[논문]프로바이오틱스 유산균에 의한 질소 산화물 전환 및 철 이온 환원활성

김세림; 홍정일

doi:10.9721/kjfst.2021.53.6.785

프로바이오틱스 유산균에 의한 질소 산화물 전환 및 철 이온 환원활성
Bioconversion of nitrogen oxides and reduction of ferric ions by probiotic lactic acid bacteria 원문보기

한국식품과학회지 = Korean journal of food science and technology, v.53 no.6, 2021년, pp.785 - 791

김세림 (서울여자대학교 자연과학대학 식품응용시스템학부) , 홍정일 (서울여자대학교 자연과학대학 식품응용시스템학부)

초록
AI-Helper

다양한 유산균들이 생체에 유익한 영향을 주는 프로바이오틱스로 제시되고 있는 가운데, 본 연구에서는 L. kimchicus, L. lactis, L. casei, L. plantarum, LGG 및 Leu. mesenteroides 등 유산균 6종을 선별하여 균주별 nitrate의 환원과 nitrite의 소거, 그리고 철 이온에 대한 환원활성을 분석하였다. 사용된 6종의 유산균 중, 5종의 균주에서 72시간 배양시간 동안 nitrate에 대한 환원현상이 나타나지 않았으나, L. kimchicus는 nitrate의 환원을 통해 과량의 nitrite를 생성했다. 한편, nitrite 소거활성은 균주별로 상당한 차이를 보였으며, 특히 L. kimchicus는 nitrate와의 반응과 마찬가지로 가장 높은 소거능을 나타내어 반응시간과 균수에 따른 급격한 배지 중 nitrite 수준 감소를 유도하였다. Ferric ion의 ferrous ion으로의 환원에 있어서도 L. kimchicus가 다른 5종의 균주에 비해 420-1500배의 높은 활성을 보였다. L. kimchicus의 환원활성은 멸균처리 균주나 균체 용해물에서는 거의 발현되지 않아 생균의 대사능력에 의한 것으로 나타났다. 이러한 L. kimchicus의 활성은 프로바이오틱스로서의 효과뿐만 아니라, 관련 환원활성에 의한 NO 등 유용 포스트바이오틱스 대사물에 의한 효과도 기대할 수 있을 것으로 판단된다.

Abstract ▼ AI-Helper

Many lactic acid bacteria (LAB) have probiotic properties that exert various health benefits. In this study, the reduction potential of nitrogen oxide compounds and ferric ions by six LAB, including Lactobacillus kimchicus, L. lactis, L. casei, L. plantarum, L. rhamnosus GG, and Leuconostoc mesenteroides were evaluated. The L. kimchicus strain produced a substantial amount of nitrite reduced from nitrate added to the media, whereas the other five LAB strains did not. L. kimchicus also showed the most potent reducing activity of ferric to ferrous ions. However, the reduction potential of the autoclaved L. kimchicus was little pronounced. The scavenging activities of viable LAB or their cell lysates against different radicals were not consistent with the potency of the LAB's reducing ability. The present results indicate that L. kimchicus has a strong reduction potential for nitrogen oxides in viable status, and that this ability can be used as a probiotic property for various health benefits.

주제어

표/그림 (5)

그림 Fig. 1. Nitrate reducing activities of different LAB. Different numbers of LAB were grown in 5% (v/v) MRS broth containing 2 mM NaNO₃, and nitrite levels formed in culture broth were analyzed at 72 h (A). Time-dependent production of nitrite by L. kimchicus was measured during 72 h (B). Nitrite levels produced by viable and autoclaved L. kimchicus were also compared at 24 h (C). Each value represents the mean±SD (n=3). Different letters indicate a significant difference (p<0.05) based on one way ANOVA and the Tukey's HSD test. ** indicates a significant difference (p<0.01) based on Student's t-test (in C). N.D.; Not Detected
그림 Fig. 2. Nitrite scavenging activities of different LAB. Each bacterium was grown in 5% (v/v) MRS containing 50 μM NaNO₂, and changes in nitrite levels were analyzed at 24 h (A). The growth period-dependent changes of nitrite level by different LAB were analyzed during 72 h (B). Nitrite scavenging activities of autoclaved LAB were compared after 4 and 24 h incubation (C). Each value represents the mean±SD (n=3). Different letters indicate a significant difference (p<0.05) based on one way ANOVA and the Tukey's HSD test.
그림 Fig. 3. Reducing abilities on ferric to ferrous ion by different LAB. Each bacterium (7.7 log CFU/mL) was grown in 5% (v/v) MRS containing 100 μg/mL of ferric sulfate, and time-dependent changes of ferrous ion level in culture broth of 5 LAB (A) or L. kimchicus (B) were analyzed during 72 h. Ferrous ion levels produced by viable and autoclaved L. kimchicus were also compared after 24 h incubation (C). Each value represents the mean±SD (n=3). Different letters indicate a significant difference (p<0.05) based on one way ANOVA and the Tukey's HSD. ** indicates a significant difference (p<0.01) based on Student’s t-test (in C).
표 Table 1. Reducing abilities on ferric to ferrous ion by different LAB
그림 Fig. 4. Radical scavenging activities of LAB bacterial lysates and intact LAB. Scavenging activities of LAB lysates against ABTS (A) and DPPH (B) radicals were analyzed. ABTS radical scavenging activity of intact LAB was also analyzed (C). Each value represents the mean±SD (n=3). Different letters indicate a significant difference (p<0.05) based on one way ANOVA and the Tukey's HSD test.

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