[논문]금은화에서 분리한 Lonicerin의 신경세포보호 활성

이현우; 마충제

doi:10.22889/kjp.2021.52.1.19

금은화에서 분리한 Lonicerin의 신경세포보호 활성
Neuroprotective Activity of Lonicerin Isolated from Lonicera japonica 원문보기

생약학회지, v.52 no.1, 2021년, pp.19 - 25

이현우 (강원대학교 의생명과학대학 생물의소재공학과) , 마충제 (강원대학교 의생명과학대학 생물의소재공학과)

Abstract ▼ AI-Helper

We previously reported that lonicerin isolated from Lonicera japonica methanolic extract had potent neuro-protective activities in neuronal cell death injured by excessive glutamate. In this study, we tried to confirm the neuroprotective activities of L. japonica extract and lonicerin in glutamate injured HT22 cells and establish mechanisms of neuroprotective action of lonicerin. We used HT22 cell death injured by glutamate as a bioassay system. The compound decreased reactive oxygen species increased by excessive glutamate treatment in HT22 cells. Also, Ca2+ concentration was decreased by lonicerin treatment. This compound made mitochondrial membrane potential maintain to normal condition. Lonicerin also increased not only glutathione reductase but also peroxidase to the control level. And this compound increased amount of glutathione, an endogenous antioxidant. These results indicated that lonicerin isolated from L. japonica showed potent neuroprotective activity through the anti-oxidative pathway.

주제어

표/그림 (7)

그림 Fig. 1. Structure of lonicerin isolated from L. japonica.
그림 Fig. 2. Effect of lonicerin (1.0, 10.0 and 100.0 μM) on glu-tamate- induced death of HT22 cells. Data expressed as mean ± the standard error of the mean. *p<0.05, **p<0.01, ***p< 0.001 versus the glutamate-treated group.
그림 Fig. 3. Effect of lonicerin (1.0, 10.0 and 100.0 μM) on reac-tive oxygen species production in glutamate injured HT22 cells. Data expressed as mean ± the standard error of the mean. *p<0.05, **p<0.01, ***p<0.001 versus the glutamate-treated group.
그림 Fig. 4. Effect of lonicerin (1.0, 10.0 and 100.0 μM) on cal-cium ion influx in glutamate injured HT22 cells. Data expressed as mean ± the standard error of the mean. *p<0.05, **p<0.01, ***p<0.001 versus the glutamate-treated group.
그림 Fig. 5. Effect of lonicerin (1.0, 10.0 and 100.0 μM) on glu-tamate- induced disruption of mitochondrial membrane poten-tial in HT22 cells. Data expressed as mean ± the standard error of the mean. *p<0.05, **p<0.01, ***p<0.001 versus the glutamate-treated group.
그림 Fig. 6. Effect of lonicerin (1.0, 10.0 and 100.0 μM) on glu-tathione level in glutamate injured HT22 cells. Data expressed as mean ± the standard error of the mean. *p<0.05, **p<0.01, ***p<0.001 versus the glutamate-treated group.
그림 Fig. 7. Effect of lonicerin (1.0, 10.0 and 100.0 μM) on glu-tathione reductase (A) and glutathione peroxidase (B) in glu-tamate injured HT22 cells. Data expressed as mean ± the standard error of the mean. *p<0.05, **p<0.01, ***p<0.001 versus the glutamate-treated group.

AI 본문요약
AI-Helper

문제 정의

본 연구에서는 이미 HT22 세포주에서 신경세포 보호 활성이 보고된 금은화 추출물로부터 분리 정제한 lonicerin의신경세포 보호 활성을 다시 한번 확인하고 생리활성의 작용기전을 밝히고자 하였다. glutamate로 신경 독성을 유도한 HT22 세포주를 활성 검색계로 하여 신경 세포의 사멸과 관련된 바이오 마커의 변화를 평가하여 lonicerin의 약리작용 기전을 규명하는 연구를 수행하였다.
본 연구에서는 이미 HT22 세포주에서 신경세포 보호 활성이 보고된 금은화 추출물로부터 분리 정제한 lonicerin의신경세포 보호 활성을 다시 한번 확인하고 생리활성의 작용기전을 밝히고자 하였다. glutamate로 신경 독성을 유도한 HT22 세포주를 활성 검색계로 하여 신경 세포의 사멸과 관련된 바이오 마커의 변화를 평가하여 lonicerin의 약리작용 기전을 규명하는 연구를 수행하였다.

참고문헌 (33)

Pizarro, I. V., Swain, G. P. and Selzer, M. E. (2004) Cell proliferation in the lamprey central nervous system. J. Comp. Neurol. 469: 298-310.

