[논문]Characteristics of Bacteriophage Isolates and Expression of Shiga Toxin Genes Transferred to Non Shiga Toxin-Producing E. coli by Transduction

Park, Da-Som; Park, Jong-Hyun

doi:10.4014/jmb.2102.02040

Characteristics of Bacteriophage Isolates and Expression of Shiga Toxin Genes Transferred to Non Shiga Toxin-Producing E. coli by Transduction 원문보기

Journal of microbiology and biotechnology, v.31 no.5, 2021년, pp.710 - 716

Park, Da-Som (Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University) , Park, Jong-Hyun (Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University)

Abstract ▼ AI-Helper

A risk analysis of Shiga toxin (Stx)-encoding bacteriophage was carried out by confirming the transduction phage to non-Stx-producing Escherichia coli (STEC) and subsequent expression of the Shiga toxin genes. The virulence factor stx1 was identified in five phages, and both stx1 and stx2 were found in four phages from a total of 19 phage isolates with seven non-O157 STEC strains. The four phages, designated as ϕNOEC41, ϕNOEC46, ϕNOEC47, and ϕNOEC49, belonged morphologically to the Myoviridae family. The stabilities of these phages to temperature, pH, ethanol, and NaClO were high with some variabilities among the phages. The infection of five non-STEC strains by nine Stx-encoding phages occurred at a rate of approximately 40%. Non-STEC strains were transduced by Stx-encoding phage to become lysogenic strains, and seven convertant strains had stx1 and/or stx2 genes. Only the stx1 gene was transferred to the receptor strains without any deletion. Gene expression of a convertant having both stx1 and stx2 genes was confirmed to be up to 32 times higher for Stx1 in 6% NaCl osmotic media and twice for Stx2 in 4% NaCl media, compared with expression in low-salt environments. Therefore, a new risk might arise from the transfer of pathogenic genes from Stx-encoding phages to otherwise harmless hosts. Without adequate sterilization of food exposed to various environments, there is a possibility that the toxicity of the phages might increase.

주제어

참고문헌 (40)

1 Herold S Karch H Schmidt H 2004 Shiga toxin-encoding phages-genomes in motion Int. J. Med. Microbiol. 294 115 21 10.1016/j.ijmm.2004.06.023 15493821

상세보기
2 Calderwood SB Auclair F Donohue-Rolfe A Keusch GT Mekalanos JJ 1987 Nucleotide sequence of the Shiga-like toxin genes of Escherichia coli Proc. Natl. Acad. Sci. USA 84 4364 4368 10.1073/pnas.84.13.4364 3299365

상세보기
3 Jackson MP Neill RJ O'Brien AD Holmes RK Newland JW 1987 Nucleotide sequence analysis and comparison of the structural genes for Shiga-like toxin I and Shiga-like toxin II encoded by bacteriophages from Escherichia coli 933 FEMS. Microbiol. Lett. 44 109 114 10.1111/j.1574-6968.1987.tb02252.x

상세보기
4 Bielaszewska M Prager R Köck R Mellmann A Zhang W Tschäpe H 2007 Shiga toxin gene loss and transfer in vitro and in vivo during enterohemorrhagic Escherichia coli O26 infection in humans Appl. Environ. Microbiol. 73 3144 3150 10.1128/AEM.02937-06 17400784

상세보기
5 Elliott SJ Wainwright LA McDaniel TK Jarvis KG Deng Y Lai LC 1998 The complete sequence of the locus of enterocyte effacement (LEE) from enteropathogenic Escherichia coli Mol. Microbiol. 28 1 4 10.1046/j.1365-2958.1998.00783.x 9593291

상세보기
6 Perna NT Mayhew GF Pósfai G Elliott S Donnenberg MS Kaper JB 1998 Molecular evolution of a pathogenicity island from enterohemorrhagic Escherichia coli O157:H7 Infect. Immun. 66 3810 3817 10.1128/IAI.66.8.3810-3817.1998 9673266

상세보기
7 Karmali MA 2009 Host and pathogen determinants of verocytotoxin-producing Escherichia coli associated hemolytic uremic syndrome Kidney Int. 112 S4 S7 10.1038/ki.2008.608 19180132

상세보기
8 Croxen MA Law RJ Scholz R Keeney KM Wlodarska M Finlay BB 2013 Recent advances in understanding enteric pathogenic Escherichia coli Clin. Microbiol. Rev. 26 822 880 10.1128/CMR.00022-13 24092857

상세보기
9 Weitz JS Poisot T Meyer JR Flores CO Valverde S Sullivan MB 2012 Phage-bacteria infection networks Trends Microbiol. 21 82 91 10.1016/j.tim.2012.11.003 23245704

