[논문]방사선 돌연변이 육종기술을 통한 고 아미노산 함유 김(Pyropia yezoensis) 돌연변이 개발

이학증

doi:10.5657/kfas.2021.0982

[국내논문] 방사선 돌연변이 육종기술을 통한 고 아미노산 함유 김(Pyropia yezoensis) 돌연변이 개발
Development of Pyropia yezoensis Mutant with Improved Amino Acid Content Using Gamma Rays 원문보기

한국수산과학회지 = Korean journal of fisheries and aquatic sciences, v.54 no.6, 2021년, pp.982 - 988

이학증 (국립수산과학원 수산종자육종연구소)

Abstract ▼ AI-Helper

Gamma irradiation is one of the simple methods used to induce mutagenesis. Therefore, it is widely used for the development of breeding lineages of plants and algae. In this study, it was developed a new variety of Pyropia yezoensis using gamma irradiation. It was applied a dose of 1 kGy and named the developed mutant Py1k. The blade with width of the mutant was narrower and the blade length was longer than those of the wild type. To further investigate the mutant, it was analyzed the nutrient composition and antioxidant activity. In comparison to those in the wild type, it was found a higher amino acid composition and marginally increased antioxidant activity in Py1k. Based on these results, it was suggests that our protocol can be utilized to develop Pyropia species with improved nutritional quality through gamma irradiation.

주제어

표/그림 (7)

그림 Fig. 1. Comparison of morphological change between Pyropia yezoensis WT and mutant Py1k during 6 weeks of laboratory culture.
그림 Fig. 2. Growth rate of WT and Py1k mutant gametophytic blades cultured of 10°C for 1 to 6 weeks. WT, wild type. Average±standard deviation, n=10.
표 Table 1. Total amino acid composition of Pyropia yezoensis WT and mutant Py1k.
표 Table 2. Free amino acid composition of Pyropia yezoensis WT and mutant Py1k.
그림 Fig. 3. Measurement of DPPH radical scavenging activity in Pyropia yezoensis WT and mutant Py1k. DPPH, 1,1-diphenyl-2-picrylhydrazyl; WT, wild type. Average±standard deviation, n=3.
그림 Fig. 4. Measurement of FRAP assay in Pyropia yezoensis WT and mutant Py1k. FRAP, ferric-reducing antioxidant potential; WT, wild type. Average±standard deviation, n=3.
그림 Fig. 5. Measurement of total phenolic content in Pyropia yezoensis WT and mutant Py1k. WT, wild type. Average±standard deviation, n=3.

AI 본문요약
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문제 정의

이에, 본 연구에서는 방사선 조사를 통하여 방사무늬김의 새로운 품종을 개발하고자 하였으며 대조군과 선별된 신품종에 대한 영양성분과 항산화 활성 조사를 수행하였다. 또한 앞으로의 김 돌연변이 육종방법에 대한 기초자료로서 제공하고자 한다.
이에, 본 연구에서는 방사선 조사를 통하여 방사무늬김의 새로운 품종을 개발하고자 하였으며 대조군과 선별된 신품종에 대한 영양성분과 항산화 활성 조사를 수행하였다. 또한 앞으로의 김 돌연변이 육종방법에 대한 기초자료로서 제공하고자 한다.

제안 방법

본 연구에서는 방사선을 이용한 육종 방법을 통해 대조군과 형태가 다르게 성장하고 맛과 풍미에 관련된 아미노산과 항산 화 활성이 향상된 품종을 선별하였다. 이러한 결과는 새로운 김 품종을 개발 육종하는 방법으로서 활용이 가능할 것으로 판단 하며 향후 분자생물학적 연구를 통해 분석이 이루어져야 될 것 으로 사료된다.

데이터처리

본 실험의 통계처리는 SPSS Statistics Ver. 20 (IBM, USA)를 사용하였으며, student t-test법으로 대조군과 선별된 돌연변이 품종간의 차이를 분석하였다.

후속연구

본 연구에서는 방사선을 이용한 육종 방법을 통해 대조군과 형태가 다르게 성장하고 맛과 풍미에 관련된 아미노산과 항산 화 활성이 향상된 품종을 선별하였다. 이러한 결과는 새로운 김 품종을 개발 육종하는 방법으로서 활용이 가능할 것으로 판단 하며 향후 분자생물학적 연구를 통해 분석이 이루어져야 될 것 으로 사료된다.

참고문헌 (48)

Baek SY, Kim SJ, Kim DH and Kim MR. 2019. Comparison of quality characteristics and antioxidant activities between Porphyra yezoensis and Porphyra dentata in Korea. Korean J Soc Food Sci Nutr 48, 1233-1243. https://doi.org/10.3746/jkfn.2019.48.11.1233.