상세보기
Brewster, J. L., Linseman, D. A., Bouchard, R. J., Loucks, F. A., Precht, T. A., Esch, E. A. and Heidenreich, K. A. (2006) Endoplasmic reticulum stress and trophic factor withdrawal activate distinct signaling cascades that induce glycogen synthasekinase-3 band a caspase-9-dependent apoptosis in cerebellar granule neurons. Mol. Cell. Neurosci. 32: 242-253.

상세보기
Ryu, E. J., Harding, H. P., Angelastro, J. M., Vitolo, O. V., Ron, D. and Greene, L. A. (2002) Endoplasmic reticulum stress and the unfolded protein response in cellular models of Parkinson's disease. J. Neurosci. 22: 10690-10698.

상세보기
Steckley, D., Karajgikar, M., Dale, L. B., Fuerth, B., Swan, P., Drummond-Main, C.,Poulter, M. O., Ferguson, S. S. G., Strasser, A. and Cregan, S. P. (2007) Puma is adominant regulator of oxidative stress induced Bax activation and neuronal apoptosis. J. Neurosci. 27: 12989-12999.

상세보기
Bradley, M. A., Markesbery, W. R. and Lovell, M. A. (2010) Increased levels of 4-hydroxynonenal and acrolein in the brain in preclinical Alzheimer disease. Free Rad. Biol. Med. 48: 1570-1576.

상세보기
Mattson, M. P. (2004) Pathways towards and away from Alzheimer's disease. Nature 430: 631-639.

상세보기
Seidler, N. W. and Squire, T. J. (2005) A beta-polyacrolein aggregates: novel mechanism of plastic formation in senile plaques. Biochem. Biophys. Res. Comm. 335: 501-504.

상세보기
Kuhla, B., Haase, C., Flach, K., Luth, H. J., Arendt, T. and Munch, G. (2007) Effect of pseudophosphorylation and cross-linking by lipid peroxidation and advanced glycation end product precursors on tau aggregation and filament formation. J. Biol. Chem. 282: 6984-6991.

상세보기
Srivastava, S., Sithu, S. D., Vladykovskaya, E., Haberzettl, P., Hoetker, D. J., Siddiqui, M. A., Conklin, D. J., D'Souza, S. E. and Bhatnagar, A. (2011) Oral exposure to acrolein exacerbates atherosclerosis in apoE-null mice. Atherosclerosis 215: 301-308.

상세보기
Sultana, R. and Butterfield, D. A. (2010) Role of oxidative stress in the progression of Alzheimer's disease. J. Alzheimer's Dis. 19: 341-353.

상세보기
Weon, J. B., Yang, H. J., Lee, B., Yun, B-. R., Ahn, J. H., Lee, H. Y. and Ma, C. J. (2011) Neuroprotective activity of the methanolic extract of Lonicera japonica in glutamate-injured primary rat cortical cells. Pharmacog. Mag. 7: 284-288.

상세보기
Weon, J. B., Yang, H. J., Lee, B., Yun, B-. R. and Ma, C. J. (2011) Neuroprotective compounds isolated from the methanolic extract of Lonicera japonica. Nat. Prod. Sci. 17: 221-224.
Lin, M. T. and Beal, M. F. (2006) Mitochondrial dysfunction and oxidative stress in neurodegenerative diseases. Nature 443: 787-795.

상세보기
Kim, M. S., Seo, J. Y., Oh, J., Jang, Y. K., Lee, C. H. and Kim, J. S. (2017) Neuroprotective effect of halophyte Salicornia herbacea L. is mediated by activation of heme oxygenase-1 in mouse hippocampal HT22 cells. J. Med. Food 20: 140-151.

상세보기
Yan, M. H., Wang, X. L. and Zhu, X. W. (2013) Mitochondrial defects and oxidative stress in Alzheimer disease and Parkinson disease. Free Rad. Biol. Med. 62: 90-101.

상세보기
Carrano, A., Hoozemans, J. J. M., van der Vies, S. M., Rozemuller, A. J. M., van Horssen, J. and de Vries, H. E. (2011) Amyloid beta induces oxidative stress-mediated blood-brain barrier changes in capillary amyloid angiopathy. Antioxid. Redox. Signal 15: 1167-1178.

상세보기
Helmut, S. (1999) Glutathione and its role in cellular functions. Free Radic. Biol. Med. 27: 916-921.

