[논문]라카디브 해역 몰디브 자생 해조류 Padina boryana 추출물의 항산화 효능 평가

김현수; 이정민; 임미진; 고석천; 이효근; 제준건; 전유진; 이대성

doi:10.5657/kfas.2021.0162

라카디브 해역 몰디브 자생 해조류 Padina boryana 추출물의 항산화 효능 평가
Evaluation of the Antioxidant Efficacy of Padina boryana Extract from Maldivian Seaweed from Laccadive Sea Area 원문보기

한국수산과학회지 = Korean journal of fisheries and aquatic sciences, v.54 no.2, 2021년, pp.162 - 169

김현수 (국립해양생물자원관 유전자원실) , , (제주대학교 해양의생명과학부) , 이정민 (국립해양생물자원관 유전자원실) , 임미진 (국립해양생물자원관 유전자원실) , 고석천 (국립해양생물자원관 유전자원실) , 이효근 (제주대학교 해양의생명과학부) , 제준건 (제주대학교 해양의생명과학부) , 전유진 (제주대학교 해양의생명과학부) , 이대성 (국립해양생물자원관 유전자원실)

Abstract ▼ AI-Helper

Global warming has affected the distribution of organisms for decades and has displayed rapid ascent recently. Research into the effects on tropical organisms are vital. Padina boryana is a resourceful marine microalgae in the Maldives Sea in the Laccadive region. A 70% ethanol extraction (PBE) of this seaweed was used to investigate its antioxidant potential. Both in vitro and in vivo models were implemented. PBE exhibited protective potential against H2O2 induced apoptosis. ROS levels were suppressed due to PBE. PBE expressed a cytoprotective nature. In vivo experiments involving the zebra fish model conformed its validity. The antioxidant efficacy of PBE was dose dependent. Study outcomes suggest PBE has potential as a novel and valuable marine resource to aid the functional food and cosmeceutical industries.

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표/그림 (6)

그림 Fig. 1. Protective effects of PBE against H₂O₂-induced cell death in vero cells (A) and intracellular reactive oxygen species (ROS) scavenging activities of PBE against H₂O₂-induced oxidative stress in vero cells (B). *P<0.05, **P<0.01 as compared to the H₂O₂-treated group and ^##P<0.01 as compared to the control group. PBE, 70% ethanol extract.
그림 Fig. 2. The apoptotic body formation was observed under a fluorescence microscope after Hoechst 33342 staining.
그림 Fig. 3. The survival rate (A) and heart-beating rate (B) after pretreatment with or without PBE in zebrafish Danio rerio exposed to H₂O₂. The experiments were conducted in triplicate, and the data were expressed as the mean±standard error (S.E). *P<0.05, **P<0.01 as compared to the H₂O₂-treated group and ^##P<0.01 as compared to the control group. PBE, 70% ethanol extract.
그림 Fig. 4. Protective effect of PBE on cell death in H₂O₂-induced zebrafish Danio rerio embryos. Fluorescence intensity of images was measured using the ImageJ software. The experiments were conducted in triplicate, and the data were expressed as the mean±standard error (S.E). *P<0.05, **P<0.01 as compared to the H₂O₂-treated group and ^##P>0.01 as compared to the control group. PBE, 70% ethanol extract.
그림 Fig. 5. Protective effect of PBE on reactive oxygen species (ROS) in H₂O₂-induced zebrafish Danio rerio embryos. Fluorescence intensity of images was measured using the ImageJ software. The experiments were conducted in triplicate, and the data were expressed as the mean±standard error (S.E). *P<0.05, **P<0.01 as compared to the H₂O₂-treated group and ^##P>0.01 as compared to the control group. PBE, 70% ethanol extract.
그림 Fig. 6. Protective effect of PBE on lipid peroxidation in H₂O₂-induced zebrafish Danio rerio embryos. Fluorescence intensity of images was measured using the ImageJ software. The experiments were conducted in triplicate, and the data were expressed as the mean±standard error (S.E). *P<0.05, **P<0.01 as compared to the H₂O₂-treated group and ^##P>0.01 as compared to the control group. PBE, 70% ethanol extract.

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