 타일로시스의 비율로 생각되며, 피해목 이용에 있어 기초자료로 활용 가능할 것이라 사료된다.

Abstract ▼ AI-Helper

The aim of this study was to investigate the anatomical characteristics of Quercus mongolica infested by oak wilt disease. To analysis the anatomical characteristics of the wood specimens infested by the oak wilt, the anatomical structures of an infected wood, a dead wood, and sound wood were observed at 10-year-old intervals from 10 to 50 annual rings using both an optical and a scanning electron microscope. The fiber length was measured in units of 5 annual rings from the pith, and the diameter of the vessel element and the ratio of the vessel including the tyloses were measured for each 10 annual ring. In the cross section, on the infected and dead wood specimens, mycelium was also observed with the tyloses in the vessel. There was no signification difference between the wood specimens in the fiber length and the vessel diameter of the vessel element. The fiber length was not difference after 20-30 annual rings which is a part of juvenile wood. The average of the vessel ratio including tyloses in the infected wood was the highest. Especially, the ratio of tyloses was the highest 40-50 annual rings in the infected wood and the dead wood. Therefore, the large difference between the infested wood by oak wilt and the sound wood was the ratio of tyloses. This result can be used as a basic data to utilize the infested wood.

주제어

표/그림 (8)

그림 Fig. 1. Disk from infested and dead trees by oak wilt disease, and from a sound tree.
표 Table 1. Basic information of infested and dead woods by oak wilt disease, and from a sound wood
그림 Fig. 2. Optical micrographs on the cross sections of infested, dead, and sound woods by annual rings. Arrow : tylosis in vessel
그림 Fig. 3. SEM images on cross section of infested, dead, and sound woods by annual rings. 30 : 30th tree ring from the pith, 50 : 50th tree ring from the pith Red circle : tylosis bud, White arrow : mycelium
그림 Fig. 4. Fiber length of infested, dead, and sound woods by annual rings. (A) : Earlywood, (B) Latewood
표 Table 2. Fiber length and vessel dimension of infested, dead, and sound woods
그림 Fig. 5. Vessel element diameter of infested, dead, and sound woods by annual rings. (ET) : Earlywood – Tangential direction, (ER) : Earlywood – Radial direction (LT) : Latewood - Tangential direction, (LR) : Latewood – Radial direction
그림 Fig. 6. Vessel element diameter of infested, dead, and sound woods by annual rings. IW : Infested wood, DW : Dead wood, SW : Sound wood

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제안 방법

The tangential and radial diameters were measured by categorizing earlywood and latewood using the annual rings on a cross section at 50x magnification using the prepared permanent slides. The ratio of vessels with tylosis was calculating by estimating the number of vessels with tylosis after measuring the number of 30 vessels from each age group by dividing the wood at 10 ring intervals from the pith to the bark.
6), respectively. The results of this experiment showed trends that were consistent with optical microscope results.
Although there are studies on trees infected with oak wilt disease, basic anatomical data that can facilitate the utilization of the damaged tree is insufficient. Therefore, in this study, the anatomical characteristics of damaged Q.mongolica (infected and dead) trees were compared with those of sound trees and analyzed to obtain basic data for the utilization of trees damaged by oak wilt disease.
This study compared and analyzed the anatomical characteristics of the damaged and dead wood affected by oak wilt disease and those of sound wood in Korea. Tylosis was frequently observed in the cross sections of infected and dead woods along with mycelium, indicating the degree of infection in the 30^th–50^th annual rings.

이론/모형

They were washed with distilled water, dyed with methylene blue. The length of wood fibers of 30 samples of each type of wood were measured using the the DeltaPix (version 5.3.11) program, which were chosen from the annual rings of both earlywood and latewood.

성능/효과

The radial diameters of the vesselelement in earlywood and latewood was larger than the tangential diameters. The tangential diameter of vessel elements for each annual ring of earlywood, latewood, and radial diameters was the smallest value in 10 annual rings of all of the infected, dead, and sound wood, and the diameter tended to increase as the number of rings increased (Fig. 5). Eom (2015) reported that the average diameter of the tangential line of the vessel element of domestic Q.
Therefore, there was a confirmed difference in the ratio of tylosis in the areas of juvenile and mature wood in the oak wilt-damaged wood and sound wood of Q. mongolica. It is considered that this difference in the tylosis ratio blocks water movement, causing the infection and death of the oak wilt diseased tree.
To observe the anatomical characteristics of the trees affected by oak wilt disease, two Q. mongolica trees (one infected and one dead) were selected, which were confirmed to be damaged by oak wilt disease among the oak trees that grow wild in Dongtan-dong, Hwaseong-si. To compare the anatomical characteristics of infected and dead oak trees with those of sound trees, one sound tree was selected from Chuncheon-si, Gangwon-do.

참고문헌 (24)

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상세보기
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원문보기 상세보기
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