상세보기
Benzie IFF and Strain JJ. 1996. The ferric reducing ability of palsma (FRAP) as a measure of "Antioxidant power": The FRAP assay. Anal Biochem 239, 70-79. https://doi.org/10.1006/abio.1996.0292.

상세보기
Cho SH, Yoo SC, Zhang H, Pandeya D, Koh HJ, Hwang JY, Kim GT and Paek NC. 2013. The rice narrow leaf2 and narrow leaf3 loci encode WUSCHEL-related homeobox 3A (OsWOX3A) and function in leaf, spikelet, tiller and lateral root development. New Phytol 198, 1071-1084. http://doi.org/10.1111/nph.12231.

상세보기
Choi JI. 2020. Amino acid composition and antioxidative activities of mutant Pyropia yezoensis. Korean J Fish Aquat Sci 53, 524-529. https://doi.org/10.5657/KFAS.2020.0524.

원문보기 상세보기
Dawczynski C, Schubert R and Jahresis G. 2007. Amino acids, fatty acids, and dietary fibre in edible seaweed products. Food Chem 103, 891-899. https://doi.org/10.1016/j.foodchem.2006.09.041.

상세보기
Dewanto V, Xianzhong W and Liu RH. 2002. Processed sweet corn has higher antioxidant activity. J Agric Food Chem 50, 4959-4964. https://doi.org/10.1021/jf0255937.

상세보기
Ding H, Zhang B and Yan X. 2016. Isolation and characterization of a heat-resistant strain with high yield of Pyropia yezoensis Ueda (Bangiales, Rhodophyta). Aquac Fish 1, 24-33. https://doi.org/10.1016/j.aaf.2016.09.001.

상세보기
Feurtado JA, Huang D, Wicki-Stordeur L, Hemstock LE and Potentier MS. 2011. The Arabidopsis C2H2 zinc finger Indeterminate Domain1/Enhydrous promotes the transition to germination by regulating light and hormonal signaling during seed maturation. Plant Cell 23, 1772-1794. https://doi.org/10.1105/tpc.111.085134.

상세보기
Furst P and Stehle P. 2004. What are the essential elements needed for the determination of amino acid requirements in humans?. J Nutr 134, 1558S-1565S. https://doi.org/10.1093/jn/134.6.1558S.

상세보기
Giri J, Vij S, Dansana PK and Tyagi AK. 2011. Rice A20/AN1 zinc finger containing stress-associated proteins (SAP1/11) and a receptor-like cytoplasmic kinase (OsRLCK253) interact via A20 zinc-finger and confer abiotic stress tolerance in transgenic Arabidopsis plants. New Phytol 191, 721-732. https://doi.org/10.1111/j.1469-8137.2011.03740.x.

상세보기
Hwang EK, Yotsukura N, Pang SJ, Su L and Shan TF. 2019. Seaweed breeding programs and progress in eastern Asian countries. Phycologia 58, 484-495. https://doi.org/10.1080/00318884.2019.1639436.

상세보기
Hwang MS, Kim SO, Lee YS, Park EJ, Kim SC, Ha DS, Gong YG, Baek JM and Choi HG. 2010. Isolation and characterization of pure lines of pigmentation and morphological mutants in Porphyra tenera Kjellman (Bangiales, Rhodophyta). Korean J Fish Aquat Sci 43, 495-502. https://doi.org/10.5657/kfas.2010.43.5.495.

원문보기 상세보기
Inoue N, Yamano N, Sakata K, Nagao, Hama Y and Yanagita T. 2009. The sulfated polysaccharide porphyran reduces apolipoprotein B100 secretion and lipid synthesis in HepG2 cell. Biosci Biotechnol Biochem 73, 447-449. https://doi.org/10.1271/bbb.80688.

상세보기
Jiang H, Zou D, Lou W, Deng Y and Zeng X. 2018. Effects of seawater acidification and alkalization on the farmed seaweed, Pyropia haitanensis (Bangiales, Rhodophyta), grown under different irradiance conditions. Algal Res 31, 413-420. https://doi.org/10.1016/j.algal.2018.02.033.

상세보기
Jimenez-Escrig A and Goni Cambrodon I. 1999. Nutritional evaluation and physiological effects of edinle seaweeds. Arch Latinoam Nutr 49, 114-120.
Jung SM, Kang SG, Kim KT, Lee HJ, Kim AR and Shin HW. 2015. The analysis of proximate composition, minerals and amino acid content of a red alga Pyropia dentata by cultivation sites1a. Korean J Environ Ecol 29, 1-6. https://doi.org/10.13047/KJEE.2015.29.1.001.

