[논문]콩제비꽃 전초 추출물의 모유두세포 증식 기전

강정일; 서민정; 최윤경; 신수영; 황용; 고재덕; 유은숙; 김상철; 강희경

doi:10.22889/kjp.2021.52.1.34

콩제비꽃 전초 추출물의 모유두세포 증식 기전
The Mechanism of Whole Plant Extract of Viola verecunda on the Proliferation of Dermal Papilla Cells 원문보기

생약학회지, v.52 no.1, 2021년, pp.34 - 40

강정일 (제주대학교 의학전문대학원 의학과) , 서민정 (국립낙동강생물자원관 담수생물연구본부) , 최윤경 (제주대학교 의학전문대학원 의학과) , 신수영 (국립낙동강생물자원관 담수생물연구본부) , 황용 (국립낙동강생물자원관 담수생물연구본부) , 고재덕 (국립낙동강생물자원관 담수생물연구본부) , 유은숙 (제주대학교 의학전문대학원 의학과) , 김상철 (국립낙동강생물자원관 담수생물연구본부) , 강희경 (제주대학교 의학전문대학원 의학과)

Abstract ▼ AI-Helper

Proliferation and maintain of dermal papilla during progression of hair-cycle are crucial to the duration of anagen and regulated by diverse signaling pathway such as PI3K/Akt/Wnt/β-catenin pathway. In this study, we investigated the effects and mechanisms of Viola verecunda on dermal papilla cells. Treatment of dermal papilla cells with whole plant extract of V. verecunda resulted in cell proliferation, which was accompanied by up-regulation of cyclin D1, phospho (ser780)-pRB and cdc2 p34, and down-regulation of p27kip1. V. verecunda extract also promoted the levels of phospho (ser473)-Akt and phospho (ser780)-pRB in a time-dependent manner. Inhibition of PI3K/Akt by Wortmannin suppressed progression of cell-cycle, thereby attenuated the increases in proliferation of dermal papilla cells by V. verecunda extract. We further investigated Wnt/β-catenin pathway with respect to the effects of V. verecunda extract on the proliferation of dermal papilla cells. Treatment with V. verecunda extract results in up-regulation of Wnt/β-catenin proteins such as phospho (ser9)-GSKβ, phospho (ser552)-β-catenin and phospho (ser675)-β-catenin. In addition, Wortmannin abrogated V. verecunda extract mediated up-regulation of cdc2 p34 and down-regulation of p27kip1. These finding reveal that the proliferative effect of V. verecunda mediated by alteration of cell-cycle via activating PI3K/Akt/Wnt pathway in dermal papilla cells.

주제어

표/그림 (3)

그림 Fig. 1. Viola verecunda extract increases the proliferation of dermal papilla cells. (A) The viability of the dermal papilla cells was determined by WST assay. The cells were treated with V. verecunda (0.1, 1, 10 and 100 μg/mL) extract or minoxidil (10 μM) for 48h. The proliferation of dermal papilla cells was evaluated compared to the vehicle-treated control. Data are presented as mean±SD from three independent exper-iments. **p<0.01 vs. vehicle-treated control. (B) The cells were stimulated with V. verecunda (0.1 μg/mL) extract or minoxidil (10 μM) for 24 h. The protein levels were analyzed by immunoblotting using specific antibodies. NT, not treated; DMSO, dimethyl sulfoxide.
그림 Fig. 2. V. verecunda extract promotes the proliferation of dermal papilla cells via activation of PI3K/Akt pathway. (A) The effects of V. verecunda extract on the levels of phospho(ser473)-Akt and phospho(ser780)-pRB. The cells were stimulated with 0.1 μg/mL of V. verecunda extract for 0-120 min, and the protein levels were analyzed by immunoblotting using specific antibodies. (B) The viability of the dermal papilla cells exposed to V. verecunda extract (0.1 μg/mL) with or without wortmannin (10 nM) for 48 h was determined by WST assay. *p<0.05 vs. vehicle-treated control; ††p<0.01 vs. wortmannin-treated group. (C) Quantitative comparison of cell cycle distribution induced by V. verecunda extract (0.1 μg/mL) in the presence or absence of wortmannin (10 nM) for 24 h. Data are presented as mean±SD from three independent experiments. *p<0.05, ***p<0.001 vs. vehicle-treated control, †††p< 0.001 vs. wortmannin-treated group.
그림 Fig. 3. V. verecunda extract activates the Wnt/β-catenin pathway in dermal papilla cells. (A) The effects of V. verecunda extract on the levels of Wnt/β-catenin proteins. The cells were stimulated with 0.1 μg/mL of V. verecunda extract for 0-120 min, and the pro-tein levels were analyzed by immunoblotting using specific antibodies. (B, C) The effects of V. verecunda extract on the localization of phospho(ser552)-β-catenin and phospho(ser675)-β-catenin. The dermal papilla cells were treated with wortmannin (10 nM) for 2 h prior to be stimulated with V. verecunda extract (0.1 μg/mL) for 1 h. The cells were stained with anti-phospho(ser552)-β-catenin, anti-phospho(ser675)-β-catenin and anti-α-Tubulin antibodies, and incubated with a corresponding Alexa Fluor® 488- or Alexa Fluor® 594-conjugated secondary antibody. Images were obtained using FV1200 confocal Microscope. The scale bar indi-cates 100 μm. (D) The effects of V. verecunda extract on the levels of cdc2 p34 and p27^kip1. The cells were treated with wort-mannin (10 nM) for 2 h prior to be stimulated with 0.1 μg/mL of V. verecunda extract for 24 h. Immunoblotting was performed with anti-cdc2 p34 and p27^kip1 antibodies.

