Lim, Dahye
(Department of Veterinary Dermatology, College of Veterinary Medicine, Kyungpook National University)
,
Bae, Seulgi
(Department of Veterinary Dermatology, College of Veterinary Medicine, Kyungpook National University)
,
Oh, Taeho
(Department of Veterinary Dermatology, College of Veterinary Medicine, Kyungpook National University)
Shea butter (Vitellaria paradoxa) is a fat extracted from shea tree nuts and contains relatively high levels of non-glycerides. Triterpenes, the main non-glyceride component, exhibit a variety of biological activities such as antitumor, antibacterial, and anti-inflammatory. Shea butter extract (SBE)...
Shea butter (Vitellaria paradoxa) is a fat extracted from shea tree nuts and contains relatively high levels of non-glycerides. Triterpenes, the main non-glyceride component, exhibit a variety of biological activities such as antitumor, antibacterial, and anti-inflammatory. Shea butter extract (SBE) has been used to treat various skin problems such as burns, eczema, and rash in human medicine, but little is known about the activity of SBE on canine skin. This study evaluated the cytotoxicity and anti-inflammatory effect of SBE in canine keratinocytes. Cytotoxicity of lipopolysaccharide (LPS, 5-50 ng/mL) and SBE (50-200 ㎍/mL) was evaluated using the CCK-8 assay. Non-cytotoxic concentrations of LPS and SBE were administered to canine cell cultures to evaluate anti-inflammatory effects. To evaluate the anti-inflammatory activity of SBE, the levels of IL-1β, IL-8, IL-12, and TNF-α were measured using ELISA kits. The concentration of each cytokine was quantified in control, LPS-treated, LPS + SBE-treated groups. Increased levels of IL-1β, IL-8, and IL-12 were found in LPS-treated groups relative to control groups. LPS + SBE-treated groups showed a lower level of IL-1β, IL-8, and IL-12 than LPS-treated groups. These results suggest that SBE may have application as a topical agent for canine inflammatory skin diseases. However, further in vivo study is needed to evaluate the safety and efficacy of SBE in dogs.
Shea butter (Vitellaria paradoxa) is a fat extracted from shea tree nuts and contains relatively high levels of non-glycerides. Triterpenes, the main non-glyceride component, exhibit a variety of biological activities such as antitumor, antibacterial, and anti-inflammatory. Shea butter extract (SBE) has been used to treat various skin problems such as burns, eczema, and rash in human medicine, but little is known about the activity of SBE on canine skin. This study evaluated the cytotoxicity and anti-inflammatory effect of SBE in canine keratinocytes. Cytotoxicity of lipopolysaccharide (LPS, 5-50 ng/mL) and SBE (50-200 ㎍/mL) was evaluated using the CCK-8 assay. Non-cytotoxic concentrations of LPS and SBE were administered to canine cell cultures to evaluate anti-inflammatory effects. To evaluate the anti-inflammatory activity of SBE, the levels of IL-1β, IL-8, IL-12, and TNF-α were measured using ELISA kits. The concentration of each cytokine was quantified in control, LPS-treated, LPS + SBE-treated groups. Increased levels of IL-1β, IL-8, and IL-12 were found in LPS-treated groups relative to control groups. LPS + SBE-treated groups showed a lower level of IL-1β, IL-8, and IL-12 than LPS-treated groups. These results suggest that SBE may have application as a topical agent for canine inflammatory skin diseases. However, further in vivo study is needed to evaluate the safety and efficacy of SBE in dogs.
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제안 방법
To evaluate the relative anti-inflammatory effect of SBE, further study is needed. Furthermore, this study evaluated the anti-inflammatory effect of SBE against several cytokines and was performed in vitro. Further study to evaluate the safety and clinical effectiveness of SBE in canines is needed.
In human medicine, shea butter has been used as a topical agent in skincare and to treat various skins disorders. In this study, we evaluated the cytotoxicity and anti-inflammatory effects of SBE in canine epidermal progenitor keratinocytes (CPEK).
데이터처리
0 (Systat Software; USA). Differences between groups were assessed with one-way ANOVA. P-values<0.
이론/모형
In this study, we evaluated the cytotoxicity and anti inflammatory effects of SBE in lipopolysaccharide (LPS)-induced inflammation in canine keratinocytes, using the CCK-8 assay and ELISA.
The cytotoxic effects of LPS and SBE in CPEK cells were assessed using the CCK-8 assay. Based on the results of the CCK-8 assays, the concentrations of LPS and SBE used to treat cells before ELISA assays were selected.
The cytotoxicity of LPS in CPEK cells was evaluated using the CCK-8 assay (Table 1). Table 1.
The levels of IL-1, IL-8, IL-12, and TNF- were measured to evaluate the anti-inflammatory effect of SBE in LPS-induced inflammation of CPEK cells using ELISA assays. The CPEK cells were divided into Control (no treatment), LPS-treated, and LPS+SBE-treated groups.
성능/효과
in LPS-activated macrophage cells (30). This study revealed that triterpenes, the main non-glyceride component in SBE, suppressed the NF-B nuclear translocation induced by LPS (30). LPS is a component of Gram-negative bacteria and triggers an inflammatory response (30).
후속연구
Furthermore, this study evaluated the anti-inflammatory effect of SBE against several cytokines and was performed in vitro. Further study to evaluate the safety and clinical effectiveness of SBE in canines is needed.
However, a comparison between conventional anti inflammatory agent and SBE is not performed in this study. To evaluate the relative anti-inflammatory effect of SBE, further study is needed. Furthermore, this study evaluated the anti-inflammatory effect of SBE against several cytokines and was performed in vitro.
참고문헌 (30)
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