상세보기
Qi, L-W., Chen, C-Y. and Li, P. (2009) Structural characterization and identification of iridoid glycosides, saponins, phenolic acids and flavonoids in Flos Lonicerae Japonicae by a fast liquid chromatography method with diode-array detection and time-of-flight mass spectrometry. Rapid Comm. Mass Spectrom. 23: 3227-3242.

상세보기
Jung, Y. S., Weon, J. B., Yang, W. S., Ryu, G. and Ma, C. J. (2018) Neuroprotective effects of Magnoliae Flos extract in mouse hippocampal neuronal cells. Sci. Rep. 8: 9693.

상세보기
Meldrum, B. S. (2002) Concept of activity-induced cell death in epilepsy: historical and contemporary perspectives. Prog. Brain Res. 135: 3-11.

상세보기
Coyle, J. T. and Puttfarcken, P. (1993) Oxidative stress, glutamate, and neurodegenerative disorders. Science. 262: 689-695.

상세보기
Ferreiro, E., Balderias, I., Ferreira, I. L., Costa, R. O., Rego, A. C., Pereira, C. F. and Oliveira, C. R. (2012) Mitochondrial- and endoplasmic reticulum-associated oxidative stress in Alzheimer's disease: from pathogenesis to biomarkers. Int. J. Cell Biol. 2012: 735206.

상세보기
Armstead, W. M., Mirro, R., Leffler, C. W. and Busija, D. W. (1989) Cerebral superoxide anion generationduring seizures in newborn pigs. J. Cereb. Blood Flow Metab. 9: 175-179.

상세보기
Parfenova, H., Carratu, P., Tcheranova, D., Fendinec, A., Pourcyrous, M. and Leffler, C. W. (2005) Epileptic seizures cause extended postictal cerebral vascular dysfunction that is prevented by HO-1 overexpression. Heart Cir. Physiol. 288: H2843-2850.

상세보기
Lee, Y., Shin, D. H., Kim, J. H., Hong, S., Choi, D., Kim, Y. J., Kwak, M. K. and Jung, Y. (2010) Caffeic acid phenethyl ester mediated Nrf2 activation and IkappaB kinase inhibition are involved in NFkappaB inhibitory effect: structural analysis for NFkappaB inhibition. Eur. J. Pharmacol. 643: 21-28.

상세보기
Li, M. H., Inoue, K., Si, H. F. and Xiong, Z. H. (2011) Calcium-permeable ion channels involved in glutamate receptorindependent ischemic brain injury. Acta Pharmacol. Sin. 32: 734-740.

상세보기
Ward, M. W., Rego, A. C., Frenguelli, B. G and Nicholls, D. G. (2000) Mitochondrial membrane potential and glutamate exicitotoxicity in cultured cerebellar granule cells. J. Neurosci. 20: 7208-7219.

상세보기
Kushairi, N., Phan, C. W., Sabaratnam, V., David, P. and Naidu, M. (2019) Lion's mane mushroom, Hericium erinaceus (Bull.: Fr.) pers. suppresses H 2 O 2 -induced oxidative damage and LPS-induced inflammation in HT22 hippocampal neurons and BV2 microglia. Antioxidants 8: 261.

상세보기
Xu, F., Cao, S., Wang, C., Wang, K., Wei, Y., Shao, X. and Wang, H. (2019) Antimicrobial activity of flavonoids from Sedum aizoon L. against Aeromonas in culture medium and in frozen pork. Food Sci. Nutr. 7: 3224-3232.

상세보기
Scaria, B., Sood, S., Raad, C., Khanafer, J., Jayachandiran, R., Pupulin, A., Grewal, S., Okoko, M., Arora, M., Miles, L. and Pandey, S. (2020) Natural Health Products (NHP's) and natural compounds as therapeutic agents for the treatment of cancer; mechanisms of anti-cancer activity of natural compounds and overall trends. Int. J. Mol. Sci. 21: 8480.

상세보기
Choudhary, M., Kumar, V., Malhotra, H. and Singh, S. (2015) Medicinal plants with potential anti-arthritic activity. J. Intercult. Ethnopharmacol. 4: 147-179.

상세보기
Lin, Y-P., Chen, T-Y., Tseng, H-W., Lee, M-H. and Chen, ST. (2009) Neural cell protective compounds isolated from Phoenix hanceana var. formosana. Phytochem. 70: 1173-1181.

상세보기
Kim, N. M., Kim, J., Chung, H. Y. and Choi, J. S. (2000) Isolation of luteolin 7-O-rutinoside and esculetin with potential antioxidant activity from the aerial parts of Artemisia montana. Arch. Pharm. Res. 23: 237-239.

원문보기 상세보기

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증