AI 본문요약
AI-Helper

문제 정의

본 연구에서는 모발의 성장에서 중요한 역할을 하는 모유두세포에서 콩제비꽃의 효능 및 작용기전을 조사하여 이를 탈모방지제 및 치료제로 이용할 수 있는 근거를 제시하고자 하였다.
본 연구에서는 콩제비꽃 추출물이 육모 효능이 있는지 조사하고자 모발의 성장에서 중요한 역할을 하는 모유두세포에서 콩제비꽃의 효능 및 작용기전을 조사하였다.

대상 데이터

콩제비꽃 추출물의 제조-실험재료인 콩제비꽃(Viola verecunda)은 경북 상주 부근에서 채취 후 국립낙동강생물자원관 황용 박사가 동정하였고, 증거 표본(NNIBRVP 69077)은 국립낙동강생물자원관에 보관되어 있다. 콩제비꽃 추출물을 다음과 같이 제조하여 사용하였다.

데이터처리

통계분석-모든 측정결과는 평균 ± 표준편차로 나타내었으며, 통계학적 유의성 검정은 unpaired student's t-test로 검정하였으며, p-value가 0.05이하일 경우 유의성을 인정하였다
05이하일 경우 유의성을 인정하였다. 통계처리는 Sigma Stat software(Jandel Scientific Software, USA)를 사용하였다.

성능/효과

콩제비꽃 추출물이 PI3K/Akt 및 Wnt/β-catenin 신호전달경로 조절을 통해 모발성장에서 중요한 모유두세포 증식 증가 효능을 나타내며, 콩제비꽃 추출물이 탈모치료 및 탈모 예방에 이용될 수 있다는 근거를 제시하는 것이다.

참고문헌 (27)

Price, V. H. (1999) Treatment of hair loss. N. Engl. J. Med. 341: 964-973.

상세보기
Ellis, J. A., Sinclair, R. and Harrap, S. B. (2002) Androgenetic alopecia: pathogenesis and potential for therapy. Expert. Rev. Mol. Med. 4: 1-11.

상세보기
Elliott, K., Stephenson, T. J. and Messenger, A. G. (1999) Differences in hair follicle dermal papilla volume are due to extracellular matrix volume and cell number: implications for the control of hair follicle size and androgen responses. J. Invest. Dermatol. 113: 873-877.

상세보기
Kaufman, K. D. (2002) Androgens and alopecia. Mol. Cell Endocrinol. 198: 89-95.

상세보기
Botchkarev, V. A. (2003) Molecular mechanisms of chemotherapy-induced hair loss. J. Investig. Dermatol. Symp. Proc. 8: 72-75.

상세보기
Batchelor, D. (2001) Hair and cancer chemotherapy: consequences and nursing care-a literature study. Eur. J. Cancer. Care (Engl). 10: 147-163.

상세보기
Aoki, E., Shibasaki, T. and Kawana, S. (2003) Intermittent foot shock stress prolongs the telogen stage in the hair cycle of mice. Exp. Dermatol. 12: 371-377.

상세보기
Kaufman, K. D. and Dawber, R. P. (1999) Finasteride, a Type 2 5alpha-reductase inhibitor, in the treatment of men with androgenetic alopecia. Expert. Opin. Investig. Drugs 8: 403-415.

상세보기
Han, J. H., Kwon, O. S., Chung, J. H., Cho, K. H., Eun, H. C. and Kim, K. H. (2004) Effect of minoxodil on proliferation and apoptosis in dermal papilla cells of human hair follicle. J. Dermatol. Sci. 34: 91-98.

