[논문]Penicillium ulleungdoense sp. nov. from Ulleung Island in Korea

Choi, Doo-Ho; You, Young-Hyun; Lee, In-Seon; Hong, Seung-Bum; Jung, Tea-Yeol; Kim, Jong-Guk

doi:10.1080/12298093.2020.1852702

[국내논문] Penicillium ulleungdoense sp. nov. from Ulleung Island in Korea 원문보기

Mycobiology, v.49 no.1, 2021년, pp.46 - 53

Choi, Doo-Ho (School of Life Science and Biotechnology, Kyungpook National University) , You, Young-Hyun (Microorganism Resources Division, National Institute of Biological Resources) , Lee, In-Seon (Department of Food Science and Technology, Keimyung University) , Hong, Seung-Bum (Korean Agricultural Culture Collection, National Institute of Agricultural Science, RDA) , Jung, Tea-Yeol (Department of Landscape Architecture, Kyungpook National University) , Kim, Jong-Guk (School of Life Science and Biotechnology, Kyungpook National University)

Abstract ▼ AI-Helper

In a study of the fungal diversity on Ulleung Island in Korea, three novel strains of Penicillium were isolated. Different sites on Ulleung Island were selected for collecting endophytic fungi, and three endophytic fungal strains showed unique morphological characteristics. DNA sequence of the internal transcribed spacer, β-tubulin, calmodulin, and RNA polymerase II second largest subunit regions of the strains were analyzed and they showed unique taxonomic position from the other species of Penicillium section Sclerotiora. The new strains were named Penicillium ulleungdoense sp. nov. As the novel endophytic Penicillium taxa were discovered in a unique environment, the data could be meaningful for understanding the geographical distribution of Ascomycetes on Ulleung Island.

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표/그림 (5)

표 Table 1. Details of the strains used in phylogenetic analyses.
그림 Figure 1. Phylogenetic tree based on the maximum likelihood analysis of the combined ITS, BenA and CaM dataset for species classified in the Penicillium section. Penicillium levitum was included as an outgroup. Bootstrap analysis was performed with 1,000 replications. Bootstrap support values of ≥ 70% are indicated at the nodes. The bar indicates the number of substitutions per position. T indicates the type strains of the species. Bar, 0.02 substitutions per nucleotide position. The isolates KACC 48990, KACC 48991 and KACC 48992 are marked in red.
그림 Figure 2. Phylogenetic tree based on the maximum likelihood analysis of RPB2 dataset for species classified in the Penicillium section. Penicillium levitum was included as an outgroup. Bootstrap analysis was performed with 1,000 replications. Bootstrap support values of ≥ 70% are indicated at the nodes. The bar indicates the number of substitutions per position. T indicates the type strains of the species. Bar, 0.02 substitutions per nucleotide position. The isolates KACC 48990, KACC 48991 and KACC 48992 are marked in red.
그림 Figure 3. Morphological characteristics of Penicillium ulleungdoense (KACC 48990) grown on different media. (A) CYA 25 ºC, (B) CYA 30 ºC, (C) CYA 37 ºC, (D) MEA 25 ºC, (E) YESA 25 ºC, (F) CYAS 25 ºC, and (G) CZ 25 ºC (top: obverse, bottom: reverse); (H) Colony on OA 25 ºC; (I) Colony on CREA 25 ºC; (J–N) Conidiophores; (O) Conidia (Scale bar = 50 μm in J=K. Scale bar = 10 μm in L-O).
표 Table 2. Morphological characteristics of KACC 48990 and the reference species Penicillium hirayamae on Czapek yeast autolysate agar (CYA) at 25 ºC.

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of Korea. Gyeongsang Province, Ulleung Island, 37^(cid:1)14’33.29"N₁₃1^(cid:1)51’53.43"E, Sedumoryzifo- lium, 4 April 2017, D.H. Choi, J.G. Kim; KACC 48992, Republic of Korea. Gyeongsang Province, UlleungIsland, 37^(cid:1)14’20.
TherootsofPhedimus takesimensis (E130^(cid:1)54’32.76", N₃₇^(cid:1)28’54.97"), Sedumoryzifolium(E130^(cid:1)47’55.45", N 37^(cid:1)31’07.27"), and Aster spathulifolius (E130^(cid:1)53’15.88", N₃₇^(cid:1)32’29.09")werecollectedfrom Ulleung Island in 4 April 2017. The root samples werewashedwithdistilledwatertoremovesandpar- ticlesandtreatedwithTween-80solutionfor5min.
Colonydiam, 7d, 25^(cid:1)C(unlessstatedotherwise): CYA16–18mm;CYA30^(cid:1)C₂₅–28mm;CYA37^(cid:1)C 4–7mm; MEA 12–17mm; YES 19–24mm; CREA 6–10mm;CZ8–9mm;OA13–16mm.
The culture is preserved in Korean Agricultural Culture Collection (KACC) in Jeonju, Korea. Molecular markers for the species are MN₆₄0087for rDNA–ITS, MN₇₃7487forb–tubulin, MN₇₄5074for calmodulin, andMN₇₅6007forRNA polymeraseIIsecondlargestsubunit.
2017. The culture is preserved in Korean Agricultural Culture Collection (KACC) in Jeonju, Korea. Molecular markers for the species are MN₆₄0087for rDNA–ITS, MN₇₃7487forb–tubulin, MN₇₄5074for calmodulin, andMN₇₅6007forRNA polymeraseIIsecondlargestsubunit.
The isolatedfungal strains fromthe roots were subcultured on potato dextrose agar (PDA)andincubatedat25^(cid:1)Cforaweekinthedark toallowfungal growth[22].The isolatesKMG401, KMG402, and KMG403 were deposited to the KoreanAgriculturalCultureCollection(KACC)with allottedno.KACC₄₈990, KACC₄₈991, andKACC 48992, respectively.
Amplifications were performed withdifferentprimersetsina20μlreactionmixture [23, 24]. The products were purified using the QIAquick PCR Purification Kit (Qiagen, Hilden, Germany) and sequenced using the ABI PRISM BigDye Terminator Cycle Sequencing Kit (PE Biosystems, Foster, CA, USA) with the ABI 310 DNAsequencer(PEBiosystems)[8].
1). The target strainsKACC₄₈990, KACC₄₈991, and KACC₄₈992werepositionedusingthesequencesof theITSregion, BenAregion, CaMregion, andRPB2 region(Figures1and2).Basedonthesefourgenes, the target strainswere comparedwithother strains available intheNCBIdatabase.
TheDNAsequencesof thenewspecieswereregistered in the GenBank database of the NCBI (MN₆₄0087–MN₆₄0089for rDNA–ITS, MN₇₃7487– MN₇₃7489forBenA, MN₇₄5074–MN₇₄5076forCaM, and MN₇₅6007–MN₇₅6009 for RPB2) (Table 1). The target strainsKACC₄₈990, KACC₄₈991, and KACC₄₈992werepositionedusingthesequencesof theITSregion, BenAregion, CaMregion, andRPB2 region(Figures1and2).

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