원문보기 상세보기
Jung HJ, Kim DH, Jeong MH, Lim CW, Shim KB and Cho YJ. 2017. Mineral analysis and nutritional evaluation according to production area of laver Porphyra tenera, Japanese kelp Saccharina japonicus, sea mustard Undaria pinnatifida and hijiki Sargassum fusiforme in Korea. J Fish Mar Sci Educ 29, 1624-1632. http://dx.doi.org/10.13000/JFMSE.2017.29.5.1624.

상세보기
Kim H and Lee SW. 2007. The effects of quercetin on paraquat induced cell damage. Korean J Soc Emerg Med 18, 41-48.
Kim KW, Hwang JH, Oh MJ,Kim MY, Choi MR and Park WM. 2014. Studies on the major nutritional components of commercial dried lavers Porphyra yezoensis cultivated in Korea. Korean J Food Preserv 21, 702-709. https://doi.org/10.11002/kjfp.2014.21.5.702.

원문보기 상세보기
KMI (Korea Marine Institute, Ministry of Oceans and Fisheries). 2020. Fisheries statistics. Retrieved from https://kfishinfo.co.kr on Jul 14, 2021.
Kwak YH, Bai SC and Kim DJ. 2010. Estimated availability and major minerals (Ca, P and Mg) contents bound neutral detergent fiber (NDF) of seaweeds. Korean J Soc Food Sci Nutr 39, 1073-1077. https://doi.org/10.3746/jkfn.2010.39.7.1073.

원문보기 상세보기
Kwon MJ and Nam TJ. 2006. Porphyra induces apoptosis related signal pathway in AGS gastric cancer cell lines. Life Sci 79, 1956-1962. https://doi.org/10.1016/j.lfs.2006.06.031.

상세보기
Lee HJ, Choi JI and Choi SJ. 2012. Physiological activities and amino acid compositions of Korean dried laver Porphyra Products. Korean J Fish Aquat Sci 45, 409-413. https://doi.org/10.5657/KFAS.2012.0409.

원문보기 상세보기
Lee HJ, Park EJ and Choi JI. 2019. Isolation, morphological characteristics and proteomic profile analysis of thermos-tolerant Pyropia yezoensis mutant in response to high-temperature stress. Ocean Sci J 54, 65-78. https://doi.org/10.1007/s12601-018-0060-9.

상세보기
Lee KH, Song SH and Jeong IH. 1987. Quality changes of dried lavers during processing and storage. 1. Quality evaluation of different grades of dried lavers and its changes during storage. J Kor Fish Soc 20, 408-418.
Li YX, Wang GC, Xu P, Fan XL, Niu JF and Zhou BC. 2008. Induction and characterization of green pigmentation mutant in Porphyra yezoensis Ueda. Aquaculture 282, 117-123. https://doi.org/10.1016/j.aquaculture.2008.05.026.

상세보기
Lim SN, Cheung PCK, Ooi VEC and Ang PO. 2002. Evaluation of antioxidative activity of extracts from a brown seaweed, Sargassum siliquastrum. J Agric Food Chem 50, 3862-3866. https://doi.org/10.1021/jf020096b.

상세보기
Ma Y. 2019. Characterization of a high-growth-rate mutant strain of Pyropia yezoensis using physiology measurement and transcriptome analysis. J Phycol 55, 651-662. https://doi.org/10.1111/jpy.12842.

상세보기
Mouritsen OG, Duelund L, Petersen MA, Hartmann AL and Frost MB. 2019. Umami taste, free amino acid composition, and volatile compounds of brown seaweeds. J Appl Phycol 31, 1213-1232. https://doi.org/10.1007/s10811-018-1632-x.

상세보기
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T and Okada K. 2012. Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis. Plant Cell 24, 519-535. https://doi.org/10.1105/tpc.111.092858.

상세보기
Nardmann J, Ji J, Werr W and Scanlon MJ. 2004. The maize duplicate genes narrow sheath1 and narrow sheath2 encode a conserved homeobox gene function in a lateral domain of shoot apical meristems. Development 131, 2827-2839. https://doi.org/10.1242/dev.01164.

상세보기
Niwa K, Hayashi Y, Abe T and Aruga Y. 2009. Induction and isolation of pigmentation mutants of Porphyra yezoensis (Bangiales, Rhodophyta) by heavy-ion beam irradiation. Phycol Res 57, 194-202. https://doi.org/10.1111/j.1440-1835.2009.00539.x.