상세보기
10 Neely MN Friedman DI 1998 Arrangement and functional identification of genes in the regulatory region of lambdoid phage H-19B, a carrier of a Shiga-like toxin Gene 223 105 113 10.1016/S0378-1119(98)00236-4 9858702

상세보기
11 Johnson AD Poteete AR Lauer G Sauer RT Ackers GK Ptashne M 1981 λ repressor and cro-components of an efficient molecular switch Nature 294 217 223 10.1038/294217a0 6457992

상세보기
12 Fang Y Mercer RG McMullen LM Gänzle MG 2017 Induction of Shiga toxin-encoding prophage by abiotic environmental stress in food Appl. Environ. Microbiol. 83 e01378 17 10.1128/AEM.01378-17 28778890

상세보기
13 Ye WF Du M Zu MJ 2012 High temperature in combination with UV irradiation enhances horizontal transfer of stx2 gene from E. coli O157:H7 to non-pathogenic E. coli PLoS One 7 e31308 10.1371/journal.pone.0031308 22347461

상세보기
14 Los JM Los M Wegrzyn A Wegrzyn Z 2010 Hydrogen peroxide‐mediated induction of the Shiga toxin‐converting lambdoid prophage ST2‐8624 in Escherichia coli O157:H7 FEMS Immunol. Med. Microbiol. 58 322 329 10.1111/j.1574-695X.2009.00644.x 20070366

상세보기
15 Aertsen A De Spiegeleer P Vanoirbeek K Lavilla M Michiels CW 2005 Induction of oxidative stress by high hydrostatic pressure in Escherichia coli Appl. Environ. Microbiol. 71 2226 2231 10.1128/AEM.71.5.2226-2231.2005 15870304

상세보기
16 Krüger A Paula MA Lucchesi A 2014 Shiga toxins and stx phages: highly diverse entities Microbiol.-Reading 161 451 462 10.1099/mic.0.000003 25479836

상세보기
17 Wagner PL Waldor MK 2002 Bacteriophage control of bacterial virulence Infect. Immun. 70 3985 399 10.1128/IAI.70.8.3985-3993.2002 12117903

상세보기
18 Kropinski AM Mazzocco A Waddell TE Lingohr E Johnson RP Clokie MRJ Kropinski AM 2009 Enumeration of bacteriophages by double agar overlay plaque assay Bacteriophages, Vol. 1 Humana Press New York, USA 69 76 10.1007/978-1-60327-164-6_7
19 Klaenhammer TR McKay LL 1975 Isolation and examination of transducing bacteriophage particles from Streptococcus lactis C2 J. Dairy Sci. 59 396 404 10.3168/jds.S0022-0302(76)84219-1

상세보기
20 Kutter E Clokie MRJ Kropinski AM 2009 Phage host range and efficiency of plating Bacteriophages, Vol. 1 Humana Press New York, USA 141 149 10.1007/978-1-60327-164-6_14
21 Lee YD Kim JY Park JH 2013 Characteristics of coliphage ECP4 and potential use as a sanitizing agent for biocontrol of Escherichia coli O157:H7 Food Control 34 255 260 10.1016/j.foodcont.2013.04.043

상세보기
22 Amisano G Fornasero S Migliaretti G Caramello S Tarasco V Savino F 2011 Diarrheagenic Escherichia coli in acute gastroenteritis in infants in North-West Italy New Microbiologica 34 45 51 21344146

상세보기
23 Gerrish RS Lee JE Reed J Williams J Farrell LD Spiegel KM 2007 PCR versus hybridization for detecting virulence genes of enterohemorrhagic Escherichia coli Emerg. Infect. Dis. 13 1253 10.3201/eid1308.060428 17953106

상세보기
24 Sánchez S García-Sánchez A Martínez R Blanco J Blanco JE Blanco M 2009 Detection and characterisation of Shiga toxinproducing Escherichia coli other than Escherichia coli O157:H7 in wild ruminants Vet. J. 180 384 38 10.1016/j.tvjl.2008.01.011 18337133

상세보기
25 Schmidt H Beutin L Karch H 1995 Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157:H7 strain EDL 933 Infect. Immun. 63 1055 1061 10.1128/IAI.63.3.1055-1061.1995 7868227

상세보기
26 Hyman P Abedon ST Clokie MRJ Kropinski AM 2009 Practicla methods for determing phage growth papameters Phage host range and efficiency of plating Bacteriophages, Vol. 1 Humana Press New York, USA 175 202 10.1007/978-1-60327-164-6_18
27 Speirs J Stavric S Buchanan B 1991 Assessment of two commercial agglutination kits for detecting Escherichia coli heat-labile enterotoxin Can. J. Microbiol. 37 877 880 10.1139/m91-151 1777863