상세보기
Messenger, A. G. and Rundegren, J. (2004) Minoxidil: mechanisms of action on hair growth. Br. J. Dermatol. 150: 186-194.

상세보기
Hamaoka, H., Minakuchi, K., Miyoshi, H., Arase, S., Chen, C. H. and Nakaya, Y. (1997) Effect of K + channel openers on K + channel in cultured human dermal papilla cells. J. Med. Invest. 44: 73-77.
Shorter, K., Farjo, N. P., Picksley, S. M. and Randall, V. A. (2008) Human hair follicles contain two forms of ATP-sensitive potassium channels, only one of which is sensitive to minoxidil. FASEB J. 22: 1725-1736.

상세보기
Kwack, M. H., Kang, B. M., Kim, M. K., Kim, J. C. and Sung, Y. K. (2011) Minoxidil activates beta-catenin pathway in human dermal papilla cells: A possible explanation for its anagen prolongation effect. J. Dermatol. Sci. 62: 154-159.

상세보기
Stenn, K. S. and Paus, R. (2001) Controls of hair follicle cycling. Physiol. Rev. 81: 449-494.

상세보기
Greco, V., Chen, T., Rendl, M., Schober, M., Pasolli, H. A., Stokes, N., Dela Cruz-Racelis, J. and Fuchs, E. (2009) A twostep mechanism for stem cell activation during hair regeneration. Cell Stem Cell 4: 155-169.

상세보기
Johnson, D. G. and Walker, C. L. (1999) Cyclins and cell cycle checkpoints. Annu. Rev. Pharmacol. Toxicol. 39: 295-312.

상세보기
Prall, O. W., Sarcevic, B., Musgrove, E. A., Watts, C. K. and Sutherland, R. L. (1997) Estrogen-induced activation of Cdk4 and Cdk2 during G1-S phase progression is accompanied by increased cyclin D1 expression and decreased cyclin-dependent kinase inhibitor association with cyclin E-Cdk2. J. Biol. Chem. 272: 10882-10894.

상세보기
Lee, S. I. and Jeong, J. G. (2012) A herbalogical study on the plants of Violaceae in Korea. Kor. J. Herbology 27: 77-83.
Moon, H. I., Jung, J. C. and Lee, J. (2007) Antiplasmodial activity of triterpenoid isolated from whole plants of Viola genus from South Korea. Parasitol. Res. 100: 641-644.

상세보기
Jahoda, C. A., Horne, K. A. and Oliver, R. F. (1984) Induction of hair growth by implantation of cultured dermal papilla cells. Nature 311: 560-562.

상세보기
Horne, K. A., Jahoda, C. A. and Oliver, R. F. (1986) Whisker growth induced by implantation of cultured vibrissa dermal papilla cells in the adult rat. J. Embryol. Exp. Morphol. 97: 111-124.

상세보기
Kang, J. I., Choi, Y. K., Koh, Y. S., Hyun, J. W., Kang, J. H., Lee, K. S., Lee, C. M., Yoo, E. S. and Kang, H. K. (2020) Vanillic acid stimulates anagen signaling via the PI3K/Akt/βcatenin pathway in dermal papilla cells. Biomol. Ther. (Seoul) 28: 354-360.

원문보기 상세보기
Sherr, C. J. and Roberts, J. M. (1999) CDK inhibitors: Positive and negative regulators of G1-phase progression. Genes Dev. 13: 1501-1512.

상세보기
Wangefjord, S., Brandstedt, J., Lindquist, K. E., Nodin, B., Jirstrom, K. and Eberhard, J. (2013) Associations of beta-catenin alterations and MSI screening status with expression of key cell cycle regulating proteins and survival from colorectal cancer. Diagn. Pathol. 8: 10.

상세보기
Hedgepeth, C. M., Conrad, L. J., Zhang, J., Huang, H. C., Lee, V. M. and Klein, P. S. (1997) Activation of the Wnt signaling pathway: a molecular mechanism for lithium action. Dev. Biol. 185: 82-91.

상세보기
Hino, S., Tanji, C., Nakayama, K. I. and Kikuchi, A. (2005) Phosphorylation of beta-catenin by cyclic AMP-dependent protein kinase stabilizes beta-catenin through inhibition of its ubiquitination. Mol. Cell. Biol. 25: 9063-9072.

상세보기
Monick, M. M., Carter, A. B., Robeff, P. K., Flaherty, D. M., Peterson, M. W. and Hunninghake, G. W. (2001) Lipopolysaccharide activates Akt in human alveolar macrophages resulting in nuclear accumulation and transcriptional activity of beta-catenin. J. Immunol. 166: 4713-4720.

상세보기

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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