[논문]펄스형 전자기장(PEMF) 자극을 이용한 DNCB로 유발된 아토피성 피부염 개선 효과

김준영; 이지영; 이자우; 이수경; 박창순; 양세정; 이용흠

doi:10.9718/jber.2021.42.2.48

펄스형 전자기장(PEMF) 자극을 이용한 DNCB로 유발된 아토피성 피부염 개선 효과
Effect on Improvement of Atopic Dermatitis Induced by DNCB using PEMF(Pulsed Electromagnetic Fields) Stimulation 원문보기

Journal of biomedical engineering research : the official journal of the Korean Society of Medical & Biological Engineering, v.42 no.2, 2021년, pp.48 - 54

김준영 (연세대학교 보건과학대학 의공학부, 의료전자기시스템 연구실) , 이지영 (연세대학교 보건과학대학 의공학부, 의료영상인공지능 연구실) , 이자우 (연세대학교 보건과학대학 의공학부, 의료전자기시스템 연구실) , 이수경 (연세대학교 보건과학대학 의공학부, 의료전자기시스템 연구실) , 박창순 (연세대학교 보건과학대학 의공학부, 의료전자기시스템 연구실) , 양세정 (연세대학교 보건과학대학 의공학부, 의료영상인공지능 연구실) , 이용흠 (연세대학교 보건과학대학 의공학부, 의료전자기시스템 연구실)

Abstract ▼ AI-Helper

Objective: The purpose of this study is to evaluate the improvement of atopic dermatitis induced by DNCB using pulsed electromagnetic fields (PEMF) stimulation through animal models. Methods: Twenty 6-week Hairless Mouse (SKH1) models were randomly divided into normal groups (n=5), PEMF 15 Hz groups (n=5), PEMF 75 Hz groups (n=5), and Sham groups (n=5). To induce uniform atopic dermatitis, the DNCB(2,4-Dinitrochlorobenzene) solution was applied for 6 weeks. During the process of skin inflammation, sensory evaluations were conducted every 3~4 days to grade symptoms of atopic dermatitis. Following the onset of atopic dermatitis symptoms, the PEMF stimulation (15 mT) in the PEMF group (15 Hz & / 75 Hz) was stimulated for 8 hours per day for 1 week. After all experiments, the dosal skin tissue was sectioned at 4 ㎛, and after H&E Staining, the skin tissue was observed through the light microscopy to analyze the skin lesion size. Results: As a result of the sensory evaluation, the difference in average sensory evaluation scores between PEMF groups (15 Hz & 75 Hz) was 0.4 points, indicating a low statistical significance. Between the PEMF 15 Hz Group and the Sham Group, the difference in average sensory evaluation scores was reduced to 1.6 points, resulting in high statistical significance (P<0.05) and the best effect. As a result of analyzing the size of the lesion, the difference in size of the lesion area between PEMF groups (15 Hz & 75 Hz) was 807 pixels, indicating a low statistical significance. Between the PEMF group (15 Hz & 75 Hz) and the Sham group, the size difference of the lesion area was 12946 pixels, 13753 pixels, respectively, indicating high statistical significance (P<0.001). It means that inflammation has improved in dorsal skin tissue. Conclusions: This study confirms that PEMF (15 Hz / 75 Hz, 15 mT) stimulation can improve symptoms of DNCB-induced atopic dermatitis.

주제어

표/그림 (6)

그림 그림 1. 펄스형 전자기장 자극 방법 Fig. 1. PEMF stimulation method
그림 그림 2. Matlab을 이용한 병변의 크기 분석 예시 Fig. 2. Example of analyzing the size of a lesion using Matlab (a) Original picture showing the lesion. (b) Photos with lesion masked after contrast adjustment. (c) a figure representing the size of a lesion as the number of pixels
그림 그림 3. 각 그룹별 시간경과에 따른 Clinical Score Fig. 3. Clinical Score by date for each group (a) Normal group applied AOO(Olive Oil : 100% Acetone = 1 : 3) solution and there was no pulsed electromagnetic field stimulation from 42 days to 48 days. (b) PEMF 15Hz group applied DNCB solution(AOO + DNCB) and stimulated the pulsed electromagnetic field to an average of 15 mT at 15 Hz from 42 days to 48 days. (c) PEMF 75 Hz group applied DNCB solution(AOO + DNCB) and stimulated the pulsed electromagnetic field to an average of 15 mT at 75 Hz from 42 days to 48 days. (d) Sham group applied DNCB solution(AOO + DNCB) and there was no pulsed electromagnetic field stimulation from 42 days to 48 days.
그림 그림 4. 각 그룹별 아토피 피부염 증상 변화 Fig. 4. Changes in atopic dermatitis symptoms for each group Photos of (a)14 days, (b)28 days, (c)34 days, (d)42 days, and (e)48 days of each group
그림 그림 5. 각 그룹별 시간경과에 따른 Clinical Score 분석 Fig. 5. Analysis of Clinical Score for each group on 48 days (p<0.05)
그림 그림 6. 각 그룹별 병변 영역 크기 분석 Fig. 6. Analysis of Lesion area for each group (p<0.001)

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