상세보기
28 Otawa K Lee SH Yamazoe A Onuki M Satoh H Mino T 2007 Abundance, diversity, dynamics of viruses on microorganisms in activated sludge processes Microb. Ecol. 53 143 152 10.1007/s00248-006-9150-9 17165120

상세보기
29 Wu Q Liu W 2009 Determination of virus abundance, diversity and distribution in a municipal wastewater treatment plant Water Res. 43 1101 1109 10.1016/j.watres.2008.11.039 19095276

상세보기
30 Rohwer F Thurber RV 2009 Viruses manipulate the marine environment Nature 459 207 212 10.1038/nature08060 19444207

상세보기
31 Kim EJ Chang HJ Kwak S Park JH 2016 Virulence factors and stability of coliphages specific to Escherichia coli O157:H7 and to various E. coli infection J. Microbiol. Biotechnol. 20 2060 2066 10.4014/jmb.1609.09039 27713209

원문보기 상세보기
32 Dumke R Schröter-Bobsin U Jacobs E Röske I 2006 Detection of phages carrying the Shiga toxin 1 and 2 genes in waste water and river water samples Lett. Appl. Microbiol. 42 48 53 10.1111/j.1472-765X.2005.01809.x 16411919

상세보기
33 Imamovic L Ballested E Jofre J Muniesa M 2010 Quantification of Shiga toxin-converting bacteriophages in wastewater and in fecal samples by real-time quantitative PCR Appl. Environ. Microbiol. 76 5693 5701 10.1128/AEM.00107-10 20622134

상세보기
34 Pushpinder KL Joyjit S Divya J 2018 Characterization of bacteriophages targeting non-O157 Shiga toxigenic Escherichia coli J. Food Prot. 81 785 794 10.4315/0362-028X.JFP-17-460 29624104

상세보기
35 Rode TM Axelsson A Granum PE Heir E Holck A L'Abée-Lund TM 2011 High stability of Stx2 phage in food and under foodprocessing conditions Appl. Environ. Microbiol. 77 5336 5341 10.1128/AEM.00180-11 21685156

상세보기
36 Muniesa M Lucena F Jofre J 1999 Comparative survival of free Shiga toxin 2-encoding phages and Escherichia coli strains outside the gut Appl. Environ. Microbiol. 65 5615 5618 10.1128/AEM.65.12.5615-5618.1999 10584029

상세보기
37 Allué-Guardia A Martínez-Castillo A Muniesa M 2014 Persistence of infectious Stx bacteriophages after disinfection treatments Appl. Environ. Microbiol. 80 2142 2149 10.1128/AEM.04006-13 24463973

상세보기
38 Schmidt H 2001 Shiga-toxin-converting bacteriophages Res. Microbiol. 152 687 695 10.1016/S0923-2508(01)01249-9 11686382

상세보기
39 Yoo BB Liu Y Juneja V Huang L Hwang CA 2017 Effect of environmental stresses on the survival and cytotoxicity of Shiga toxinproducing Escherichia coli Food Qual. Safety 1 139 146 10.1093/fqsafe/fyx010

상세보기
40 Olesen I Lene L 2010 Relative gene transcription and pathogenicity of enterohemorrhagic Escherichia coli after long-term adaptation to acid and salt stress Int. J. Food Microbiol. 141 248 253 10.1016/j.ijfoodmicro.2010.05.019 20603024

상세보기

LOADING...

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format

연합인증

Characteristics of Bacteriophage Isolates and Expression of Shiga Toxin Genes Transferred to Non Shiga Toxin-Producing E. coli by Transduction 원문보기

Abstract ▼ AI-Helper

주제어

참고문헌 (40)

이 논문을 인용한 문헌

연구과제 타임라인

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

연관된 기능

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트

연합인증

Characteristics of Bacteriophage Isolates and Expression of Shiga Toxin Genes Transferred to Non Shiga Toxin-Producing E. coli by Transduction 원문보기

Abstract ▼ AI-Helper

주제어

참고문헌 (40)

이 논문을 인용한 문헌

연구과제 타임라인

전체(0) 논문(0) 특허(0) 보고서(0)

전체(0) 논문(0) 특허(0) 보고서(0)

관련 콘텐츠

원문 보기

원문 URL 링크

오픈액세스(OA) 유형

연관된 기능

이 논문과 함께 이용한 콘텐츠

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

선택된 텍스트