상세보기
Niwa K, Yamamoto T, Furuita H and Abe T. 2011. Mutation breeding in the marine crop Porphyra yezoensis (Bangiales, Rhodophyta): cultivation experiment of the artificial red mutant isolated by heavy-ion beam mutagenesis. Aquaculture 314, 182-187. https://doi.org/10.1016/j.aquaculture.2011.02.007.

상세보기
Park CK, Park CH and Park JN. 2001. Extractive nitrogenous constituents of dried laver Porphyra yezoensis. Korean J Fish Soc 34, 394-402.
Park EJ and Choi JI. 2018. Resistance and proteomic response of microalgae to ionizing irradiation. Biotechnol Biopr Eng 23, 704-709. http://doi.org/10.1007/s12257-018-0468-1.

상세보기
Park SJ and Choi JI. 2020. De novo transcriptome analysis of high growth rate Pyropia yezoensis (Bangiales, Rhodophyta) mutant with high utilization nitrogen. Acta Bot Croat 79, 201-211. http://doi.org/10.37427/botcro-2020-026.

상세보기
Park WM, Kang DS and Bae TJ. 2014. Studies on organic acid, vitamin and free sugar contents of commercial dried lavers Porphyra yezoensis cultivated in Korea. J Korean Soc Food Sci Nutr 43, 172-177. https://doi.org/10.3746/jkfn.2014.43.1.172.

원문보기 상세보기
Ruiz-Capillas C and Moral A. 2001. Changes in free amino acids during chilled storage of hake (Merluccius merluccius, L.) in controlled atmospheres and their use as a quality control index. Eur Food Res Technol 212, 302-307. http://doi.org/10.1007/s002170000232.

상세보기
Shin DM, An SR, In SK and Koo JG. 2013. Seasonal variation in the dietary fiber, amino acid and fatty acid contents of Porphyra yezoensis. Korean J Fish Aquat Sci 46, 337-342. https://doi.org/10.5657/KFAS.2013.0337.

원문보기 상세보기
Shin YJ, Min SK, Kang YD, Lim JM, Park EJ, Hwang MS, Choi DW, Ahn JW, Park YI and Jung WJ. 2019. Characterization of high temperature-tolerant strains of Pyropia yezoensis. Plant Biotechnol Rep 12, 365-373. https://doi.org/10.1007/s11816-018-0499-2.

상세보기
Tsuge K, Kabe M, Yoshimura T, Sumi T, Tachibana H and Yamada K. 2004. Dietary effects of porphyran from Porphyra yezoensis in growth and lipid metabolism of Spraguedawley rats. Food Sci Technol Res 10, 147-151. https://doi.org/10.3136/fstr.10.147.

상세보기
Van Loo, EJ, Hoefkens C and Verbeke W. 2017. Healthy, sustainable and plant-based eating: Perceived (mis) match and involvement-based consumer segments as targets for future policy. Food Policy 69, 46-57. https://doi.org/10.1016/j.foodpol.2017.03.001.

상세보기
Vandenbussche M, Horstman A, Zethof J, Koes R, Rijpkema AS and Gerats T. 2009. Differential recruitment of WOX transcription factors for lateral development and organ fusion in Petunia and Arabidopsis. Plant Cell 21, 2269-2283. https://doi.org/10.1105/tpc.109.065862.

상세보기
Watanabe K and Sato Y. 1974. Meat flavor. Japan J Zootech Sci 45, 113-128.
Yan XH, Fujita Y and Aruga Y. 2000. Induction and characterization of pigmentation mutants in Porphyra yezoensis (Bangiales, Rhodophyta). J Appl Phycol 12, 69-81.
Yoshie Y, Suzuki T, Shirai T, Hirano T and Lee EH. 1993. Dietary fiber, minerals, free amino acids and fatty acid compositions in dried Nori of several culture places in Korea. J Tokyo Univ Fish 80, 197-203.
Zhao F, Du F, Oliveri H, Zhou L, Ali O, Chen W, Feng S, Wang Q, Lu S, Long M, Schneider K, Sampathkumar A, Godin C, Traas J and Jino Y. 2020. Microtubule-mediated wall anisotropy contributes to leaf blade flattening. Curr Biol 30, 3972-2985. https://doi.org/10.1016/j.cub.2020.07.076.

상세보기
Zhang Z, Runions A, Mentink RA, Kierzkowski D, Karady M, Hashemi B, Huijser P, Strauss S, Gan X, Ljung K and Tsiantis M. 2020. A WOX/auxin biosynthesis module controls growth to shape leaf form. Curr Biol 30, 4857-4868. https://doi.org/10.1016/j.cub.2020.09.037.

상세보기

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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